大鼠两种脊髓半切模型制备方法的改良及比较

doi:10.3969/j.issn.1674-5817.2014.01.002

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (1): 6-11.DOI: 10.3969/j.issn.1674-5817.2014.01.002

大鼠两种脊髓半切模型制备方法的改良及比较

赵富生, 张晓莉, 武庚, 陈斌, 李月珍, 金秀东, 张际绯

牡丹江医学院, 牡丹江 157011

收稿日期:2013-07-16 出版日期:2014-02-25 发布日期:2014-02-25
作者简介:赵富生(1973-), 男, 讲师, 硕士, 研究方向: 干细胞于脊髓损伤基础与临床研究;E-mail: zhaofusheng910@163.com
基金资助:
国家自然科学基金项目(81171467)

Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats

ZHAO Fu-sheng, ZHANG Xiao-li, WU Geng, CHEN Bin, LI Yue-zhen, JIN Xiu-dong, ZHANG Ji-fei

Mudanjiang Medical College, Mudanjiang 157011, China

Received:2013-07-16 Online:2014-02-25 Published:2014-02-25

摘要/Abstract

摘要： 目的比较两种大鼠脊髓半切模型制作方法的各自特点及其应用,为临床脊髓损伤研究提供适宜的动物模型。方法 60只SD大鼠随机分为脊髓半横切组(A 组,n = 20)、脊髓楔型块状半切组(B 组,n = 20)和正常对照组(C组,n = 20)。术后采用BBB动功能评分、斜板实验、HE染色和MRI检测综合评价两种脊髓半切模型的稳定性。结果术后A组和B组大鼠伤侧后肢BBB运动功能评分及斜板维持最大角度值显著低于C组 (P<0.05),而这些指标在A组和B组之间没有明显差异; 术后28 d HE和MRI检测示A组和B组间脊髓损伤区组织结构具有显著差异。结论脊髓半横切与脊髓楔型半切法均可建立稳定、可靠的脊髓半切伤模型,而脊髓楔型半切模型更适于评价移植物治疗脊髓损伤的研究。

关键词: 脊髓半切, 动物模型, 大鼠

Abstract: Objective Comparing the characteristics and application of two methods for establishing the hemi-sected spinal cord injury model of rats and provide suitable spinal cord injury models for clinical research. Methods Sixty Sprague-Dawley rats were randomly divided into transverse-hemisected group (A group, n=20), wedge-shaped-block-hemisected group (B group, n=20) and normal control group (C group, n=20). After operation, the stability of the two hemi-sected models were comprehensive assessed by Basso-Beattie-Bresnahan (BBB) scores, inclined plane method, HE staining and MRI detection. Results After operation, the BBB scores and maximum angle of inclined Plane method of rats in group A and group B were significantly lower than group C (P<0.05), but these indexes had no significant difference between group A and group B; In addition, HE staining and MRI detection show a significant morphological change between group A and group B 28 days after operation. Conclusion Both methods of transverse-hemisected and wedge-shaped block-hemisected can establish a stable and reliable spinal cord injury model, and wedge-shaped-block-hemisected model is more suitable to assess graft treatment of spinal cord injury.

Key words: Hemi-sected spinal cord injury, Animal model, Rats

中图分类号:

Q95-33

赵富生, 张晓莉, 武庚, 陈斌, 李月珍, 金秀东, 张际绯. 大鼠两种脊髓半切模型制备方法的改良及比较[J]. 实验动物与比较医学, 2014, 34(1): 6-11.

ZHAO Fu-sheng, ZHANG Xiao-li, WU Geng, CHEN Bin, LI Yue-zhen, JIN Xiu-dong, ZHANG Ji-fei. Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats[J]. Laboratory Animal and Comparative Medicine, 2014, 34(1): 6-11.

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大鼠两种脊髓半切模型制备方法的改良及比较

Improvement and Comparison of Two Methods for Establishing Hemi-sected Spinal Cord Injury Model of Rats

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