痤疮动物模型建立及评价研究进展

doi:10.12300/j.issn.1674-5817.2023.021

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (4): 398-405.DOI: 10.12300/j.issn.1674-5817.2023.021

痤疮动物模型建立及评价研究进展

张睿¹()(), 吕美豫¹, 张建军¹()(), 刘金莲¹, 陈彦², 黄志强², 刘尧², 周澜华³()()

^1.北京中医药大学中医学院, 北京 102488
^2.北京中医药大学中药学院, 北京 102488
^3.北京京城皮肤医院皮肤科, 北京 100192

收稿日期:2023-02-17 修回日期:2023-04-10 出版日期:2023-08-25 发布日期:2023-09-14
通讯作者: 张建军（1965—），女，博士，教授，研究方向：中医优势病种防治及用药规律研究。E-mail：zhangjianjun@bucm.edu.cn。ORCID：0000-0003-3855-3359；
周澜华（1975—），女，硕士，副主任医师，研究方向：损容性皮肤病。E-mail：319950076@qq.com。ORCID：0000-0002-5660-8301
作者简介:张睿（1988—），男，博士研究生，研究方向：中医优势病种防治及用药规律研究。E-mail：zhangrui@bucm.edu.cn。ORCID：0000-0002-6500-6556
基金资助:
国家重点研发计划项目“基于辨证保健的中药复方保健产品评价技术体系研究及示范研发平台的建立”(2018YFC1706800)

Research Progress on Establishing and Evaluation of Acne Animal Models

Rui ZHANG¹()(), Meiyu LÜ¹, Jianjun ZHANG¹()(), Jinlian LIU¹, Yan CHEN², Zhiqiang HUANG², Yao LIU², Lanhua ZHOU³()()

^1.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
^2.School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
^3.Department of dermatology, Beijing Jingcheng Skin Hospital, Beijing 100192, China

Received:2023-02-17 Revised:2023-04-10 Published:2023-08-25 Online:2023-09-14
Contact: ZHANG Jianjun （ORCID：0000-0003-3855-3359）， E-mail：zhangjianjun@bucm.edu.cn；
ZHOU Lanhua （ORCID：0000-0002-5660-8301）， E-mail：319950076@qq.com；

摘要/Abstract

摘要：

近年来学者们根据对痤疮发生机制的认识建立了痤疮炎症动物模型、移植人皮痤疮动物模型和天然痤疮动物模型，其中炎症模型主要由细菌感染、化学药物涂抹和异物注射诱导，天然痤疮动物模型包括某些对激素敏感的动物和某些具有类似痤疮临床表现的动物。选择合适的模型动物和造模方法，对不同程度、不同机制的痤疮干预产品研发很有必要。目前我国的祛痤疮保健功能只有人体评价标准，无动物评价标准，一定程度上影响了痤疮发病机制的深入研究以及祛痤疮产品的研发进度。本文对痤疮产生的条件、人类皮肤特点、痤疮丙酸杆菌对人体皮肤的双向作用、痤疮动物模型、常用的观察与评价指标进行归纳总结，为痤疮发病机制研究、祛痤疮保健和治疗产品研发提供参考。

关键词: 痤疮, 动物模型, 研究进展

Abstract:

According to understanding of the pathogenesis of acne, scholars have established animal models of acne inflammation, animal models of grafting human skin acne, and natural acne animal models. The acne inflammation model is mainly induced by bacterial infection, chemical drug application, and foreign matter injection. Natural acne animal models include animals that some are sensitivity to hormones and some have clinical symptoms of acne. It is necessary to select appropriate model animals and replicate model methods for the development of acne intervention products with different degrees and mechanisms. At present, there are only human evaluation standards of acne health functions in China, but no animal evaluation standards, which has affected the in-depth study of the pathogenesis of acne as well as the research and development progress of acne products. This article summarizes the conditions for the occurrence of acne, the characteristics of human skin, the bidirectional effect of Cutibacterium acnes on human skin, acne animal models, and commonly used observation and evaluation indicators, providing the reference for studying the pathogenesis of acne, promoting acne treatment and health care, and developing treatment products.

Key words: Acne, Animal models, Research progress

中图分类号:

R-332

张睿, 吕美豫, 张建军, 刘金莲, 陈彦, 黄志强, 刘尧, 周澜华. 痤疮动物模型建立及评价研究进展[J]. 实验动物与比较医学, 2023, 43(4): 398-405.

Rui ZHANG, Meiyu LÜ, Jianjun ZHANG, Jinlian LIU, Yan CHEN, Zhiqiang HUANG, Yao LIU, Lanhua ZHOU. Research Progress on Establishing and Evaluation of Acne Animal Models[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 398-405.

图/表 2

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模型动物 Model animals	应用范围 Application range	优点 Advantages	缺点 Disadvantages
家兔 Rabbits	对粉刺、角质化异常的疗效评价；痤疮治疗、改善角质化的机制研究	研究最成熟的痤疮模型；兔的毛囊与人类相似；实验操作简单、易成模	兔耳皮肤的优势菌群与人类不同；无法做大量抗痤疮药物的筛选
大鼠 Rats	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	研究较成熟的痤疮模型；炎性反应明显	无法形成黑头粉刺
小鼠 Mice	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	体积小，成本低；炎性反应明显	耳廓注射操作不易；无法形成黑头粉刺；毛囊细胞类型与人类不同
豚鼠 Guinea pigs	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	致炎物质与人类角质细胞的微环境相同	致炎机制与人类痤疮炎性反应不同
裸鼠 Nude mice/rats	抗皮脂腺增生的疗效评价；皮脂代谢的机制研究	皮脂腺分布均匀	成本高；毛囊退化，缺少菌群生长环境；移植后死亡率高
金黄仓鼠 Golden hamsters	抗雄激素、抗皮脂腺增生的疗效评价；激素代谢和皮脂腺代谢的机制研究	可用于建立天然模型，对激素敏感	不易感染，无法做感染和炎性反应研究
犀牛小鼠 Rhino mice	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮	与人类角质不同，无法继发炎性反应；国内研究较少
墨西哥无毛犬 Mexican hairless dogs	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮；可以在身上不同部位筛选外用药	成本高；研究较少
小型猪 Miniature pigs	皮脂腺代谢的机制研究	可用于建立天然模型，与人类皮脂腺的氨基酮戊酸代谢一致	成本高；研究较少

模型动物 Model animals	应用范围 Application range	优点 Advantages	缺点 Disadvantages
家兔 Rabbits	对粉刺、角质化异常的疗效评价；痤疮治疗、改善角质化的机制研究	研究最成熟的痤疮模型；兔的毛囊与人类相似；实验操作简单、易成模	兔耳皮肤的优势菌群与人类不同；无法做大量抗痤疮药物的筛选
大鼠 Rats	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	研究较成熟的痤疮模型；炎性反应明显	无法形成黑头粉刺
小鼠 Mice	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	体积小，成本低；炎性反应明显	耳廓注射操作不易；无法形成黑头粉刺；毛囊细胞类型与人类不同
豚鼠 Guinea pigs	对炎症、角质化异常的疗效评价；抗炎、改善角质化的机制研究	致炎物质与人类角质细胞的微环境相同	致炎机制与人类痤疮炎性反应不同
裸鼠 Nude mice/rats	抗皮脂腺增生的疗效评价；皮脂代谢的机制研究	皮脂腺分布均匀	成本高；毛囊退化，缺少菌群生长环境；移植后死亡率高
金黄仓鼠 Golden hamsters	抗雄激素、抗皮脂腺增生的疗效评价；激素代谢和皮脂腺代谢的机制研究	可用于建立天然模型，对激素敏感	不易感染，无法做感染和炎性反应研究
犀牛小鼠 Rhino mice	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮	与人类角质不同，无法继发炎性反应；国内研究较少
墨西哥无毛犬 Mexican hairless dogs	对粉刺的疗效评价	可用于建立天然模型，能够自发产生类似人类粉刺的痤疮；可以在身上不同部位筛选外用药	成本高；研究较少
小型猪 Miniature pigs	皮脂腺代谢的机制研究	可用于建立天然模型，与人类皮脂腺的氨基酮戊酸代谢一致	成本高；研究较少

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痤疮动物模型建立及评价研究进展

Research Progress on Establishing and Evaluation of Acne Animal Models

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