一例Han-Wistar大鼠垂体细胞瘤的病理学分析

doi:10.12300/j.issn.1674-5817.2023.064

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (6): 654-658.DOI: 10.12300/j.issn.1674-5817.2023.064

一例Han-Wistar大鼠垂体细胞瘤的病理学分析

侯敏博, 崔甜甜, 苏娜瑛, 张苗苗, 焦永敏, 严建燕, 汪溪洁()(), 大平东子()()

上海益诺思生物技术股份有限公司, 上海 201203

收稿日期:2023-05-18 修回日期:2023-08-06 出版日期:2023-12-25 发布日期:2024-01-06
通讯作者: 汪溪洁（1979—），女，研究员，研究方向为药物非临床安全性评价。E-mail： xjwang@innostar.cn。ORCID： 0000-0001-7284-186X
作者简介:侯敏博（1987—），男，助理研究员，研究方向为药物非临床安全性评价。E-mail： mbhou@innostar.cn
大平东子（1960—），女，研究员，研究方向为药物非临床安全性评价。E-mail: tohira@innostar.cn。ORCID：0009-0004-3207-7886
基金资助:
上海市科委研发平台项目“新药安全评价专业技术服务平台”(21DZ2291000)

Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat

Minbo HOU, Tiantian CUI, Naying SU, Miaomiao ZHANG, Yongmin JIAO, Jianyan YAN, Xijie WANG()(), Ohira TOKO()()

Shanghai Innostar Bio-tech Co. , Ltd. , Shanghai 201203, China

Received:2023-05-18 Revised:2023-08-06 Published:2023-12-25 Online:2024-01-06
Contact: WANG Xijie (ORCID: 0000-0001-7284-186X), E-mail: xjwang@innostar.cn;

摘要/Abstract

摘要：

在为期两年的致癌试验中发现空白对照组Han-Wistar大鼠出现1例垂体细胞瘤。临床观察及大体剖检未见明显异常。HE染色结果显示，垂体神经部肿瘤组织呈结节状增生，边界清晰，压迫周围正常组织；肿瘤细胞与神经胶质细胞类似，细胞核呈圆形或卵圆形，细胞质丰富呈嗜酸性或空泡状；肿瘤细胞分化良好，细胞多形性不明显，核分裂象可见；部分肿瘤细胞呈假菊形团状排列。免疫组织化学染色结果显示，胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）及S-100蛋白呈阳性。结合HE染色及免疫组织化学染色结果诊断该肿瘤为自发性良性垂体细胞瘤。

关键词: 垂体细胞瘤, Han-Wistar大鼠, 病理学诊断

Abstract:

A case of pituicytoma was observed in a Han-Wistar rat from the control group of a 2-year carcinogenicity study. No obvious abnormality were found in clinical observation and necropsy. Hematoxylin and Eosin (HE) staining results showed that nodular hyperplasia in the pars nervosa of the pituitary, which was well demarcated and compressed the adjacent normal tissue. The tumor cells were similar to the glial cells with round or oval nuclei, cytoplasm rich in eosinophilic or vacuole. The tumor cells differentiated well, with no obvious cell pleomorphism and visible mitotic figures. Some tumor cells were arranged in a pseudorosette formation. Immunohistochemical staining (IHC) analysis confirmed positive expression of Glial fibrillary acidic protein (GFAP) and S-100 protein. The tumor was diagnosed as the spontaneous benign pituicytoma combining the HE and IHC staining results.

Key words: Pituicytoma, Han-Wistar rat, Pathological diagnosis

中图分类号:

R739.4

侯敏博, 崔甜甜, 苏娜瑛, 张苗苗, 焦永敏, 严建燕, 汪溪洁, 大平东子. 一例Han-Wistar大鼠垂体细胞瘤的病理学分析[J]. 实验动物与比较医学, 2023, 43(6): 654-658.

Minbo HOU, Tiantian CUI, Naying SU, Miaomiao ZHANG, Yongmin JIAO, Jianyan YAN, Xijie WANG, Ohira TOKO. Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat[J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 654-658.

图/表 2

图1 Han-Wistar大鼠自发肿瘤的组织病理学结果（HE染色）注：A，肿瘤位于垂体神经部，边界清晰（比例尺大小为500 μm）；B，肿瘤组织压迫周围神经部组织及垂体中间部（比例尺大小为200 μm）；C，肿瘤细胞与神经胶质细胞类似（比例尺大小为100 μm）；D，可见肿瘤细胞呈假菊形团状排列（黑色箭头）（比例尺大小为100 μm）；E，图D的局部放大（黑色箭头）（比例尺大小为50 μm）；F，肿瘤细胞细胞质丰富，呈嗜酸性或空泡状，可见小胶质细胞样肿瘤细胞（黑色箭头）及核分裂象（黄色箭头）（比例尺大小为50μm）。

Figure 1 Histopathological results of a the tumor from a Han-Wistar rat （HE staining）Note：A, The well demarcated mass in the pars nervosa of the pituitary (scale bar: 500 μm); B, The mass compressed the adjacent neurohypophysis and the pars intermedia (scale bar: 200 μm); C, The tumor cells were similar to the glial cells (scale bar: 100 μm); D, Some tumor cells were arranged in a pseudorosette formation (scale bar: 100 μm); E, The magnification of figure D (scale bar: 50 μm); F, The cytoplasm of tumor cells were rich in eosinophilic or vacuolate. Microglial cells-like tumor cells (black arrow) and mitotic figures (yellow arrow) were present (scale bar: 50 μm).

图2 Han-Wistar大鼠自发肿瘤的免疫组织化学染色结果（DAB染色）注：A和B，抗胶质纤维酸性蛋白（GFAP）免疫组织化学染色结果呈阳性（比例尺大小分别为200 μm和100 μm）；C和D，S-100免疫组织化学染色结果呈阳性（比例尺大小分别为100 μm和50 μm）。

Figure 2 Immunohistochemistry staining of tumor from a Han-Wistar rat （DAB staining）Note：A-B， Positive immunohistochemical reaction with glial fibrillary acidic protein （GFAP）（scale bar： 200 μm or 100 μm）； C， Positive immunohistochemical reaction with S-100 protein （scale bar： 100 μm or 50 μm）.

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一例Han-Wistar大鼠垂体细胞瘤的病理学分析

Pathologic Diagnosis of a Pituicytoma in a Han-Wistar Rat

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