关键词: 帕金森疾病, 树鼩, 1-甲基-4-苯基-1, 2, 3, 6-四氢吡啶(MPTP), 动物模型, 行为学, Catwalk步态分析系统, FPA分析系统

Abstract: Objective To establish the acute Parkinson’s disease(PD) model of tree shrew, the Catwalk and force plate actimeter (FPA) detection system was used as a method to evaluate PD behavior. Methods Totally 14 adult conventional grade tree shrew(Tupaia belangeri), weighing (147.6±17.07) g, were randomly divided into model group (n=10) and control group (n=4). The PD model of tree shrews was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) drugs, and control group was injected with equal volume of saline. Results The acute PD model of tree shrews was successfully prepared. Compared with control group, the model group spatial utilization index of tree shrews decreased and the bout of low mobility (BLM) index increased significantly (P all<0.001) after 1 day dosing, indicate that the spatial utilization ability of the model group was impaired and the activity degree was decreased; The maximum contact mean intensity of the right front paws(RF), right hind paws (RH), left front paws (LF). the mean intensity of RF, RH, LF and force average of limbs are decline (P all<0.05).That indicate the increased muscle stiffness of tree shrews, which can be used as a quantitative indicator of PD gait analysis; Tremor indices 1 (0~15 Hz) was significantly enhanced (P<0.01), which could better simulate the clinical manifestations of PD patients with rest tremor. Conclusion MPTP induced tree shrews acute PD model can better simulate the early PD clinical manifestations. Catwalk and FPA analysis systems provide systematic, Objective. and reliable PD behavioral data.

Key words: Parkinson's disease(PD), Tree shrew, 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP), Animal models, Behavior, Catwalk gait analysis system, Force plate actimeter (FPA)