实验用鱼的福利研究现状及思考

doi:10.12300/j.issn.1674-5817.2023.069

摘要/Abstract

摘要：

近年来，随着生命科学的高速发展，实验用鱼在毒理学、遗传学、发育生物学和医学等研究中的使用量大幅增加，逐渐成为重要的新型模式生物。与此同时，实验用鱼的福利问题也日益得到关注。尽管与陆生实验动物相比，实验用鱼福利的研究水平还处于相对早期阶段，但欧美等发达国家已经建立了相应的法律法规，以保障实验用鱼在研究中的福利。本文阐述了实验用鱼福利研究的发展现状，讨论需要重视和提高福利的原因，从饲养阶段和实验阶段两方面深入探究影响福利的因素，并探索提高福利的对策，旨在促进我国实验用鱼福利的不断提升。

关键词: 实验用鱼, 动物福利, 饲养条件, 3Rs原则

Abstract:

In recent years, with the rapid development of life sciences, the use of laboratory fish in toxicology, genetics, developmental biology and medicine has increased dramatically, and they have gradually become important new model organisms. At the same time, the welfare of laboratory fish has also received increasing attention. Although the research level of experimental fish welfare is still in a relatively early stage compared to terrestrial experimental animals, developed regions such as Europe and America have established corresponding legal frameworks to safeguard the welfare of laboratory fish in research. This article elucidates the current developmental status of laboratory fish welfare, discusses the rationale behind the imperative to prioritize and enhance their welfare, deeply investigates factors influencing their welfare from the feeding stage and experimental stage. Moreover, it explores strategies for augmenting welfare standards, with the overarching aim of propelling the continual improvement of laboratory fish welfare in our country.

Key words: Laboratory fish, Animal welfare, Husbandry conditions, 3Rs principles

中图分类号:

Q95-33

冯丽萍, 朱琦, 林金杏. 实验用鱼的福利研究现状及思考[J]. 实验动物与比较医学, 2023, 43(5): 524-530.

Liping FENG, Qi ZHU, Jinxing LIN. Current Status and Reflection on the Study of Welfare for Laboratory Fish[J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 524-530.

参考文献 44

1	TANG D D, GENG F, YU C X, et al. Recent application of zebrafish models in atherosclerosis research[J]. Front Cell Dev Biol, 2021, 9:643697. DOI: 10.3389/fcell.2021.643697 .
2	GOESSLING W, SADLER K C. Zebrafish: an important tool for liver disease research[J]. Gastroenterology, 2015, 149(6):1361-1377. DOI: 10.1053/j.gastro.2015.08.034 .
3	SHARCHIL C, VIJAY A, RAMACHANDRAN V, et al. Zebrafish: a model to study and understand the diabetic nephropathy and other microvascular complications of type 2 diabetes mellitus[J]. Vet Sci, 2022, 9(7):312. DOI: 10.3390/vetsci9070312 .
4	ZHAO Y B, ZHANG K, SIPS P, et al. Screening drugs for myocardial disease in vivo with zebrafish: an expert update[J]. Expert Opin Drug Discov, 2019, 14(4):343-353. DOI: 10.1080/17460441.2019.1577815 .
5	PADURARU E, IACOB D, RARINCA V, et al. Zebrafish as a potential model for neurodegenerative diseases: a focus on toxic metals implications[J]. Int J Mol Sci, 2023, 24(4):3428. DOI: 10.3390/ijms24043428 .
6	GRIFFIN A, HAMLING K R, HONG S, et al. Preclinical animal models for dravet syndrome: seizure phenotypes, comorbidities and drug screening[J]. Front Pharmacol, 2018, 9:573. DOI: 10.3389/fphar.2018.00573 .
7	NEUFFER S J, COOPER C D. Zebrafish syndromic albinism models as tools for understanding and treating pigment cell disease in humans[J]. Cancers, 2022, 14(7):1752. DOI: 10.3390/cancers14071752 .
8	LI S, YEO K S, LEVEE T M, et al. Zebrafish as a neuroblastoma model: progress made, promise for the future[J]. Cells, 2021, 10(3):580. DOI: 10.3390/cells10030580 .
9	SINGHAL S S, GARG R, MOHANTY A, et al. Recent advancement in breast cancer research: insights from model organisms-mouse models to zebrafish[J]. Cancers, 2023, 15(11):2961. DOI: 10.3390/cancers15112961 .
10	LEI P Y, ZHANG W X, MA J H, et al. Advances in the utilization of zebrafish for assessing and understanding the mechanisms of nano-/ microparticles toxicity in water[J]. Toxics, 2023, 11(4):380. DOI: 10.3390/toxics11040380 .
11	RISSONE A, BURGESS S M. Rare genetic blood disease modeling in zebrafish[J]. Front Genet, 2018, 9:348. DOI: 10.3389/fgene.2018.00348 .
12	SILIC M R, ZHANG G J. Bioelectricity in developmental patterning and size control: evidence and genetically encoded tools in the zebrafish model[J]. Cells, 2023, 12(8):1148. DOI: 10.3390/cells12081148 .
13	CASSAR S, ADATTO I, FREEMAN J L, et al. Use of zebrafish in drug discovery toxicology[J]. Chem Res Toxicol, 2020, 33(1):95-118. DOI: 10.1021/acs.chemrestox.9b00335 .
14	PATTON E E, ZON L I, LANGENAU D M. Zebrafish disease models in drug discovery: from preclinical modelling to clinical trials[J]. Nat Rev Drug Discov, 2021, 20(8):611-628. DOI: 10.1038/s41573-021-00210-8 .
15	Canadian Council on Animal Care. Guidelines on: the care and use of fish in research, teaching and testing [EB/OL]. (2005) [2023-09-07]. https://ccac.ca/Documents/Standards/Guidelines/Fish.pdf.
16	冯丽萍, 李焱冬, 林金杏, 等. 欧美实验动物福利立法浅析[J]. 中国比较医学杂志, 2013, 23(12): 52-55. DOI: 10.3969.j.issn.1671.7856.2013.012.013 .
	FENG L P, LI Y D, LIN J X, et al. Brief analysis of European and American laboratory animal welfare legislation[J]. Chin J Comp Med, 2013, 23(12): 52-55. DOI: 10.3969.j.issn.1671.7856.2013.012.013 .
17	于燕, 刘吉宏, 朱凤仙, 等. 浅谈中美研究机构实验动物饲养管理和使用计划的差异性[J]. 实验动物与比较医学, 2014, 34(4): 335-338. DOI: 10.3969/j.issn.1674-5817.2014.04.015 .
	YU Y, LIU J H, ZHU F X, et al. Differences on care and use of laboratory animals in research institutions between China and USA[J]. Lab Anim Comp Med, 2014, 34(4): 335-338. DOI: 10.3969/j.issn.1674-5817.2014.04.015 .
18	MASON T J, MATTHEWS M. Aquatic environment, housing, and management in the eighth edition of the Guide for the Care and Use of Laboratory Animals: additional considerations and recommendations[J]. J Am Assoc Lab Anim Sci, 2012, 51(3):329-332.
19	VARGA Z M, EKKER S C, LAWRENCE C. Workshop report: zebrafish and other fish models-description of extrinsic environmental factors for rigorous experiments and reproducible results[J]. Zebrafish, 2018, 15(6):533-535. DOI: 10.1089/zeb.2018.29006.zol .
20	ALESTRÖM P, D'ANGELO L, MIDTLYNG P J, et al. Zebrafish: housing and husbandry recommendations[J]. Lab Anim, 2020, 54(3):213-224. DOI: 10.1177/0023677219869037 .
21	国家市场监督管理总局, 中国国家标准化管理委员会. 实验动物实验鱼质量控制: GB/T 39649—2020[S]. 北京: 中国标准出版社, 2020.
	State Administration for Market Regulation, Standardization Administration of the People's Republic of China. Laboratory animal—Quality control of laboratory fish: GB/T 39649-2020 [S]. Beijing: Standards Press of China, 2020.
22	林金杏, 高诚, 胡建华. 实验用鱼类福利的发展现状[J]. 中国比较医学杂志, 2012, 22(10): 59-63. DOI: 10.3969/j.issn.1671-7856.2012.010.014 .
	LIN J X, GAO C, HU J H. Development status of laboratory fish welfare[J]. Chin J Comp Med, 2012, 22(10): 59-63. DOI: 10.3969/j.issn.1671-7856.2012.010.014 .
23	BROWN C. Fish intelligence, sentience and ethics[J]. Anim Cogn, 2015, 18(1):1-17. DOI: 10.1007/s10071-014-0761-0 .
24	BRAITHWAITE V A, EBBESSON L O E. Pain and stress responses in farmed fish[J]. Rev Sci Tech, 2014, 33(1):245-253. DOI: 10.20506/rst.33.1.2285 .
25	REILLY S C, QUINN J P, COSSINS A R, et al. Behavioural analysis of a nociceptive event in fish: comparisons between three species demonstrate specific responses[J]. Appl Anim Behav Sci, 2008, 114(1-2):248-259. DOI: 10.1016/j.applanim. 2008.01.016 .
26	BROOM D M. Cognitive ability and awareness in domestic animals and decisions about obligations to animals[J]. Appl Anim Behav Sci, 2010, 126(1-2):1-11. DOI: 10.1016/j.applanim.2010.05.001 .
27	AGRILLO C, PETRAZZINI M E M, DADDA M. Illusory patterns are fishy for fish, too[J]. Front Neural Circuits, 2013, 7:137. DOI: 10.3389/fncir.2013.00137 .
28	BIBOST A L, BROWN C. Laterality influences cognitive performance in rainbowfish Melanotaenia duboulayi [J]. Anim Cogn, 2014, 17(5):1045-1051. DOI: 10.1007/s10071-014-0734-3 .
29	RUCINQUE D S, SOUZA A P O, MOLENTO C F M. Perception of fish sentience, welfare and humane slaughter by highly educated citizens of bogotá, Colombia and Curitiba, Brazil[J]. PLoS One, 2017, 12(1): e0168197. DOI: 10.1371/journal.pone.0168197 .
30	金仕容, 华叶, 訾化星, 等. 一种显著提高实验用斑马鱼繁殖效率和使用寿命的优化养殖方案[J]. 实验动物与比较医学, 2023, 43(3):297-306. DOI: 10.12300/j.issn.1674-5817.2023.004 .
	JIN S R, HUA Y, ZI H X, et al. An optimized experimental zebrafish breeding scheme for significantly enhancing reproductive efficiency and service life[J]. Lab Anim Comp Med, 2023, 43(3):297-306. DOI: 10.12300/j.issn.1674-5817.2023.004 .
31	SANDERS E, FARMER S C. Aquatic models: water quality and stability and other environmental factors[J]. ILAR J, 2020, 60(2):141-149. DOI: 10.1093/ilar/ilaa008 .
32	ARECHAVALA-LOPEZ P, CABALLERO-FROILÁN J C, JIMÉNEZ-GARCÍA M, et al. Enriched environments enhance cognition, exploratory behaviour and brain physiological functions of Sparus aurata [J]. Sci Rep, 2020, 10(1):11252. DOI: 10.1038/s41598-020-68306-6 .
33	TSANG B, GERLAI R T. Common aquarium plants as an enrichment strategy in zebrafish facilities[J]. Zebrafish, 2022, 19(6):218-223. DOI: 10.1089/zeb.2022.0036 .
34	BARCELLOS H H A, KOAKOSKI G, CHAULET F, et al. The effects of auditory enrichment on zebrafish behavior and physiology[J]. PeerJ, 2018, 6: e5162. DOI: 10.7717/peerj.5162 .
35	CHATIGNY F. The controversy on fish pain: a veterinarian's perspective[J]. J Appl Anim Welf Sci, 2019, 22(4):400-410. DOI: 10.1080/10888705.2018.1530596 .
36	SNEDDON L U, ROQUES J A C. Pain recognition in fish[J]. Vet Clin North Am Exot Anim Pract, 2023, 26(1):1-10. DOI: 10.1016/j.cvex.2022.07.002 .
37	CHATIGNY F, CREIGHTON C M, STEVENS E D. Updated review of fish analgesia[J]. J Am Assoc Lab Anim Sci, 2018, 57(1):5-12.
38	ANDERSEN L, RØNNESETH A, POWELL M D, et al. Defining piscine endpoints: towards score sheets for assessment of clinical signs in fish research[J]. Lab Anim, 2023, 57(4):455-467. DOI: 10.1177/00236772231156031 .
39	MATTHEWS M, VARGA Z M. Anesthesia and euthanasia in zebrafish[J]. ILAR J, 2012, 53(2):192-204. DOI: 10.1093/ilar. 53.2.192 .
40	KÖHLER A, COLLYMORE C, FINGER-BAIER K, et al. Report of workshop on euthanasia for zebrafish-a matter of welfare and science[J]. Zebrafish, 2017, 14(6):547-551. DOI: 10.1089/zeb.2017.1508 .
41	DAVIS A K, GARNER J P, CHU D K, et al. Propofol immersion As a euthanasia method for adult zebrafish (Danio rerio)[J]. Comp Med, 2022, 72(3):204-209. DOI: 10.30802/AALAS-CM-22-000050 .
42	RAMP D, BEKOFF M. Compassion as a practical and evolved ethic for conservation[J]. BioScience, 2015, 65(3):323-327. DOI: 10.1093/biosci/biu223 .
43	AMIOT C E, BASTIAN B. Toward a psychology of human-animal relations[J]. Psychol Bull, 2015, 141(1):6-47. DOI: 10. 1037/a0038147 .
44	TARAZONA A M, CEBALLOS M C, BROOM D M. Human relationships with domestic and other animals: one health, one welfare, one biology[J]. Animals, 2019, 10(1):43. DOI: 10.3390/ani10010043 .

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