《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（四）

doi:10.12300/j.issn.1674-5817.2023.142

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (6): 659-668.DOI: 10.12300/j.issn.1674-5817.2023.142

所属专题：动物实验设计及统计学方法

• 比较医学研究与报告规范 • 上一篇下一篇

《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（四）

李夏莹¹^,², 田永路³, 刘晓宇⁴, 卢选成⁵, 陈国元⁶, 卢晓⁷, 白玉⁸, 高静⁹, 李垚¹⁰, 韦玉生¹^,²()(), 庞万勇¹²()(), 陶雨风¹¹()()

^1.北京大学实验动物中心, 北京 100871
^2.北京大学生命科学学院, 北京 100871
^3.北京大学心理与认知科学学院, 北京 100871
^4.中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050
^5.中国疾病预防控制中心实验动物中心, 北京 102206
^6.中国科学院分子细胞科学卓越创新中心动物实验技术平台, 上海 200031
^7.迪哲医药股份有限公司, 上海 201203
^8.北京诺和诺德医药科技有限公司, 北京 102206
^9.上海交通大学医学院临床研究中心, 上海 200025
^10.上海交通大学医学院实验动物科学部, 上海 200025
^11.中国合格评定国家认可中心, 北京 100062
^12.赛诺菲公司全球研发中心转化体内模型研究平台, 北京 100022

收稿日期:2023-10-13 修回日期:2023-12-05 出版日期:2023-12-25 发布日期:2024-01-08
通讯作者: 韦玉生（1975—），男，博士，高级工程师，研究方向：实验动物医学。E-mail： yusheng.wei@pku.edu.cn。ORCID： 0009-0007-0479-9488；
庞万勇（1974—），男，兽医学博士，研究方向：实验动物医学E-mail： pang1yong@outlook.com。ORCID： 0000-0002-0724-2016；
陶雨风（1970—），女，博士，高级工程师，研究方向：分子免疫学。E-mail： taoyf@cnas.org.cn。ORCID： 0000-0003-1925-1048；
作者简介:李夏莹（1986—），女，博士，高级工程师，研究方向：实验动物医学。E-mail： lixiaying@pku.edu.cn
基金资助:
国家重点研发计划“实验动物共性关键质量评价技术标准研究”(2022YFF0711000);中国科学技术期刊编辑学会2023年翰笔计划医学中青年编辑科研项目“动物实验医学研究及报告规范化的国际对标（指南汉化推广）”(HBJH-2023-A16)

Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)

Xiaying LI¹^,², Yonglu TIAN³, Xiaoyu LIU⁴, Xuancheng LU⁵, Guoyuan CHEN⁶, Xiao LU⁷, Yu BAI⁸, Jing GAO⁹, Yao LI¹⁰, Yusheng WEI¹^,²()(), Wanyong PANG¹²()(), Yufeng TAO¹¹()()

^1.Laboratory Animal Research Center, Peking University, Beijing 100871, China
^2.School of Life Science, Peking University, Beijing 100871, China
^3.School of Psychological and Cognitive Sciences, Peking University, Beijing 100871, China
^4.National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China
^5.Laboratory Animal Centre, Chinese Center for Disease Control and Prevention, Beijing 102206, China
^6.Animal Core Facility, Center for Excellence in Molecular Cell Science, Chinese Academy of Science, Shanghai 200031, China
^7.Dizal Pharmaceuticals Co. , Ltd. , Shanghai 201203, China
^8.Novo Nordisk Research Centre China, Beijing 102206, China
^9.Clinical Research Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
^10.Department of Laboratory Animal Science, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
^11.China National Accreditation Service for Conformity Assessment, Beijing 100062, China
^12.Translational in Vivo Model Research Platform, Sanofi G&D, Beijing 100022, China

Received:2023-10-13 Revised:2023-12-05 Published:2023-12-25 Online:2024-01-08
Contact: WEI Yusheng (ORCID: 0009-0007-0479-9488), E-mail: yusheng.wei@pku.edu.cn;
PANG Wanyong (ORCID: 0000-0002-0724-2016), E-mail: pang1yong@outlook.com;
TAO Yufeng (ORCID: 0000-0003-1925-1048), E-mail: taoyf@cnas.org.cn;

摘要/Abstract

摘要：

提高生物医学研究结果的可重复性是一项重大挑战，研究人员透明且准确地报告其研究过程有利于读者对该研究结果的可靠性进行评估，进而重复该实验或在该成果的基础上进一步探索。ARRIVE 2.0指南是英国国家3Rs中心（NC3Rs）于2019年组织发布的一份适用于任何与活体动物研究报告相关的指导性清单，用以提高动物体内实验设计、实验实施和实验报告的规范性，以及动物实验结果的可靠性、可重复性和临床转化率。ARRIVE 2.0指南的使用不仅可以丰富动物实验研究报告的细节，确保动物实验结果信息被充分评估和利用，还可以使读者准确且清晰地了解作者所表述的内容，促进基础研究评审过程的透明化和完整性。本文是在国际期刊遵循ARRIVE 2.0指南的最佳实践基础上，对2020 年发表于PLoS Biology期刊上的ARRIVE 2.0指南完整解读版（https：//arriveguidelines.org）第四部分包括“推荐11条”里的第1～5条：“摘要”、“研究背景”、“研究目标”、“伦理声明”和“饲养场所和饲养”等内容进行了编译、解释和阐述，以期促进国内研究人员充分理解并使用ARRIVE 2.0指南，提高实验动物研究及报告的规范性，助推我国实验动物科技与比较医学研究的高质量发展。

关键词: 动物实验, ARRIVE 2.0 指南, ARRIVE 推荐11条, 摘要, 研究背景, 研究目标, 伦理声明, 饲养场所和饲养

Abstract:

Improving the reproducibility of biomedical research results is a major challenge.Transparent and accurate reporting of the research process enables readers to evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at https://arriveguidelines.org). The fourth part of the article includes the items 1-5 of ARRIVE 2.0 Recommended 11 section, which covers "Abstract" "Background" "Objectives" "Ethical statement" and "Housing and husbandry". Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers, enhance the standardization of experimental animal research and reporting, and promote the high-quality development of experimental animal technology and comparative medicine research in China.

Key words: Animal experiment, ARRIVE 2.0 guidelines, ARRIVE recommended 11, Abstract, Background, Objectives, Ethical statement, Housing and husbandry

中图分类号:

R-332

李夏莹, 田永路, 刘晓宇, 卢选成, 陈国元, 卢晓, 白玉, 高静, 李垚, 韦玉生, 庞万勇, 陶雨风. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（四）[J]. 实验动物与比较医学, 2023, 43(6): 659-668.

Xiaying LI, Yonglu TIAN, Xiaoyu LIU, Xuancheng LU, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yusheng WEI, Wanyong PANG, Yufeng TAO. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)[J]. Laboratory Animal and Comparative Medicine, 2023, 43(6): 659-668.

图/表 1

参考文献 218

103	王剑, 卢今, 马政文, 等.«动物研究:体内实验报告»即 ARRIVE 2.0 指南的解释和阐述(一)[J]. 实验动物与比较医学, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
	WANG J, LU J, MA Z W, et al. Explanation and elaboration for the ARRIVE Guidelines 2.0—reporting animal research and in vivo experiments ( Ⅰ) [J]. Lab Anim Comp Med, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
104	陈国元, 卢晓, 白玉,等.«动物研究:体内实验报告»即 ARRIVE 2.0 指南的解释和阐述(二)[J]. 实验动物与比较医学, 2023, 43(3): 324-333. DOI: 10.12300/j.issn.1674-5817.2023.042 .
	CHEN G Y, LU X, BAI Y, et al. Explanation and elaboration of the ARRIVE guidelines 2.0—reporting animal research and in vivo experiments ( Ⅱ) [J]. Lab Anim Comp Med, 2023, 43(3): 323-331. DOI: 10.12300/j.issn.1674-5817.2023.042 .
155	刘晓宇, 卢选成, 师晓萌, 等.«动物研究:体内实验报告»即 ARRIVE 2.0指南的解释与阐述(三)[J]. 实验动物与比较医学, 2023, 43(4): 446-456. DOI: 10.12300/j.issn.1674-5817.2023.039 .
	LIU X Y, LU X C, SHI X M, et al. Explanation and elaboration for the ARRIVE guidelines 2.0 — reporting animal research and in vivo experiments ( Ⅲ) [J]. Lab Anim Comp Med 2023, 43(4): 446- 456. DOI: 10.12300/j.issn.1674-5817.2023.039 .
156	HAYNES R B, MULROW C D, HUTH E J, et al. More informative abstracts revisited[J]. Ann Intern Med, 1990, 113(1):69-76. DOI: 10.7326/0003-4819-113-1-69 .
157	HAIR K, MACLEOD M R, SENA E S, et al. A randomised controlled trial of an Intervention to Improve Compliance with the ARRIVE guidelines (IICARus)[J]. Res Integr Peer Rev, 2019, 4:12. DOI: 10.1186/s41073-019-0069-3 .
158	PITKIN R M, Branagan M A, Burmeister L F. Accuracy of data in abstracts of published research articles[J]. JAMA, 1999, 281(12):1110. DOI: 10.1001/jama.281.12.1110 .
159	BOUTRON I, ALTMAN D G, HOPEWELL S, et al. Impact of spin in the abstracts of articles reporting results of randomized controlled trials in the field of cancer: the SPIIN randomized controlled trial[J]. J Clin Oncol, 2014, 32(36):4120-4126. DOI: 10.1200/JCO.2014.56.7503 .
160	BANNACH-BROWN A, PRZYBYŁA P, THOMAS J, et al. Machine learning algorithms for systematic review: reducing workload in a preclinical review of animal studies and reducing human screening error[J]. Syst Rev, 2019, 8(1):23. DOI: 10.1186/s13643-019-0942-7 .
161	KASHANGURA R, SENA E S, YOUNG T, et al. Effects of MVA85A vaccine on tuberculosis challenge in animals: systematic review[J]. Int J Epidemiol, 2015, 44(6):1970-1981. DOI: 10.1093/ije/dyv142 .
162	TOLEDO A C, SAKODA C P, PERINI A, et al. Flavonone treatment reverses airway inflammation and remodelling in an asthma murine model[J]. Br J Pharmacol, 2013, 168(7):1736-1749. DOI: 10.1111/bph.12062 .
163	DEBOER S P, GARNER J P, MCCAIN R R, et al. An initial investigation into the effects of isolation and enrichment on the welfare of laboratory pigs housed in the PigTurn^®system, assessed using tear staining, behaviour, physiology and haematology[J]. Animal Welf, 2015, 24(1):15-27. DOI: 10.7120/ 09627286.24.1.015 .
164	AVEY M T, FENWICK N, GRIFFIN G. The use of systematic reviews and reporting guidelines to advance the implementation of the 3Rs[J]. J Am Assoc Lab Anim Sci, 2015, 54(2):153-162.
165	HOOIJMANS C R, RITSKES-HOITINGA M. Progress in using systematic reviews of animal studies to improve translational research[J]. PLoS Med, 2013, 10(7): e1001482. DOI: 10.1371/journal.pmed.1001482 .
166	SENA E S, CURRIE G L, MCCANN S K, et al. Systematic reviews and meta-analysis of preclinical studies: why perform them and how to appraise them critically[J]. J Cereb Blood Flow Metab, 2014, 34(5):737-742. DOI: 10.1038/jcbfm.2014.28 .
167	DONNER D G, ELLIOTT G E, BECK B R, et al. Impact of diet-induced obesity and testosterone deficiency on the cardiovascular system: a novel rodent model representative of males with testosterone-deficient metabolic syndrome (TDMetS)[J]. PLoS One, 2015, 10(9): e0138019. DOI: 10.1371/journal.pone.0138019 .
168	WILLNER P. Validation criteria for animal models of human mental disorders: learned helplessness as a paradigm case[J]. Prog Neuropsychopharmacol Biol Psychiatry, 1986, 10(6):677-690. DOI: 10.1016/0278-5846(86)90051-5 .
169	VAN DER WORP H B, HOWELLS D W, SENA E S, et al. Can animal models of disease reliably inform human studies?[J]. PLoS Med, 2010, 7(3): e1000245. DOI: 10.1371/journal.pmed.1000245 .
170	HUNT R F, GIRSKIS K M, RUBENSTEIN J L, et al. GABA progenitors grafted into the adult epileptic brain control seizures and abnormal behavior[J]. Nat Neurosci, 2013, 16(6):692-697. DOI: 10.1038/nn.3392 .
171	HAMILTON N, SABROE I, RENSHAW S A. A method for transplantation of human HSCs into zebrafish, to replace humanised murine transplantation models[J]. F1000Res, 2018, 7:594. DOI: 10.12688/f1000research.14507.2 .
172	FESTING M F W, ALTMAN D G. Guidelines for the design and statistical analysis of experiments using laboratory animals[J]. ILAR J, 2002, 43(4):244-258. DOI: 10.1093/ilar.43.4.244 .
173	KIMMELMAN J, MOGIL J S, DIRNAGL U. Distinguishing between exploratory and confirmatory preclinical research will improve translation[J]. PLoS Biol, 2014, 12(5): e1001863. DOI: 10.1371/journal.pbio.1001863 .
174	CAMP D M, LOEFFLER D A, FARRAH D M, et al. Cellular immune response to intrastriatally implanted allogeneic bone marrow stromal cells in a rat model of Parkinson's disease[J]. J Neuroinflammation, 2009, 6:17. DOI: 10.1186/1742-2094-6-17 .
175	FALK H, FORDE P F, BAY M L, et al. Calcium electroporation induces tumor eradication, long-lasting immunity and cytokine responses in the CT26 colon cancer mouse model[J]. Oncoimmunology, 2017, 6(5): e1301332. DOI: 10.1080/2162402X.2017.1301332 .
176	KIM J D, CHUN A Y, MANGAN R J, et al. A conserved retromer-independent function for RAB-6.2 in C. elegans epidermis integrity[J]. J Cell Sci, 2019, 132(5): jcs223586. DOI: 10.1242/jcs.223586 .
177	MCGRATH J C, LILLEY E. Implementing guidelines on reporting research using animals (ARRIVE etc.): new requirements for publication in BJP[J]. Br J Pharmacol, 2015, 172(13):3189-3193. DOI: 10.1111/bph.12955 .
178	BAYNE K, TURNER P V. Animal welfare standards and international collaborations[J]. ILAR J, 2019, 60(1):86-94. DOI: 10.1093/ilar/ily024 .
179	REDFERN W S, TSE K, GRANT C, et al. Automated recording of home cage activity and temperature of individual rats housed in social groups: the Rodent Big Brother Project[J]. PLoS One, 2017, 12(9): e0181068. DOI: 10.1371/journal.pone.0181068 .
180	WANG X C, XUE Q H, YAN F X, et al. Ulinastatin protects against acute kidney injury in infant piglets model undergoing surgery on hypothermic low-flow cardiopulmonary bypass[J]. PLoS One, 2015, 10(12): e0144516. DOI: 10.1371/journal.pone.0144516 .
181	BERGHOF T L, VAN DER KLEIN S A S, ARTS J J, et al. Genetic and non-genetic inheritance of natural antibodies binding keyhole limpet hemocyanin in a purebred layer chicken line[J]. PLoS One, 2015, 10(6): e0131088. DOI: 10.1371/journal.pone.0131088 .
182	BAILOO J D, MURPHY E, VARHOLICK J A, et al. Evaluation of the effects of space allowance on measures of animal welfare in laboratory mice[J]. Sci Rep, 2018, 8(1):713. DOI: 10.1038/s41598-017-18493-6 .
183	KALLNIK M, ELVERT R, EHRHARDT N, et al. Impact of IVC housing on emotionality and fear learning in male C3HeB/FeJ and C57BL/6J mice[J]. Mamm Genome, 2007, 18(3):173-186. DOI: 10.1007/s00335-007-9002-z .
184	HOLMES A M, EMMANS C J, JONES N, et al. Impact of tank background on the welfare of the African clawed frog, Xenopus laevis (Daudin)[J]. Appl Animal Behav Sci, 2016, 185:131-136. DOI: 10.1016/J.APPLANIM.2016.09.005 .
185	PAVLIDIS M, DIGKA N, THEODORIDI A, et al. Husbandry of zebrafish, Danio rerio, and the cortisol stress response[J]. Zebrafish, 2013, 10(4):524-531. DOI: 10.1089/zeb.2012.0819 .
186	HASEMAN J K, NEY E, NYSKA A, et al. Effect of diet and animal care/housing protocols on body weight, survival, tumor incidences, and nephropathy severity of F344 rats in chronic studies[J]. Toxicol Pathol, 2003, 31(6):674-681. DOI: 10.1080/01926230390241927 .
187	MORRIS J K, BOMHOFF G L, STANFORD J A, et al. Neurodegeneration in an animal model of Parkinson's disease is exacerbated by a high-fat diet[J]. Am J Physiol Regul Integr Comp Physiol, 2010, 299(4): R1082-R1090. DOI: 10.1152/ajpregu.00449.2010 .
188	BAYOL S A, FARRINGTON S J, STICKLAND N C. A maternal 'junk food' diet in pregnancy and lactation promotes an exacerbated taste for 'junk food' and a greater propensity for obesity in rat offspring[J]. Br J Nutr, 2007, 98(4):843-851. DOI: 10.1017/S0007114507812037 .
189	GASKILL B N, GARNER J P. Stressed out: providing laboratory animals with behavioral control to reduce the physiological effects of stress[J]. Lab Anim (NY), 2017, 46(4):142-145. DOI: 10.1038/laban.1218 .
190	ROBINSON I, DOWDALL T, MEERT T F. Development of neuropathic pain is affected by bedding texture in two models of peripheral nerve injury in rats[J]. Neurosci Lett, 2004, 368(1):107-111. DOI: 10.1016/j.neulet.2004.06.078 .
191	KOKOLUS K M, CAPITANO M L, LEE C T, et al. Baseline tumor growth and immune control in laboratory mice are significantly influenced by subthermoneutral housing temperature[J]. Proc Natl Acad Sci USA, 2013, 110(50):20176-20181. DOI: 10.1073/pnas.1304291110 .
192	LAWRENCE C. The husbandry of zebrafish (Danio rerio): a review[J]. Aquaculture, 2007, 269(1-4):1-20. DOI: 10.1016/j.aquaculture.2007.04.077 .
193	DUKE J L, ZAMMIT T G, LAWSON D M. The effects of routine cage-changing on cardiovascular and behavioral parameters in male Sprague-Dawley rats[J]. Contemp Top Lab Anim Sci, 2001, 40(1):17-20.
194	PRAGER E M, BERGSTROM H C, GRUNBERG N E, et al. The importance of reporting housing and husbandry in rat research[J]. Front Behav Neurosci, 2011, 5:38. DOI: 10.3389/fnbeh.2011.00038 .
195	ROSENBAUM M D, VANDEWOUDE S, JOHNSON T E. Effects of cage-change frequency and bedding volume on mice and their microenvironment[J]. J Am Assoc Lab Anim Sci, 2009, 48(6):763-773.
196	KAPPEL S, HAWKINS P, MENDL M T. To group or not to group? good practice for housing male laboratory mice[J]. Animals (Basel), 2017, 7(12):88. DOI: 10.3390/ani7120088 .
197	VAN LOO P L, MOL J A, KOOLHAAS J M, et al. Modulation of aggression in male mice: influence of group size and cage size[J]. Physiol Behav, 2001, 72(5):675-683. DOI: 10.1016/s0031-9384(01)00425-5 .
198	ADAMS C E, TURNBULL J F, BELL A, et al. Multiple determinants of welfare in farmed fish: stocking density, disturbance, and aggression in Atlantic salmon (Salmo salar)[J]. Can J Fish Aquat Sci, 2007, 64(2):336-344. DOI: 10.1139/f07-018 .
199	BLEICH A, HANSEN A K. Time to include the gut microbiota in the hygienic standardisation of laboratory rodents[J]. Comp Immunol Microbiol Infect Dis, 2012, 35(2):81-92. DOI: 10.1016/j.cimid.2011.12.006 .
200	DAUCHY R T, DUPEPE L M, OOMS T G, et al. Eliminating animal facility light-at-night contamination and its effect on circadian regulation of rodent physiology, tumor growth, and metabolism: a challenge in the relocation of a cancer research laboratory[J]. J Am Assoc Lab Anim Sci, 2011, 50(3):326-336.
201	CHAPILLON P, MANNECHÉ C, BELZUNG C, et al. Rearing environmental enrichment in two inbred strains of mice: 1. effects on emotional reactivity[J]. Behav Genet, 1999, 29(1):41-46. DOI: 10.1023/A: 1021437905913 .
202	HENDERSHOTT T R, CRONIN M E, LANGELLA S, et al. Effects of environmental enrichment on anxiety-like behavior, sociability, sensory gating, and spatial learning in male and female C57BL/6J mice[J]. Behav Brain Res, 2016, 314:215-225. DOI: 10.1016/j.bbr.2016.08.004 .
203	Garner J P. Stereotypies and other abnormal repetitive behaviors: potential impact on validity, reliability, and replicability of scientific outcomes[J]. ILAR J, 2005, 46(2):106-117.DOI:10.1093/ilar.46.2.106 .
204	GROSS A N M, ENGEL A K J, WÜRBEL H. Simply a nest? Effects of different enrichments on stereotypic and anxiety-related behaviour in mice[J]. Appl Animal Behav Sci, 2011, 134(3-4):239-245. DOI: 10.1016/j.applanim.2011.06.020 .
205	WÜRBEL H. Ideal homes? Housing effects on rodent brain and behaviour[J]. Trends Neurosci, 2001, 24(4):207-211. DOI: 10.1016/s0166-2236(00)01718-5 .
206	AUVERGNE R, LERÉ C, BAHH B EL, et al. Delayed kindling epileptogenesis and increased neurogenesis in adult rats housed in an enriched environment[J]. Brain Res, 2002, 954(2):277-285. DOI: 10.1016/s0006-8993(02)03355-3 .
207	SALVARREY-STRATI A, WATSON L, BLANCHET T, et al. The influence of enrichment devices on development of osteoarthritis in a surgically induced murine model[J]. ILAR J, 2008, 49(4):23-30. DOI: 10.1093/ilar.49.3.e23 .
208	HANNAN A J. Environmental enrichment and brain repair: harnessing the therapeutic effects of cognitive stimulation and physical activity to enhance experience-dependent plasticity[J]. Neuropathol Appl Neurobiol, 2014, 40(1):13-25. DOI: 10.1111/nan.12102 .
209	BRAITHWAITE V A, SALVANES A G V. Environmental variability in the early rearing environment generates behaviourally flexible cod: implications for rehabilitating wild populations[J]. Proc Biol Sci, 2005, 272(1568):1107-1113. DOI: 10.1098/rspb.2005.3062 .
210	SORGE R E, MARTIN L J, ISBESTER K A, et al. Olfactory exposure to males, including men, causes stress and related analgesia in rodents[J]. Nat Methods, 2014, 11(6):629-632. DOI: 10.1038/nmeth.2935 .
211	NEVALAINEN T. Animal husbandry and experimental design[J]. ILAR J, 2014, 55(3):392-398. DOI: 10.1093/ilar/ilu035 .
212	National Research Council (US) Institute for Laboratory Animal Research. Guidance for the description of animal research in scientific publications[J]. ILAR J, 2014, 55(3):536-540. DOI: 10.1093/ilar/ilu070 .
213	KOTLOSKI R J, SUTULA T P. Environmental enrichment: evidence for an unexpected therapeutic influence[J]. Exp Neurol, 2015, 264:121-126. DOI: 10.1016/j.expneurol.2014.11.012 .
214	JENSEN T L, KIERSGAARD M K, SØRENSEN D B, et al. Fasting of mice: a review[J]. Lab Anim, 2013, 47(4):225-240. DOI: 10.1177/0023677213501659 .
215	National Research Council. Guide for the Care and Use of Laboratory Animals[M]. 8th ed. Washington (DC): National Academies Press, 2011.
216	European Commission. Directive 2010/63/EU of the Euro-pean Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes[J/OL]. Official Journal of the European Union, 2010 [2020-06-16].https://eur-lex.europa.eu/legalcontent/EN/TXT/?uri=celex%3A32010L0063.
217	HEYKANTS M, MAHABIR E. Estrous cycle staging before mating led to increased efficiency in the production of pseudopregnant recipients without negatively affecting embryo transfer in mice[J]. Theriogenology, 2016, 85(5):813-821. DOI: 10.1016/j.theriogenology.2015.10.027 .
218	GALLASTEGUIA, CHEUNGJ, SOUTHARDT, et al. Volumetric and linear measurements of lung tumor burden from non-gated micro-CT imaging correlate with histological analysis in a genetically engineered mouse model of non-small cell lung cancer[J]. Lab Anim, 2018, 52(5):457-469. DOI: 10.1177/0023677218756457 .

编号No.	报告信息 Information to report	影响实验动物的示例 Examples of effects on laboratory animals
1	笼/箱/饲养系统（类型和尺寸）	影响行为^[182]和恐惧学习^[183]；水箱颜色影响水生物种的应激^[184-185]
2	食物和水（类型、成分、供应商和获取途径）	影响体重、肿瘤发展、肾病严重程度^[186]和出现帕金森病症状的阈值^[187]；母本的饮食会影响后代的体重^[188]
3	垫料和筑巢材料	影响对应激^[189]和疼痛^[190]的行为反应
4	温度和湿度	改变肿瘤发展进展^[191]；调节斑马鱼的性别分化^[192]
5	卫生（笼具更换/水箱换水频率、材料转移、水质）	影响血压、心率和行为^[193]；增加一个额外的变异源^[194-195]
6	社会环境（群体规模和组成/饲养密度）	损害动物福利^[196]；诱发攻击性行为^[197-198]和应激^[185]
7	生物安全（级别）	动物的微生物状态会导致系统性疾病参数的变化^[199]
8	照明（类型、时间表和强度）	改变免疫和应激反应^[200]
9	环境丰富	减少焦虑^[201-202]、应激^[201-202]和异常重复行为^[203-205]；降低癫痫^[206]和骨关节炎^[207]的易感性，并改变神经系统疾病的病理学^[208]；增加鱼类的觅食行为^[209]
10	实验动物的性别	影响生理应激和疼痛行为^[210]

编号No.	报告信息 Information to report	影响实验动物的示例 Examples of effects on laboratory animals
1	笼/箱/饲养系统（类型和尺寸）	影响行为^[182]和恐惧学习^[183]；水箱颜色影响水生物种的应激^[184-185]
2	食物和水（类型、成分、供应商和获取途径）	影响体重、肿瘤发展、肾病严重程度^[186]和出现帕金森病症状的阈值^[187]；母本的饮食会影响后代的体重^[188]
3	垫料和筑巢材料	影响对应激^[189]和疼痛^[190]的行为反应
4	温度和湿度	改变肿瘤发展进展^[191]；调节斑马鱼的性别分化^[192]
5	卫生（笼具更换/水箱换水频率、材料转移、水质）	影响血压、心率和行为^[193]；增加一个额外的变异源^[194-195]
6	社会环境（群体规模和组成/饲养密度）	损害动物福利^[196]；诱发攻击性行为^[197-198]和应激^[185]
7	生物安全（级别）	动物的微生物状态会导致系统性疾病参数的变化^[199]
8	照明（类型、时间表和强度）	改变免疫和应激反应^[200]
9	环境丰富	减少焦虑^[201-202]、应激^[201-202]和异常重复行为^[203-205]；降低癫痫^[206]和骨关节炎^[207]的易感性，并改变神经系统疾病的病理学^[208]；增加鱼类的觅食行为^[209]
10	实验动物的性别	影响生理应激和疼痛行为^[210]

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《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（四）

Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅳ)

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