关键词: 神经源性膀胱(NB), 脊髓损伤(SCI), 动物模型, SD大鼠, 膀胱内压

Abstract: Objective To establish the ideal animal model of neurogenic bladder (NB) after spinal cord injury (SCI) and evaluate and the state of the bladder. Methods Sixteen female SD rats were divided into control group and the experimental group (T9 spinal cord transection injury model), according to the record of the daily manual urination output to evaluate the NB recovery after SCI. After two weeks the simultaneous recording of the intravesical pressure were used to detect, compare and assess the state of bladders in two groups. Results The urine output of experimental rats at first day, 5^th day, 10^th day, 13^th day, 14^th day after operation were significantly higher than thoes of control group rats (P<0.05), and the urine output of experimental rats at 16^th day after operation was no statistically significant difference with control group (P>0.05). During a week postoperation, the daily manual urination output increased and reached maximum. One week later postoperation, the daily manual urination output was on the gradually decrease and stable for two weeks. The values of the parameters of the maximum bladder pressure, the basis bladder pressure, voiding threshold, bladder capacity, voiding efficiency in control group and experimental group respectively were 26.60±4.31 mmH₂O and 21.66±2.56 mmH₂O, 11.66±2.33 mmH₂O and 14.72±2.56 mmH₂O, 20.46±0.52 mmH₂O and 16.99±0.81 mmH₂O, 1.36±1.58 min and 2.02±0.36 min, 0.82±0.15 mL and 2.20±0.24 mL, 92.67%±1.97% and 25.33%±4.46%, the differences were statistically significant (P<0.05). Conclusion The NB rat model of T9 level spinal cord transection injury with a strong maneuverability and easy to quantify, repeatable. With the correct postoperative nursing care, there were fewer complications and low mortality. The recording of daily manual urination output and the intravesical pressure can be the good ways to detect the spinal cord recovery and evaluate the status of the bladder.

Key words: Neurogenic bladder (NB), Spinal cord injury (SCI), Animal models, Sprague-Dawley rats, Cystometric