关键词: Lewis肺癌, 模型, 病证结合

Abstract: Objective To establish a mice model of lung cancer with lung and kidney deficiency. Methods A total of 24 eight-week-old male C57BL/6 mice were randomly divided into normal group, Lewis lung cancer model group (model group)and Lewis lung cancer with lung and kidney deficiency group (combined group),with 8 mice in each group. Mice in normal group received no treatment, mice in model group were injected subcutaneously to the right axilla with Lewis lung cancer cells. The combined group were injected with Lewis lung cancer cells and hydrocortisione (25mg/kg, 1 times/day, × 14 days) and stimulated with cigarettes (2 times/days, × 14 days).The model of lung cancer with lung and kidney deficiency was determined by observing behaviors and physical signs of the mice, detecting the changes of feed consumption, body weight, survival time, tumor weight, spleen index, thymus index, lung index and histopathology. The changes of number of CD4⁺CD25⁺Foxp3⁺Treg cells and the expression of Foxp3 mRNA in spleen, thymus and tumor were detected by flow cytometer and real-time PCR. Results Compared with those in the normal group and model group, the mice in the combined group were noted with obvious signs of lung and kidney deficiency, such as activity less, sleepless, curly, hair loss, tiredness, obvious weight loss, rapid shallow breathing and urinating more than the amount of the performance, et al. Especially, the median survival time in combined group was significantly reduced 19 (13-33) d (P<0.01). The number of CD4⁺CD25⁺Foxp3⁺Treg cells in spleen and thymus was higher in combined group than in normal group and model group (P<0.01). The expression of Foxp3 mRNA in spleen and thymus was significantly up-regulated, compared with those in combined group than in normal group and model group (P<0.01). Conclusion The mice model of lung cancer with lung and kidney deficiency is established. It is provided an experimental tool for the study the mechanism Traditional Chinese Medicine treatment with lung cancer.

Key words: Lewis lung cancer, Model, Combining Diseases with syndromes