关键词: 血流阻断, 缺血再灌注, 动物模型

Abstract: Objective To establish the model of rat hepatic blood inflow occlusion without hemihepatic artery control. Methods Animal models were established by three surgical pathways. Pringle maneuver (PR), hemihepatic vascular occlusion (HH) and hepatic blood inflow occlusion without hemihepatic artery control (WH). The operation success rate, rat mortality , rat survival rate, liver pathological changes with 45 minutes hepatic ischemia followed by 24 hour reperfusion were observed. Results Thirty-three Wistar rats were operated and in each group 8 rats had been operated successfully. Operation success rate were respectively 88.9% (8/9) in group PR, 57.1% (8/14) in group HH, 80.8% (8/10) in group WH, the rat survival rate in group PR was only 37.5% (3/8) (hepatocyte spotty necrosis, ballon degeneration and acidophilic necrosis were observed), it was 100% (8/8) in group HH, and 87.5 % (7/8) in group WH. Mild liver pathological changes were obser ued in group HH and WH compared to group PR. Conclusion Hepatic blood inflow occlusion without hemihepatic artery control is a more simple and safe surgical pathway to establish rat hepatic bloodinflow occlusion model.

Key words: Vascular occlusion, Ischemia-reperfusion, Animal model