宫腔粘连大鼠模型研究进展

doi:10.12300/j.issn.1674-5817.2022.025

实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (6): 560-565.DOI: 10.12300/j.issn.1674-5817.2022.025

宫腔粘连大鼠模型研究进展

黄少凤¹(), 林忠²()(), 朱雪红², 刘姝岑¹, 宾力²

^1.广西中医药大学, 南宁 530200
^2.广西壮族自治区生殖医院, 南宁 530218

收稿日期:2022-03-04 修回日期:2022-10-21 出版日期:2022-12-25 发布日期:2023-01-04
通讯作者: 林忠（1968—），女，医学博士，主任医师，研究方向：妇科微创诊疗及生殖整复诊疗。E-mail： uters@126.com。ORCID： 0000-0002-4661-306X
作者简介:黄少凤（1994—），女，硕士研究生，主要研究领域为妇产科学（生殖医学）。E-mail： 1049376121@qq.com
基金资助:
广西壮族自治区卫生健康委员会自筹经费科研课题“自体月经血来源子宫内膜干细胞宫腔内移植治疗中重度宫腔粘连的研究”(Z20190031);广西医疗卫生适宜技术开发与推广应用项目“HA-Gel对中度IUA术后子宫内膜厚度、动脉血流、ER、PR、CD38、CD138表达的影响”(S2021033)

Research Progress in Rat Models of Intrauterine Adhesion

Shaofeng HUANG¹(), Zhong LIN²()(), Xuehong ZHU², Shuchen LIU¹, Li BIN²

^1.Guangxi University of Chinese Medicine, Nanning 530200, China
^2.The Reproductive Hospital of Guangxi Zhuang Autonomous Region, Nanning 530218, China

Received:2022-03-04 Revised:2022-10-21 Published:2022-12-25 Online:2023-01-04
Contact: LIN Zhong (ORCID: 0000-0002-4661-306X), E-mail: uters@126.com

摘要/Abstract

摘要：

完整的内膜及适当的内膜厚度是子宫生殖功能正常的必要条件。宫腔粘连（intrauterine adhesion，IUA）可致患者反复流产及不孕等，严重影响患者的正常生育功能。目前，对于IUA的治疗方式有手术消除、使用雌激素等，但存在治疗效率较低、易复发、妊娠率仍然较低等问题。IUA发生、发展的具体机制及子宫内膜受损后的修复机制尚不明晰，建立合适的动物模型有助于开展人类IUA各方面的研究。本文介绍了近些年IUA大鼠模型的造模方法，包括物理机械损伤法、化学法、生物损伤法、联合损伤法，然后从模型成模率、稳定性、IUA发病机制等方面阐述其适用情况，并探讨评价建模成功与否的观察指标，以期为从事相关领域研究的专业人员选择合适的造模方法提供有益的参考。总的来说，妊娠大鼠的反复内膜受损与感染联合模型或许能更贴合临床多次流产、刮宫术后合并感染引起的IUA。

关键词: 宫腔粘连, 动物模型, 大鼠

Abstract:

Intact endometrium and proper endometrial thickness are the necessary conditions for normal uterine reproductive function. Intrauterine adhesion (IUA) can cause recurrent abortion and infertility, which seriously affects the normal reproductive function of patients. At present, the treatment methods for IUA include surgery elimination and use of estrogen, but there are problems such as poor effectiveness, high rate of recurrence, and still with low pregnancy rate. The specific pathogenesis and developmental mechanism of IUA and the repair mechanism of damaged endometrium are still unclear. Establishing an appropriate animal model is conducive to the research of human IUA in all aspects. Here, we introduced the common rat models of IUA in recent years, including physical and mechanical damage method, chemical damage method, biological damage method and combined damage method, and described its application from the aspects of modeling rate, stability, and pathogenesis of IUA, and discussed the observation indexes to evaluate the success of modeling, in order to provide useful reference for selecting appropriate modeling methods. Generally, the combined model of repeated endometrial damage and infection of pregnant rats may be more suitable for the clinical IUA caused by multiple abortions and infections after curettage.

Key words: Intrauterine adhesion, Animal model, Rat

中图分类号:

Q95-33

黄少凤, 林忠, 朱雪红, 刘姝岑, 宾力. 宫腔粘连大鼠模型研究进展[J]. 实验动物与比较医学, 2022, 42(6): 560-565.

Shaofeng HUANG, Zhong LIN, Xuehong ZHU, Shuchen LIU, Li BIN. Research Progress in Rat Models of Intrauterine Adhesion[J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 560-565.

图/表 2

参考文献 31

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动物种类 Animal species	繁殖能力 Fertility	生殖周期 Reproductive cycle	价格 Price	遗传背景 Genetic background	操作难度 Operation difficulty	试验周期 Test cycle
小鼠 Mouse	生育力强，多产	妊娠期及经期短	低廉	来源明确，基因型稳定	易操作	短
大鼠 Rat	生育力强，多产	妊娠期及经期短	低廉	来源明确，基因型稳定	易操作	短
新西兰白兔 New Zealand white rabbit	生育力强，多产	妊娠期长短不定（32～36 d)	一般	来源明确，基因型稳定	易操作	短
比格犬 Beagle	季节性繁殖，每窝产仔数相对较少	妊娠期约55～65 d，经期约7～14 d	较高	有近亲繁殖因素存在，组织学背景资料不足	难操作	中等
非人灵长类 Non-human primate	每胎产仔数相对很少	妊娠期及经期与人类相近	昂贵	组织学背景资料不足	难操作	长

动物种类 Animal species	繁殖能力 Fertility	生殖周期 Reproductive cycle	价格 Price	遗传背景 Genetic background	操作难度 Operation difficulty	试验周期 Test cycle
小鼠 Mouse	生育力强，多产	妊娠期及经期短	低廉	来源明确，基因型稳定	易操作	短
大鼠 Rat	生育力强，多产	妊娠期及经期短	低廉	来源明确，基因型稳定	易操作	短
新西兰白兔 New Zealand white rabbit	生育力强，多产	妊娠期长短不定（32～36 d)	一般	来源明确，基因型稳定	易操作	短
比格犬 Beagle	季节性繁殖，每窝产仔数相对较少	妊娠期约55～65 d，经期约7～14 d	较高	有近亲繁殖因素存在，组织学背景资料不足	难操作	中等
非人灵长类 Non-human primate	每胎产仔数相对很少	妊娠期及经期与人类相近	昂贵	组织学背景资料不足	难操作	长

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宫腔粘连大鼠模型研究进展

Research Progress in Rat Models of Intrauterine Adhesion

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