关键词: 高原, 多脏器功能障碍综合征, 内毒素, 动物模型

Abstract: Objective To establish an simple and effective animal model of multiple organ dysfunction syndrome (MODS) induced by lipopolysaccharide (LPS) on highland scene in sheep. Methods Sheep was divided into the control, GroupⅠand Group. After anaesthetizing, 3 μg/kg and 6 μg/kg LPS were intravenously infused into GroupⅠand GroupⅡ sheep in 30 minutes and 60 minutes respectively. Sheep in the control group was treated by isotonic Na chloride in the same method. Then the indexes were determined in blood gas, the number of white blood cell (blood routine WBC) and blood biochemistry at the different time point. Results After intravenous injecting LPS, the death rate was 50% and 70% in GroupⅠand GroupⅡrespectively. The oxygenation index was evidently descendent (P<0.05 or P<0.01), creatine kinase (CK), creatine kinase isozyme (CK-MB), aspartic transaminase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL) and creatinine (CRE) were conspicuously ascendant (P<0.05 or P<0.01) at the different time point. But CK and CK-MB were obviously descendent (P<0.05 or P<0.01) from 48 h to 72 h. The number of white blood cell (WBC) was obviously decreased in 3 hours after intravenous injecting LPS, then it markedly increaed (P<0.05 or P<0.01). Conclusion The animal model of MODS was established by 3 μg/kg and 6 μg/kg of LPS successfully on highland scene.

Key words: Highland scene, Multiple organ dysfunction syndrome, Lipopolysaccharide, Animal model