Abstract: To establish the model of collagen-induced arthritis （CIA） and method of evaluation, the mice were injected subcutaneously with CFA （complete Freund’s adjuvant） and the change of secondary paw-swelling were observed and scored. The level of PGE was assayed by spectrophotometer； and the ³H-TdR incorporation was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin （IL）-1 and IL-2. Hepatic pathological changes was examined by Hematoxylin-eosin （HE） stain method. The results showed that the inflammation and paw swelling of treated mice increased significantly compared with those of control. Meanwhile, the ConA-induced splenocytes proliferation,PGE, IL-1 and IL-2 production were increased.The pathological examination showed that synovial cell increased ,pannus formed and inflammatory cells infiltrated into synovium. The results indicated that CIA mice model can be established by subcutaneous injection of CFA.