利用羟基磷灰石纳米管修复大鼠颅骨骨缺损实验观察

doi:10.3969/j.issn.1674-5817.2015.05.009

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (5): 390-393.DOI: 10.3969/j.issn.1674-5817.2015.05.009

利用羟基磷灰石纳米管修复大鼠颅骨骨缺损实验观察

朱彦丞, 郑欣, 陈一心, 王晓波, 邱旭升, 施鸿飞, 王骏飞, 桂雪洋

南京医科大学鼓楼临床医学院, 南京 210008

收稿日期:2015-05-05 出版日期:2015-10-25 发布日期:2015-10-25
作者简介:朱彦丞(1990-), 男, 骨外科学硕士, 研究方向: 骨创伤。E-mail: tonyzuyanjiu@163.com
基金资助:
国家自然科学基金项目(81401793); 江苏省社会发展基金项目(BE2011604); 江苏省六大人才高峰项目(2012-WS-092); 南京市卫生局项目(YKK13079、ZKX12016)

Experimental Observation on Repair of Calvarial Defects by Hydroxyapatite Nanotube

ZHU Yan-cheng, ZHENG Xin, CHEN Yi-xin, WANG Xiao-bo, QIU Xu-sheng, SHI Hong-fei, WANG Jun-fei, GUI Xue-yang

Department of Orthopedics, The Affiliated Drum Tower Hospital of Nanjing Medical University, Nanjing 210008, China

Received:2015-05-05 Online:2015-10-25 Published:2015-10-25

摘要/Abstract

摘要： 目的观察羟基磷灰石纳米管(HANT)对大鼠颅骨缺损修复成骨的作用。方法采用自主合成的HANT(专利号: Zl201010123277.5),选用12只3月龄SD大鼠,在其双侧颅骨顶部对称部位各建立直径为5 mm的缺损。采用自身对照的方法, 在大鼠左侧颅骨缺损内植入0.5 g HANT粉末为实验侧, 右侧为空白对照侧, 单纯制作颅骨缺损, 不植入任何材料。在术后的12周进行CT的影像学观察分析,术后12周处死大鼠,取出颅骨,行组织病理学分析。结果术后大鼠均未发生感染死亡; 术后各期植入区CT扫描实验侧颅骨缺损处均可见HANT粉末,密度较骨组织低; 病理学分析显示对照侧可见骨缺损处有纤维细胞填充,未见骨缺损愈合; 实验侧颅骨缺损内侧壁有少量软骨细胞生长,骨缺损处可见未降解HANT粉末,大量纤维细胞长入包裹材料。结论 HANT具有良好的生物相容性,但单纯应用HANT无法完全修复大鼠颅骨缺损,其成骨能力有待进一步实验改进及验证。

关键词: 羟基磷灰石纳米管(HANT), 大鼠, 颅骨缺损

Abstract: Objective To investigate the ostegenesis by hydroxyapatite nanotube(HANT) in a calvarial defect model. Methods Bilateral full-thickness defects (5 mm in diameter) of parietal bone were created in twelve three -month-old SD rats. The left defected side was filled with 0.5 g HANT (patent number: Zl201010123277.5) as experimental side and the right side was set as control. CT were taken at 12 weeks postoperation. The rats were euthanized at 12 weeks after surgery and the specimens were harvested for patholologic analysis. Results There was no infection or death on the surgical models. CT examination demonstrated that the residual HANT powder could be seen on the experimental side of the skull defect, but the density is lower than bone. Pathologic analysis showed that bone defect in the control side was filled with fiber cells. The powder partly degraded in the experimental side, few chondrocytes were found, and a large number of fiber cells enwrapped the powder. Conclusion The HANT has good biocompatibility, but singly implanting the nanotube can't completely repair the skull defect of rats. Therefore, its osteogenic ability need to be refined in further experiment.

Key words: Hydroxyapatite nanotube, Rats, Calvarial defect

中图分类号:

Q95-33

朱彦丞, 郑欣, 陈一心, 王晓波, 邱旭升, 施鸿飞, 王骏飞, 桂雪洋. 利用羟基磷灰石纳米管修复大鼠颅骨骨缺损实验观察[J]. 实验动物与比较医学, 2015, 35(5): 390-393.

ZHU Yan-cheng, ZHENG Xin, CHEN Yi-xin, WANG Xiao-bo, QIU Xu-sheng, SHI Hong-fei, WANG Jun-fei, GUI Xue-yang. Experimental Observation on Repair of Calvarial Defects by Hydroxyapatite Nanotube[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 390-393.

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利用羟基磷灰石纳米管修复大鼠颅骨骨缺损实验观察

Experimental Observation on Repair of Calvarial Defects by Hydroxyapatite Nanotube

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