缫丝水对老龄大鼠氧化应激水平的改善作用

doi:10.12300/j.issn.1674-5817.2022.027

摘要/Abstract

摘要：

目的探讨缫丝水对老龄大鼠氧化应激水平的改善作用。方法模拟传统缫丝水产生过程进行干蚕茧样品处理，并对缫丝水进行成分检测。采用16月龄老龄大鼠，根据血清丙二醛（malonaldehyde，MDA）含量将大鼠分层随机分为3个受试样品剂量组和1个阴性对照组，每组10只。剂量组分别给予500、250、125 mg/kg剂量的缫丝水，阴性对照组给予纯水，每天灌胃1次，连续灌胃30 d。实验结束后检测各组大鼠脂质氧化产物MDA、蛋白质氧化产物蛋白质羰基、抗氧化物质还原型谷胱甘肽（reduced glutathione，GSH）的含量和3种抗氧化酶即超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）的活性。结果缫丝水中粗蛋白含量为1 640 mg/100 g，游离氨基酸含量为18 mg/100 g，水解氨基酸含量为1 700 mg/100 g。丝氨酸（30.59%）、门冬氨酸（18.82%）、甘氨酸（10.00%）和苏氨酸（8.24%）是缫丝水中主要的水解氨基酸。与阴性对照组比较，缫丝水能降低老龄大鼠血清和组织中的MDA和蛋白质羰基含量（P＜0.05），提高血清和组织中的GSH含量（P＜0.05），提高组织中的SOD活性（P＜0.05），提高血清和组织中的CAT和GSH-Px活性（P＜0.05）。结论缫丝水具有改善老龄大鼠氧化应激的作用。

关键词: 缫丝水, 丝胶, 老龄大鼠, 氧化应激

Abstract:

Objective To investigate the capacity of silkworm cocoon water (SCW) to mitigate the level of oxidative stress in aged rats. Methods The samples were processed by simulating the traditional SCW production process, and the SCW components were examined. The 16-month-old rats were randomly divided into 3 experimental groups that were treated with different doses of SCW, and 1 negative control group according to serum malonaldehyde (MDA) content, with 10 rats in each group. Experimental groups were fed SCW at doses of 500, 250, 125 mg/kg body weight, respectively, and the negative control group was given pure water, once a day, for 30 days. At the end of the experiment, the contents of lipid oxidation products (MDA), protein oxidation products (carbonyl), antioxidant enzyme activities (SOD, CAT, GSH-Px), and antioxidant substances (GSH) were measured. Results The crude protein content of SCW was 1 640 mg/100 g, the free amino acid content was 18 mg/100 g, and the hydrolyzed amino acid content was 1 700 mg/100 g. Serine (30.59%), aspartate (18.82%), glycine (10.00%), and threonine (8.24%) were the main hydrolyzed amino acids in SCW. Compared with the negative control group, SCW reduced the contents of MDA and protein carbonyl in the serum and tissue (P < 0.05), but increased the contents of GSH in the serum and tissue, the activity of SOD in tissue, and the activity of CAT and GSH-Px in the serum and tissue of aged rats (P < 0.05). Conclusion SCW has the effect of improving oxidative stress in aged rats.

Key words: Silkworm cocoon water, Sericin, Aged rats, Oxidative stress

中图分类号:

R-332

覃辉艳, 陈华凤, 杨慧, 罗海兰, 傅伟忠, 李庆波, 张洁宏. 缫丝水对老龄大鼠氧化应激水平的改善作用[J]. 实验动物与比较医学, 2022, 42(5): 393-400.

Huiyan QIN, Huafeng CHEN, Hui YANG, Hailan LUO, Weizhong FU, Qingbo LI, Jiehong ZHANG. The Capacity of Silkworm Cocoon Water to Mitigate the Level of Oxidative Stress in Aged Rats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 393-400.

图/表 5

表1 缫丝水氨基酸成分

Table 1 Amino acid compositions of silkworm cocoon water

氨基酸种类 Amino acid varieties	百分含量 Contents /%
门冬氨酸 Asp	18.82
苏氨酸 Thr	8.24
丝氨酸Ser	30.59
谷氨酸Glu	6.47
脯氨酸Pro	0.82
甘氨酸 Gly	10.00
丙氨酸 Ala	4.18
缬氨酸Val	3.47
异亮氨酸 Ile	0.71
亮氨酸Leu	1.29
酪氨酸 Tyr	4.47
苯丙氨酸 Phe	0.41
赖氨酸 Lys	4.00
组氨酸 His	1.88
精氨酸 Arg	4.65
合计Total	100.00

表2 实验期间各组老龄大鼠的体质量

Table 2 Body weight of rats in each group during the experiment (xˉ±s)

剂量组 Dose group/ (mg·kg^-1)	动物数 Animal numbers	大鼠体质量 Body weight m/g									增质量 Weight gain m/g
剂量组 Dose group/ (mg·kg^-1)	动物数 Animal numbers	Day 1		Day 7		Day 14		Day 21		Day 30	增质量 Weight gain m/g
500	10	854.3±99.1	862.8±100.1		871.4±101.1		878.7±101.6		884.9±102.6		30.6±4.0
250	10	850.3±66.0	859.5±66.7		867.9±57.6		875.8±58.4		883.5±59.0		33.2±4.0
125	10	857.5±122.5	865.9±123.6		873.4±124.5		880.3±125.8		886.3±126.8		28.8±5.0
0	10	861.8±131.8	870.0±132.6		878.3±134.1		885.0±134.5		891.4±135.9		29.6±5.1

图1 缫丝水对老龄大鼠血清和脑、肝组织中MDA和蛋白质羰基含量的影响注：SCW为缫丝水。A为实验前血清丙二醛（MDA）含量；B为实验终血清MDA含量；C为实验终脑组织MDA含量；D为实验终肝组织MDA含量；E为实验终血清蛋白质羰基含量；F为实验终脑组织蛋白质羰基含量；G为实验终肝组织蛋白质羰基含量。每组10只大鼠，数据表示为平均值±标准差。与阴性对照组比较，*P＜0.05。

Figure 1 Effects of silkworm cocoon water on MDA levels and carbonyl contents in the serum， brain and liver of ratsNote：SCW, silkworm cocoon water. A, serum malonaldehyde (MDA) levels before the experiment; B, serum MDA levels at the end of the experiment; C, brain tissue MDA levels at the end of the experiment; D, hepatic tissue MDA levels at the end of the experiment; E, serum carbonyl contents at the end of the experiment; F, brain tissue carbonyl contents at the end of the experiment; G, hepatic tissue carbonyl contents at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

图2 缫丝水对老龄大鼠血清和脑、肝组织中GSH含量和GSH-Px活性的影响注：SCW为缫丝水。A为实验终血清还原型谷胱甘肽（GSH）含量；B为实验终脑组织GSH含量；C为实验终肝组织GSH含量；D为实验终血清谷胱甘肽过氧化物酶（GSH-Px）活性；E为实验终脑组织GSH-Px活性；F为实验终肝组织GSH-Px活性。每组10只大鼠，数据表示为平均值±标准差。与阴性对照组比较，*P＜0.05。

Figure 2 Effects of silkworm cocoon water on GSH levels and GSH-Px activity in the serum, brain, and liver of ratsNote：SCW, silkworm cocoon water. A, serum reduced glutathione （GSH） levels at the end of the experiment; B, brain tissue GSH levels at the end of the experiment; C, hepatic tissue GSH levels at the end of the experiment; D, serum glutathione peroxidase （GSH-Px） activity at the end of the experiment; E, brain tissue GSH-Px activity at the end of the experiment; F, hepatic tissue GSH-Px activity at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

图3 缫丝水对老龄大鼠血清及肝、脑组织SOD和CAT活性的影响注：SCW为缫丝水。A为实验终血清超氧化物歧化酶（SOD）活性；B为实验终脑组织SOD活性；C为实验终肝组织SOD活性；D为实验终血清过氧化物酶（CAT）活性；E为实验终脑组织CAT活性；F为实验终肝组织CAT活性。每组10只大鼠，数据表示为平均值±标准差。与阴性对照组比较，*P＜0.05。

Figure 3 Effects of silkworm cocoon water on SOD activity and CAT activity in the serum, brain, and liver of ratsNote：SCW, silkworm cocoon water. A, serum superoxide dismutase (SOD) activity at the end of the experiment; B, brain tissue SOD activity at the end of the experiment; C, hepatic tissue SOD activity at the end of the experiment; D, serum catalase (CAT) activity at the end of the experiment; E, brain tissue CAT activity at the end of the experiment; F, hepatic tissue CAT activity at the end of the experiment. Data are expressed as mean ± SD with 10 rats per group. *P<0.05, compared with the negative control group.

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