疏肝解郁方对便秘型肠易激综合征大鼠模型的疗效及脑-肠轴功能的影响

doi:10.12300/j.issn.1674-5817.2022.135

实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (6): 551-559.DOI: 10.12300/j.issn.1674-5817.2022.135

疏肝解郁方对便秘型肠易激综合征大鼠模型的疗效及脑-肠轴功能的影响

吴斌¹()(), 王旭², 付东旭³, 朱宇均⁴, 黄金路⁵, 朱顺星²()()

^1.南通市海门区人民医院康复医学科, 海门 226100
^2.南通大学实验动物中心, 南通 226001
^3.南通大学神经再生重点实验室, 南通 226001
^4.南通市海门区人民医院消化内科, 海门 226100
^5.上海交通大学医学院附属第六人民医院药剂科, 上海 200233

收稿日期:2022-08-29 修回日期:2022-12-09 出版日期:2022-12-25 发布日期:2023-01-04
通讯作者: 朱顺星（1968—），男，博士，副教授，研究方向：人类疾病动物模型。E-mail： zsx@ntu.edu.cn。ORCID：0000-0001-8011-4099
作者简介:吴斌（1976—），男，硕士，主治医师，研究方向：脾胃病、脑病的中西医结合药物及康复治疗。E-mail： wubinhm@126.com。ORCID： 0000-0001-7072-9495
基金资助:
2019年度南通市市级科技计划（指导性）项目“徐景藩经验方加减治疗便秘型肠易激综合征临床观察及对细胞因子的影响”(MSZ19081)

Effect of the Traditional Chinese Medicine Shuganjieyu Formula on Constipation Type Irritable Bowel Syndrome and Brain-gut Axis in Rats

Bin WU¹()(), Xu WANG², Dongxu FU³, Yujun ZHU⁴, Jinlu HUANG⁵, Shunxing ZHU²()()

^1.Department of Rehabilitation Medicine, Nantong Haimen People's Hospital, Haimen 226100, China
^2.Laboratory Animal Center, Nantong University, Nantong 226001, China
^3.Key Laboratory of Neural Regeneration, Nantong University, Nantong 226001, China
^4.Department of Gastroenterology, Nantong Haimen People's Hospital, Haimen 226100, China
^5.Department of Pharmacy, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China

Received:2022-08-29 Revised:2022-12-09 Published:2022-12-25 Online:2023-01-04
Contact: ZHU Shunxing (ORCID: 0000-0001-8011-4099), E-mail: zsx@ntu.edu.cn

摘要/Abstract

摘要：

目的观察疏肝解郁方对便秘型肠易激综合征（constipation irritable bowel syndrome，IBS-C）大鼠模型的干预效果及对5-羟色胺（5-hydroxytryptamine，5-HT）、促肾上腺皮质激素释放激素（corticotropin releasing hormone，CRH）和促肾上腺皮质激素（adrenocorticotropic hormone，ACTH）的影响，探讨其调控脑-肠轴功能的作用机制。方法将40只SD大鼠随机分为正常对照组、模型组及中药疏肝解郁方低、高剂量组，每组10只。除正常对照组外，其余各组采用冰水灌胃法制备IBS-C大鼠模型，待模型成功后低、高剂量中药组分别灌胃给予5 g/kg、20 g/kg的疏肝解郁方干预2周。观察各组大鼠体质量、粪便粒数及粪便含水量的变化情况，共28 d；末次给药后，取下腔静脉血收集血清，3%戊巴比妥钠溶液麻醉后截取结肠，光学显微镜下观察结肠组织形态学改变及肥大细胞（mast cells，MC）计数；免疫组织化学法检测各组大鼠结肠组织中5-HT阳性表达量；ELISA法检测各组大鼠血清中5-HT、CRH和ACTH的含量。结果与正常对照组相比，IBS-C模型组大鼠体质量增加减缓，粪便粒数减少，粪便含水量降低（P<0.05），结肠组织形态学未见有异常病理改变，结肠组织中MC细胞数量和5-HT阳性表达量显著增加（P<0.05），血清中5-HT的含量显著增加，而ACTH的含量显著降低（P<0.05）。与模型组相比，中药各剂量组大鼠体质量有所增加、粪便粒数及粪便含水量趋于正常，但差异不具有统计学意义，中药各剂量组大鼠结肠组织结构正常，MC细胞数量和5-HT阳性表达量均显著降低（P<0.05），血清中5-HT含量显著降低，而ACTH含量显著增加（P<0.05）。中药各剂量组间量效关系不具有统计学意义。结论疏肝解郁方能有效改善IBS-C模型大鼠的脑-肠轴功能紊乱，对IBS-C有良好的治疗作用。

关键词: 便秘型肠易激综合征, 疏肝解郁方, 脑-肠轴功能, 大鼠

Abstract:

Objective To observe the intervention effect of Shuganjieyu Formula on the rat model of constipation irritable bowel syndrome (IBS-C) and its effects on 5- hydroxytryptamine (5-HT), corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH), and to explore their mechanism of regulating brain-gut axis function. Methods Totally fourty rats were randomly divided into four groups with 10 rats in each group, including normal control group, model group, low (5 mg/kg) and high (20 mg/kg) doses of Shuganjieyu Formula groups. Except for the normal control group, the other three groups of rats were gavaged with ice water for preparing the IBS-C model. Subsequently, the low and high doses groups of rats were treated with Shuganjieyu Formula for 2 weeks via oral gavage. The body weight of rats, total particle number and water content of fecal in the four groups were observed for 28 days. At last, serum of inferior vena cava and colon were collected from rats after anesthesia of 3% pentobarbital sodium. The morphological changes of colon tissue and the count of mast cells (MC) were observed with light microscope. Immunohistochemistry was used to detect the positive expression of 5-HT in the colon of rats in each group. The serum levels of 5-HT, CRH and ACTH were detected by ELISA. Results Compared with the normal control group, the body weight of rats in IBS-C model group increased slowly, the number of fecal grains and the fecal water content decreased (P<0.05); the number of MC cells, the positive expression of 5-HT in colon tissue increased significantly in IBS-C group (P<0.05); the content of 5-HT in serum increased significantly, while the content of ACTH decreased significantly in IBS-C group (P<0.05). Colonic morphology showed no abnormal pathological changes in IBS-C model group. Compared with the model group, the body weight, fecal granule number and fecal water content of rats in each dose group of traditional Chinese medicine increased, but the differences were not statistically significant. The colon tissue structure of rats in each dose group of traditional Chinese medicine was normal. Compared with the model group, the number of MC cells and the positive expression of 5-HT in each dose group of traditional Chinese medicine decreased significantly (P<0.05), the content of 5-HT in serum decreased significantly, while the content of ACTH increased significantly (P<0.05). The dose-effect relationship between the low and high doses groups of Shuganjieyu Formula was not statistically significant. Conclusion The traditional Chinese medicine Shuganjieyu Formula can effectively improve the dysfunction of brain-gut axis in IBS-C model rats, and has a good therapeutic effect on IBS-C.

Key words: Constipation irritable bowel syndrome, Shuganjieyu Formula, Brain-gut axis function, Rats

中图分类号:

R-332

吴斌, 王旭, 付东旭, 朱宇均, 黄金路, 朱顺星. 疏肝解郁方对便秘型肠易激综合征大鼠模型的疗效及脑-肠轴功能的影响[J]. 实验动物与比较医学, 2022, 42(6): 551-559.

Bin WU, Xu WANG, Dongxu FU, Yujun ZHU, Jinlu HUANG, Shunxing ZHU. Effect of the Traditional Chinese Medicine Shuganjieyu Formula on Constipation Type Irritable Bowel Syndrome and Brain-gut Axis in Rats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 551-559.

图/表 4

图1 各组大鼠体质量、粪便粒数、粪便含水量变化趋势注：NC，正常对照组；Model，模型对照组；Low-dose，疏肝解郁方药物低剂量（5 g/kg）组；High-dose，疏肝解郁方药物高剂量（20 g/kg）组。A为各组大鼠体质量变化情况，与模型组相比，*P<0.05；各组样本数均为10。B为各组大鼠每日粪便粒数变化趋势；C为各组大鼠每日粪便含水量变化趋势；图B、C中，与正常对照组相比，*P<0.05；各组样本数均为10。

Figure 1 Change trend of body mass, fecal particles and fecal water content of rats in each groupNote： NC, normal control group; Model, model control group; Low-dose, Shuganjieyu Formula low-dose group (5 g/kg); High-dose, Shuganjieyu Formula high-dose group (20 g/kg). A: Changes in body mass of rats with time, compared to the model group, *P<0.05, n=10; B and C: Tendency of changes in daily feces and their water content in all groups of rats, compared to the normal control group, *P<0.05, n=10.

图2 大鼠结肠组织病理学变化（甲苯胺蓝改良法染色，×200）及肥大细胞计数注：左为结肠组织的甲苯胺蓝改良染色图（各小图的比例尺大小一致，均为50 μm），A为正常对照组（NC），B为模型组（Model），C为疏肝解郁方药物低剂量（5 g/kg）组（Low-dose），D为疏肝解郁方药物高剂量（20 g/kg）组（High-dose）；E为肥大细胞的阳性细胞计数统计图，与模型组相比，**P<0.01，***P<0.001；各组样本数均为10。

Figure 2 Histopathological changes (toloniumchloride，×200) and the number of mast cells in colon of ratsNote：Left, the results of modified toloniumchloride staining of colon tissue (the scale of each small figure was all 50 μm). A: Normal control group; B: Model control group; C and D: Low- (5 g/kg) and high- (20 g/kg) dose of Shuganjieyu Formula groups. E: The statistical data of mass cells positive cells, compared to the model group, **P<0.01，***P<0.001, n=10。

图3 大鼠结肠组织5-HT免疫组织化学染色（DAB染色，×200）注：左为结肠组织免疫组织化学染色图（DAB，各小图的比例尺大小一致，均为50 μm），A为正常对照组（NC），B为模型组（Model），C为疏肝解郁方药物低剂量（5 g/kg）组（Low-dose），D为疏肝解郁方药物高剂量（20 g/kg）组（High-dose）；E为5-羟色胺（5-HT）阳性表达面积半定量统计图，与模型组相比，**P<0.01；各组样本数均为10。

Figure 3 5-HT immunohistochemical staining of colon tissue of rats （DAB，×200 ）Note：On the left is the colon tissue immunohistochemical staining (DAB, all of which have the same scale size, 50 μm). A is the normal control group (NC), B is the model control group (Model), C is the low-dose (5 g/kg) group of Shuganjieyu Formula (low-dose), D is the high-dose (20 g/kg) group of Shuganjieyu Formula (high-dose). E: The semi-quantitative statistical graph of 5-hydroxytryptamine (5-HT) positive expression area, compared with the model group, **P<0.01, n=10。

图4 大鼠血清中5-HT、CRH、ACTH含量比较（ELISA法）注： ACTH，促肾上腺皮质激素；CRH，促肾上腺皮质激素释放激素；5-HT，5-羟色胺。NC，正常对照组；Model，模型对照组；Low-dose，疏肝解郁方药物低剂量（5 g/kg）组；High-dose，疏肝解郁方药物高剂量（20 g/kg）组。与模型组相比，ns表示P>0.05，*P<0.05，**P<0.01，***P<0.001；各组样本数均为10。

Figure 4 Comparison of 5-HT, CRH and ACTH contents in serum of rats in the four groups (ELISA)Note: ACTH, adrenocorticotropic hormone; CRH, corticotropin releasing hormone; 5-HT, 5-hydroxytryptamine. NC, Normal control group; Model, model control group; Low-dose, Shuganjieyu Formula low-dose group (5 g/kg); High-dose, Shuganjieyu Formula high-dose group (20 g/kg). Compared to the model group，ns indicates P>0.05，*P<0.05，**P<0.01，***P<0.001; n=10.

参考文献 25

1	刘建湘, 刘新光. 肠易激综合征的诊断与治疗[J]. 中国实用内科杂志, 2008, 28(7):524-526. DOI:10.3969/j.issn.1005-2194.2008.07.006 .
	LIU J X, LIU X G. Diagnosis and treatment of irritable bowel syndrome[J]. Chin J Pract Intern Med, 2008, 28(7):524-526. DOI:10.3969/j.issn.1005-2194.2008.07.006 .
2	MERTZ H R. Irritable bowel syndrome[J]. N Engl J Med, 2003, 349(22):2136-2146. DOI:10.1056/nejmra035579 .
3	中华医学会消化病学分会胃肠功能性疾病协作组, 中华医学会消化病学分会胃肠动力学组. 中国肠易激综合征专家共识意见(2015年, 上海)[J]. 中华消化杂志, 2016, 36(5):299-312. DOI:10.3760/cma.j.issn.0254-1432.2016.05.005 .
	Gastrointestinal Functional Diseases Collaborative Group of Digestive Branch of Chinese Medical Association, Gastro-intestinal Dynamics Group of Digestive Branch of Chinese Medical Association. Expert consensus on irritable bowel syndrome in China (Shanghai, 2015) [J]. Chin J Dig, 2016(5):299-312. DOI: 10.3760/cma. j.issn. 0254-1432.2016.05.005 .
4	宋怡然, 梁笑楠, 李忱阳, 等. «2020年中国肠易激综合征专家共识意见»解读[J]. 临床荟萃, 2021, 36(7): 628-631.
	SONG Y R, LIANG X N, LI C Y, et al. Interpretation on Chinese expert consensus of irritable bowel syndrome in 2020[J]. Clin Focus, 2021, 36(7): 628-631.
5	LEE C, DOO E, CHOI J M, et al. The increased level of depression and anxiety in irritable bowel syndrome patients compared with healthy controls: systematic review and Meta-analysis[J]. J Neurogastroenterol Motil, 2017, 23(3):349-362. DOI:10.5056/jnm16220 .
6	ZHANG Q E, WANG F, QIN G, et al. Depressive symptoms in patients with irritable bowel syndrome: a meta-analysis of comparative studies[J]. Int J Biol Sci, 2018, 14(11):1504-1512. DOI:10.7150/ijbs.25001 .
7	ZAMANI M, ALIZADEH-TABARI S, ZAMANI V. Systematic review with meta-analysis: the prevalence of anxiety and depression in patients with irritable bowel syndrome[J]. Aliment Pharmacol Ther, 2019, 50(2):132-143. DOI:10.1111/apt.15325 .
8	HU Z C, LI M X, YAO L, et al. The level and prevalence of depression and anxiety among patients with different subtypes of irritable bowel syndrome: a network meta-analysis[J]. BMC Gastroenterol, 2021, 21(1):23. DOI:10.1186/s12876-020-01593-5 .
9	吴斌, 朱宇均, 黄金路. 从"气"论治法对便秘型肠易激综合征的临床疗效及对部分脑肠肽的影响[J]. 中国医药科学, 2022, 12(11):9-13, 26.
	WU B, ZHU Y J, HUANG J L. Clinical efficacy of the Qi in treating constipation-predominant irritable bowel syndrome and its effect on brain-gut peptides[J]. China Med Pharm, 2022, 12(11):9-13, 26.
10	彭丽华, 杨云生, 孙刚, 等. 便秘型肠易激综合征新概念模型的建立[J]. 世界华人消化杂志, 2004, 12(1): 112-116.
	PENG L H, YANG Y S, SUN G, et al. A new model of constipation-predominant irritable bowel syndrome in rats[J]. World Chin J Dig, 2004, 12(1): 112-116.
11	QUIGLEY E M M. Microbiota-brain-gut axis and neuro-degenerative diseases[J]. Curr Neurol Neurosci Rep, 2017, 17(12):94. DOI:10.1007/s11910-017-0802-6 .
12	SPIELMAN L J, GIBSON D L, KLEGERIS A. Unhealthy gut, unhealthy brain: the role of the intestinal microbiota in neurodegenerative diseases[J]. Neurochem Int, 2018, 120:149-163. DOI:10.1016/j.neuint.2018.08.005 .
13	VERMEULEN W, DE MAN J G, PELCKMANS P A, et al. Neuroanatomy of lower gastrointestinal pain disorders[J]. World J Gastroenterol, 2014, 20(4):1005-1020. DOI:10.3748/wjg.v20.i4.1005 .
14	CHRISTIANSON J A, BIELEFELDT K, ALTIER C, et al. Development, plasticity and modulation of visceral afferents[J]. Brain Res Rev, 2009, 60(1):171-186. DOI:10.1016/j.brainresrev.2008.12.004 .
15	GONDA X, PETSCHNER P, ESZLARI N, et al. Effects of different stressors are modulated by different neuro-biological systems: the role of GABA-a versus CB1 receptor gene variants in anxiety and depression[J]. Front Cell Neurosci, 2019, 13: 138. DOI:10.3389/fncel.2019.00138 .
16	HOUTEPEN L C, SCHÜR R R, WIJNEN J P, et al. Acute stress effects on GABA and glutamate levels in the prefrontal cortex: A 7T ¹H magnetic resonance spectroscopy study[J]. Neuroimage Clin, 2017, 14:195-200. DOI:10.1016/j.nicl.2017. 01.001 .
17	LIBERZON I, KRSTOV M, YOUNG E A. Stress-restress: effects on ACTH and fast feedback[J]. Psychoneuroendocrinology, 1997, 22(6):443-453. DOI:10.1016/s0306-4530(97)00044-9 .
18	SANTOS J, ALONSO C, VICARIO M, et al. Neuro-pharmacology of stress-induced mucosal inflammation: implications for inflammatory bowel disease and irritable bowel syndrome[J]. Curr Mol Med, 2008, 8(4):258-273. DOI:10.2174/156652408784533788 .
19	ROBLES A, PEREZ INGLES D, MYNEEDU K, et al. Mast cells are increased in the small intestinal mucosa of patients with irritable bowel syndrome: a systematic review and meta-analysis[J]. Neurogastroenterol Motil, 2019, 31(12): e13718. DOI:10.1111/nmo.13718 .
20	YU Y C, LI J, ZHANG M X, et al. Resveratrol improves brain-gut axis by regulation of 5-HT-dependent signaling in the rat model of irritable bowel syndrome[J]. Front Cell Neurosci, 2019, 13:30. DOI:10.3389/fncel.2019.00030 .
21	刘欢, 张孟历, 于猛, 等. 醋制香附挥发油抗抑郁活性及化学成分分析[J]. 药物评价研究, 2020, 43(3):436-442. DOI:10.7501/j.issn.1674-6376.2020.03.012 .
	LIU H, ZHANG M L, YU M, et al. Antidepressant activity evaluation and GC-MS analysis of volatile oil from vinegar-made Cyperi rhizome[J]. Drug Eval Res, 2020, 43(3):436-442. DOI:10.7501/j.issn.1674-6376.2020.03.012 .
22	宋美卿, 仝立国, 贾力莉, 等. 疏肝行气对药"香橼-佛手"对抑郁大鼠行为学及海马单胺类神经递质的影响[J]. 中国药物与临床, 2021, 21(5):737-740. DOI:10.11655/zgywylc2021.05.006 .
	SONG M Q, TONG L G, JIA L L, et al. Effects of Shuxingqi medicine "Citron-bergamot" on behavior and monoamine neurotransmitters in hippocampus of depressed rats[J]. Chin Remedies Clin, 2021, 21(5):737-740. DOI:10.11655/zgywylc2021.05.006 .
23	方圆之, 高杰. 白芍的通便作用及其对便秘小鼠结肠AQP4、VIP表达的影响[J]. 山东中医杂志, 2017, 36(1):62-65. DOI:10.16295/j.cnki.0257-358x.2017.01.022 .
	FANG Y Z, GAO J. Cathartic effect of Baishao and its effect on AQP4 and VIP in mice with constipation[J]. Shandong J Tradit Chin Med, 2017, 36(1):62-65. DOI:10.16295/j.cnki.0257-358x.2017.01.022 .
24	FAN R, HUANG X, WANG Y, et al. Ethnopharmacokinetic- and activity-guided isolation of a new antidepressive compound from fructus aurantii found in the traditional Chinese medicine Chaihu-Shugan-San: a new approach and its application[J]. Evid Based Complement Alternat Med, 2012, 2012:607584. DOI:10.1155/2012/607584 .
25	李陈雪, 杨玉赫, 冷德生, 等. 枳壳化学成分及药理作用研究进展[J]. 辽宁中医药大学学报, 2019, 21(2):158-161. DOI:10.13194/j.issn.1673-842x.2019.02.043 .
	LI C X, YANG Y H, LENG D S, et al. Research progress on chemical constituents and quality evaluation of fructus aurantii[J]. J Liaoning Univ Tradit Chin Med, 2019, 21(2):158-161. DOI:10.13194/j.issn.1673-842x.2019.02.043 .

[1]	侯敏博, 崔甜甜, 苏娜瑛, 张苗苗, 焦永敏, 严建燕, 汪溪洁, 大平东子. 一例Han-Wistar大鼠垂体细胞瘤的病理学分析[J]. 实验动物与比较医学, 2023, 43(6): 654-658.
[2]	赵丽亚, 倪丽菊, 张彩勤, 汤建平, 姚养正, 聂艳艳, 顾晓雪, 赵莹. 基于多重PCR-LDR技术建立近交系大鼠单核苷酸多态性遗传检测方案[J]. 实验动物与比较医学, 2023, 43(5): 548-558.
[3]	于灵芝, 谢建芸, 冯丽萍, 魏晓锋. 金黄色葡萄球菌荧光定量PCR检测方法的建立及其在大鼠、小鼠粪便检测中的应用[J]. 实验动物与比较医学, 2023, 43(5): 566-573.
[4]	黄缨, 韦思羽, 蔡莉, 强苏静, 李冬婷, 丁玉强. 供应商来源的实验大鼠和小鼠微生物监测结果分析：以复旦大学实验动物科学部为例[J]. 实验动物与比较医学, 2023, 43(4): 347-354.
[5]	黄慧, 邓亚胜, 梁天薇, 郑艺清, 范燕萍, 荣娜, 林江. 卵巢储备功能减退动物模型的造模方法评价与分析[J]. 实验动物与比较医学, 2023, 43(4): 422-428.
[6]	夏子茵, 柴媛媛, 徐云霞, 俞沁玮, 黄鑫, 张陆勇, 江振洲. 大鼠血清尿酸液相色谱-紫外检测新方法的建立及比较研究[J]. 实验动物与比较医学, 2023, 43(3): 314-322.
[7]	谭莹, 廖文平, 高启龙, 李勇, 史新辉, 王京昆. 碘酸钠诱发视网膜色素变性大鼠模型的生理指标和组织病理学分析[J]. 实验动物与比较医学, 2023, 43(2): 124-135.
[8]	葛健, 孙静芬, 吴永杰. 牛磺酸对苯扎氯铵损伤大鼠眼角膜内皮细胞无保护作用[J]. 实验动物与比较医学, 2023, 43(1): 39-43.
[9]	许琴, 倪艳, 是文辉, 李建瑛, 刘江伟, 赵红琼, 徐新明. SPF级雄性SD大鼠回肠与结肠菌群结构比较分析[J]. 实验动物与比较医学, 2023, 43(1): 53-60.
[10]	杨丽雅, 宋涛, 何佳璘, 郭一鸣, 亓明康, 王含必, 王慧萍. 阴道萎缩大鼠模型的建立及在药效评价中的应用[J]. 实验动物与比较医学, 2022, 42(6): 531-540.
[11]	黄少凤, 林忠, 朱雪红, 刘姝岑, 宾力. 宫腔粘连大鼠模型研究进展[J]. 实验动物与比较医学, 2022, 42(6): 560-565.
[12]	高晨, 凡春玲, 李玉荣, 裴文娟, 关彩萍. 模拟高原低氧环境下运动疲劳大鼠脑皮层单羧酸转运蛋白的变化及其意义[J]. 实验动物与比较医学, 2022, 42(5): 384-392.
[13]	许冰馨, 范凯健, 王婷玉, 陈慧瑾. 地塞米松对胶原诱导性关节炎大鼠模型软骨退化的改善作用[J]. 实验动物与比较医学, 2022, 42(5): 416-422.
[14]	覃辉艳, 陈华凤, 杨慧, 罗海兰, 傅伟忠, 李庆波, 张洁宏. 缫丝水对老龄大鼠氧化应激水平的改善作用[J]. 实验动物与比较医学, 2022, 42(5): 393-400.
[15]	张笑瑞, 曹静, 吴倩倩, 刘季俊, 陈国元, 吴宝金. 不同剂型普罗布考对大鼠肠系膜淋巴转运及药代动力学的影响[J]. 实验动物与比较医学, 2022, 42(4): 275-283.

疏肝解郁方对便秘型肠易激综合征大鼠模型的疗效及脑-肠轴功能的影响

Effect of the Traditional Chinese Medicine Shuganjieyu Formula on Constipation Type Irritable Bowel Syndrome and Brain-gut Axis in Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 25

相关文章 15

编辑推荐

Metrics

本文评价