关键词: 小鼠, 肾, 移植, 动物模型

Abstract: Objective To improve the technique of kidney transplantation model in mice and to increase the success rate of operation. Methods Totally 25 pairs of C57BL/6 mice were used for kidney transplantations, and 8 C57BL/6 mice were used for control after nephrectomy. To simplify the donor kidney acquisition method, begin to separate the donor kidney from the dorsal side, reduce the separation range of the renal arteriovenous. Obtain the artery at a vascular patch. Improve the arteriovenous anastomosis technique. The donor renal artery and vein is sutured to the recipient abdominal aorta and inferior vena cava respectively by end to side anastomosis. The urethral ends of the donor were put into the recipient bladder to reconstruct the ureter, and fix the ureteral wall to the outer membrane of the bladder. The natural kidneys of the recipients were excised by two phases. Results The success rate of operation was 92%, the total operation time was 47.6±8.7 min, warm ischemia time was 3~5 s, cold ischemia time was 31.1±6.3 min. Conclusion The improvement technique of kidney transplantation model in mice can improve the success rate of operation. It is easy to learn and is worth to be widely applied.

Key words: Mice, Kidney, Transplantation, Model