关键词: 肺移植, 气管移植, 闭塞性细支气管炎, 微小miRNA, 大鼠

Abstract: Objective Exploration on simulation establishment of rat Orthotopic tracheal transplantation obliterative bronchiolitis (OB) after lung transplantation, pathophysiological process, for the study of lung transplantation in OB microRNA (miRNA) expression profiles provide an ideal animal model. Methods By Orthotopic tracheal transplantation in inbred rats, establish simulation of OB after lung transplantation animal models according to the designed groups (n=6 pairs). After successful models HE used dye to assess the tracheal transplantation model; The total RNA was extracted from transplanted trachea tissues by Trizol. Small miRNA was isolated from total RNA, hybridized on miRNA microarray, and the microarray results confirmed by quantitative real-time reverse transcription-polymerase chain reaction(RT-qPCR). Results The survival rate of established model was 86.67% (26/30) beyond four weeks. The histology suggested that the trachea endothelial cells in experiment group were damaged, accompanied by enhanced inflammatory cell infiltration and fibroproliferation. Incidence of obliterative bronchiolitis in mice of experimental group was approximately 100％. Compared with the normal control group, there were 29 specific miRNAs in the model control group with the expression difference of more than 2 times by 15 up-regulation miRNAs and 14 down-regulating miRNAs, verify which significantly increase miR-146a, miR-155, and significantly reduced miR-451 and test results with good consistency. Conclusion Stability simulation of Orthotopic tracheal transplantation OB obstructive airway disease after lung transplantation can be applied after lung transplantation animal models of miRNA expression profiles in OB research.

Key words: Lung allograft, Tracheal transplantation, Obliterative bronchiolitis, MicroRNA, Rat