II型糖尿病大鼠血糖波动模型的初步建立及其特点

doi:10.3969/j.issn.1674-5817.2012.06.012

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (6): 512-516.DOI: 10.3969/j.issn.1674-5817.2012.06.012

II型糖尿病大鼠血糖波动模型的初步建立及其特点

朱延涛, 金蓉家, 钟杰敏, 李守业, 徐志伟, 寿旗扬, 蔡月琴, 王辉

浙江中医药大学, 杭州 310053

收稿日期:2012-08-09 出版日期:2012-12-25 发布日期:2012-12-25
作者简介:朱延涛(1987-),男,在读硕士,研究方向:中药药理学,E-mail:zhuyantao297@126.com。
基金资助:
1.浙江省自然科学基金资助项目(Y2110738);2.浙江省实验动物科技计划项目(2012C37077)

Establishment of Blood Glucose Fluctuation Model on Diabetic Rats and Its Damage to Pancreas

ZHU Yan-tao, JIN Rong-jia, ZHONG Jie-min, LI Shou-ye, XU Zhi-wei, SHOU Qi-yang, CAI Yue-qin, WANG Hui

Zhejiang Chinese Medical University, Hangzhou 310053, China

Received:2012-08-09 Online:2012-12-25 Published:2012-12-25

摘要/Abstract

摘要： 目的建立II型糖尿病大鼠血糖波动模型,同时观察血糖波动对糖尿病大鼠胰腺损伤情况。方法用30 mg/kg链脲佐菌素诱导SD大鼠糖尿病模型,错时腹腔注射0.375 g/kg葡萄糖和皮下注射超短效胰岛素制备糖尿病血糖波动模型,观察各组大鼠糖化血红蛋白(HbAlc)、血清游离脂肪酸(FFA)、胰岛素(INS)、胰腺中Bax和Bcl-2含量。结果模型波动组和模型组的血糖显著高于正常组(P<0.01),模型波动组和模型组中HbAlc、FFA、INS含量显著高于正常组(P<0.01),与模型组相比,模型波动组中 HbAlc、FFA、INS含量显著高于模型组含量(P<0.01); 模型波动组胰腺中Bax含量显著升高(P<0.01)、Bcl-2含量显著下降(P<0.01)。结论每日两次错时注射胰岛素和葡萄糖可以成功建立大鼠糖尿病血糖波动模型,且血糖波动能加重胰腺损伤程度。

关键词: 2型糖尿病, 大鼠, 血糖波动模型, 胰腺损伤

Abstract: Objective To establish the animal model of diabetic rats with fluctuant high blood glucose and observe the effects of fluctuant high blood glucose on pancreas in diabetic rats. Methods The diabetic rats model was developed by injection with streptozotocin (STZ) 30 mg/kg, regularly staggered insulin 1U and glucose 0.375 mg/kg administration were used to set up blood glucose fluctuation model. General symptoms were observed and the data of FFA、INS、HbAlc in blood as well as the Bax and Bcl-2 in pancreas were measured at the thirteen week. Results (1) The typical diabetic symptoms in blood fluctuation group and diabetic group were more obvious than those in normal group. (2) The blood glucose levels in diabetic group and blood glucose fluctuation were significant higher than that in normal group. (3) The FFA、HbAlc、INS in diabetic group and blood glucose fluctuation group were higher than those in normal group (P<0.01); The FFA、HbAlc、INS in blood glucose fluctuation group was higher than those in diabetic group (P<0.01). (4) Compared with normal group, the Bax in pancreas was obviously increased (P<0.01) and the Bcl-2 was dramatically reduced in blood fluctuation group and diabetic group (P<0.01); The Bax was higher (P<0.01) and the Bcl-2 was lower (P<0.01) in blood fluctuation group than those in diabetic group. Conclusion The blood fluctuation diabetic rats model was successfully established by the method of subcutaneous insulin and intraperitoneal glucose injection in diabetic rats twice daily, and fluctuant high blood glucose aggravates the injury of the pancreas in diabetes rats.

Key words: Diabetes mellitus, Blood glucose fluctuation, Model, Pancreas

中图分类号:

Q95-33

朱延涛, 金蓉家, 钟杰敏, 李守业, 徐志伟, 寿旗扬, 蔡月琴, 王辉. II型糖尿病大鼠血糖波动模型的初步建立及其特点[J]. 实验动物与比较医学, 2012, 32(6): 512-516.

ZHU Yan-tao, JIN Rong-jia, ZHONG Jie-min, LI Shou-ye, XU Zhi-wei, SHOU Qi-yang, CAI Yue-qin, WANG Hui. Establishment of Blood Glucose Fluctuation Model on Diabetic Rats and Its Damage to Pancreas[J]. Laboratory Animal and Comparative Medicine, 2012, 32(6): 512-516.

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II型糖尿病大鼠血糖波动模型的初步建立及其特点

Establishment of Blood Glucose Fluctuation Model on Diabetic Rats and Its Damage to Pancreas

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