实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (6): 503-507.DOI: 10.3969/j.issn.1674-5817.2012.06.010

• 论著 • 上一篇    下一篇

工频电磁场接触对大鼠生殖功能的影响

郁琳, 石燕, 徐彦, 王伟锋, 苗茂华, 施惠娟, 孙兆贵, 袁伟   

  1. 上海市计划生育科学研究所, 国家人口和计划生育委员会计划生育药具重点实验室, 上海 200032
  • 收稿日期:2012-03-23 出版日期:2012-12-25 发布日期:2012-12-25
  • 作者简介:郁琳(1988-),助理实验师
  • 基金资助:
    上海市科学技术委员会项目(10140901900)

Animal Experimental Studies of Power Frequency Electromagnetic Field Exposure on Reproductive Function of Rats

YU Lin, SHI Yan, XU Yan, WANG Wei-feng, MIAO Mao-hua, SHI Hui-juan, SUN Zhao-gui, YUAN Wei   

  1. Key Laboratory of Contraceptive Drugs & Devices of NPFPC,Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China
  • Received:2012-03-23 Online:2012-12-25 Published:2012-12-25

摘要: 目的 评价工频电磁场接触对大鼠生殖功能的影响,用以研究人类的电磁场接触对生殖功能的损伤风险。方法 自行设计制备磁场发生仪,把大鼠暴露于一定强度的磁场中生活一段时间。观察雌性大鼠的甾体激素水平、动情周期和生殖状况和数量; 并观察雄性大鼠的睾丸和附睾重量、精子密度、活动精子比例和精子质膜完整性。结果 本实验发现工频电磁场强度达1 mT,每日暴露时间8 h, 自4周龄开始持续1个月,不会对雌性大鼠成年后甾体激素分泌情况和生育后代的情况产生显著影响, 仅仅导致发情频率提高; 而2 mT,每日暴露12 h,自8周龄开始持续2个月,可导致雄性大鼠的活动精子比例下降。结论 在大鼠卵母细胞和精子发生过程中,环境工频磁场会导致雌性大鼠的发情频率增加,雄性大鼠的活动精子比例下降。

关键词: 工频电磁场, 大鼠, 生育能力

Abstract: Objective An instrument of power frequency electromagnetic fields, applicable for an animal model with rats as experimental subjects, will be designed to evaluate risk of the damage on reproductive functions under low frequency electromagnetic field exposure. Method The rats were exposed to the magnetic field at certain intensity for some time. Some parameters of reproductiue function were measured including plasma levels of steroid hormones, estrous cycle, and the numbers and development status of the offspring in females, and weight of the testis and epididymis, sperm density, the proportion of motile sperms, and sperm plasma membrane integrity. Results The study found that one month exposure of 1mT electromagnetic field, 8hours daily, initiated at the age of 4 weeks didn’t induced significant change in the secretion of hormones and fertility in females, only increased estrus frequency, in the power frequency electromagnetic field at 1mT, and with daily exposure time of 8 hours, for a month since the beginning at age of 4 weeks, the grown adult female rats have not shown a significant change in the secretion of hormones and fertility to bear future generations, and only estrus frequency increased. two month exposure of 2mT electromagnetic field, 12hours daily, initiated at the age of 8 weeks induced the decline of sperm motility of males.

Key words: Frequency electromagnetic field, Rats, Fertility

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