大鼠大脑中动脉梗塞模型的同步辐射活体血管影像学研究

doi:10.3969/j.issn.1674-5817.2011.01.003

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (1): 11-15.DOI: 10.3969/j.issn.1674-5817.2011.01.003

大鼠大脑中动脉梗塞模型的同步辐射活体血管影像学研究

袁法磊¹, 管永靖^1,2, 肖体乔³, 谢红兰³, 杨国源^1,2, 王永亭¹

1.上海交通大学Med-X研究院,上海 200030;
2.上海交通大学医学院附属瑞金医院, 上海 200025;
3.中科院上海应用物理研究所, 上海 201204

收稿日期:2010-06-29 出版日期:2011-02-25 发布日期:2011-02-25
作者简介:袁法磊(1986-), 男, 在读神经生物学博士研究生, 上海交通大学Med-X研究院,E-mail: yuanfalei@gmail.com
基金资助:
国家重点基础研究发展计划(973计划)(2010CB834306); 上海市科委实验动物研究专项(课题编号 09140902400); 上海交通大学SMC-晨星青年学者奖计划

Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models

YUAN Fa-lei¹, GUAN Yong-jing^1,2, XIAO Ti-qiao³, XIE Hong-lan³, YANG Guo-yuan^1,2, WANG Yong-ting¹

1. Neuroscience and Neuroengineering Center, Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China;
2. Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China;
3. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China

Received:2010-06-29 Online:2011-02-25 Published:2011-02-25

摘要/Abstract

摘要： 目的通过同步辐射微血管造影研究大鼠大脑中动脉梗塞脑缺血模型。方法 20只300~350 g SD大鼠平均分为两组: 4-0线组和4-0线包裹硅橡胶组。施行大脑中动脉缺血手术后,立即进行同步辐射血管成像。结果单纯用4-0线组大鼠大脑中动脉没有发生完全阻塞,大脑前动脉和颈内动脉的尚有部分血供; 4-0线包裹硅橡胶组, 大鼠大脑中动脉均成功阻塞。10%大鼠具有两侧的孔型大脑中动脉(2/20只)。栓线进入深度17 mm能够有效阻塞这种异常的大鼠大脑中动脉。结论包裹硅橡胶的4-0线栓能够成功造成300~350 g大鼠大脑中动脉缺血, 单纯4-0栓线无法完全造成这一体重范围内的大鼠缺血。由于同步辐射可以进行大鼠的脑血管造影,因此可适合研究和大鼠线栓法缺血模型以及评价大鼠脑部血管变异。

关键词: 血管造影, 缺血模型, 大鼠, 同步辐射

Abstract: Objective To apply synchrotron radiation microangiography on the study of rat middle cerebral artery occlusion (MCAO) models. Method Twenty Sprague-Dawley rats weighing 300-350 g were divided into two groups: 4-0 suture group and 4-0 silicone-coated suture group. Immediately after MCAO surgery, synchrotron radiation angiography was conducted. Result The plain 4-0 nylon suture failed to completely occlude the middle cerebral artery (MCA) of the rat. There was still blood supply from anterior cerebral artery (ACA) and interior carotid artery (ICA). The silicone-coated 4-0 nylon suture successfully induced rat MCAO with 10% rats had fenestrated MCAs (2/20).The variants in these rats were occluded when the suture depth was 17 mm. Conclusion The silicone-coated 4-0 nylone suture successfully induced MCAO in rats weighing from 300 to 350 g, and the plain 4-0 suture was not able to induce complete ischemia in these rats. Synchrotron radiation microagiography is useful for studying rat MCAO models and evaluating rat cerebral abnormalities.

Key words: Angiography, Ischemic models, Rat, Synchrotron radiation

中图分类号:

Q95-33

袁法磊, 管永靖, 肖体乔, 谢红兰, 杨国源, 王永亭. 大鼠大脑中动脉梗塞模型的同步辐射活体血管影像学研究[J]. 实验动物与比较医学, 2011, 31(1): 11-15.

YUAN Fa-lei, GUAN Yong-jing, XIAO Ti-qiao, XIE Hong-lan, YANG Guo-yuan, WANG Yong-ting. Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models[J]. Laboratory Animal and Comparative Medicine, 2011, 31(1): 11-15.

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大鼠大脑中动脉梗塞模型的同步辐射活体血管影像学研究

Application of Synchrotron Radiation Microangiography on Rat Middle Cerebral Artery Occlusion Models

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