实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (6): 486-489.DOI: 10.3969/j.issn.1674-5817.2014.06.011

• 论著 • 上一篇    下一篇

不同固定液对大鼠肝脏组织石蜡切片质量的影响

赵玉琼, 陈华   

  1. 解放军总医院医学实验动物中心, 北京 100853
  • 收稿日期:2014-06-16 出版日期:2014-12-25 发布日期:2014-12-25
  • 作者简介:赵玉琼(1983-), 女, 兽医学硕士, E-mail:zhaoyuqiong@163.com

Comparison on Rat Liver Tissue Slides Embedded by Paraffin among Three Different Fixatives

ZHAO Yu-qiong, CHEN Hua   

  1. Animal Experimental Center of PLA General Hospital, Beijing 100853, China
  • Received:2014-06-16 Online:2014-12-25 Published:2014-12-25

摘要: 目的 探讨不同固定液对大鼠肝脏组织石蜡切片HE染色效果。方法 应用SD大鼠肝脏组织分别在动物空腹和空腹条件下, 选择三种常规的固定液: 体积分数10%甲醛固定液、Bouin's固定液和改良Davidson's固定液, 对肝脏组织进行固定、石蜡包埋、切片和HE染色, 观察动物空腹与未空腹条件下组织固定对切片质量的影响, 以及不同固定液对肝脏组织切片质量的影响。结果 在未空腹的条件下, 肝脏组织结构显示不清晰, 呈水样变性的形态改变; Bouin's固定液和改良Davidson's固定液固定的组织结构更清晰, 但是切片色泽不如体积分数10%甲醛固定液的鲜亮。结论 大鼠是否处于空腹状态对肝脏组织结构影响很大, 三种固定方法对大鼠肝脏组织切片质量影响差异不大。

关键词: 大鼠, 肝脏, 组织固定

Abstract: Objective To investigate the difference of three kinds of conventional fixatives on rat liver tissue slides embedded by paraffin and stained with HE. Methods Three kinds conventional fixatives, 10% neutral buffered formalin, Bouin's solution and modified Davidson's solution, were used to fix rat liver tissues with the animals in fasting or non-fasting conditions. The fixed tissues were embedded by paraffin, sliced and stained with HE. The qualities of the tissue slice were compared among different fixatives fixed under different conditions (fasting or non-fasting). Results Hydropic degeneration was observed in non-fasting rat liver tissue, and the liver tissue structure was fuzzy. The slices fixed by Bouin's solution and modified Davidson's solution showed clearer tissue structures, but not as brightly as the slices fixed by 10% neutral buffered formalin fixatives. Conclusion The conditions of fasting or non-fasting affect the liver tissue structure greatly. However, there is no significant difference among three kinds of fixatives fixed slices.

Key words: Rat, Liver, Tissue fixation

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