吲哚胺2,3双加氧酶在系统性近平滑念珠菌感染小鼠中的作用

doi:10.3969/j.issn.1674-5817.2014.04.006

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (4): 288-293.DOI: 10.3969/j.issn.1674-5817.2014.04.006

吲哚胺2,3双加氧酶在系统性近平滑念珠菌感染小鼠中的作用

吴玉娥¹, 张钰¹, 龚宝勇¹, 陈梅玲¹, 吴瑞可¹, 贾欢欢¹, 罗挺¹, 高翔², 黄韧¹

1.广东省实验动物监测所、广东省实验动物重点实验室,广州 510663;
2.广东医学院药理教研室,湛江 510265

收稿日期:2014-01-27 出版日期:2014-08-25 发布日期:2014-08-25
作者简介:吴玉娥(1978-),女,助理研究员,E-mail: jadejoanjadejoan@163.com
基金资助:
广东省自然科学基金项目(No.10151026005000-000),广东省科技计划项目(No.2010B060500-019)资助

The Role of Indoleamine 2,3-dioxygenase in Candida Parapsilosis Systemic Infectet Mice

WU Yu-e¹, ZHANG Yu¹, GONG Bao-yong¹, CHEN Meiling¹, WU Rui-ke¹, JIA Huan-huan¹, LUO Ting¹, GAO Xiang², HUANG Ren¹

1.Guangdong Laboratory Animals Monitoring Institute, Guangdong Key Laboratory of Laboratory Animals, Guangzhou 510663, China;
2. Dept. of Pharmacology, Guangdong Medical College, Zhanjiang 524023, China

Received:2014-01-27 Online:2014-08-25 Published:2014-08-25

摘要/Abstract

摘要： 目的观察吲哚胺2,3双加氧酶(IDO)在系统性近平滑念珠菌(CP)感染小鼠中的作用。方法 SPF级雌性ICR小鼠分为3组,模型组和抑制组小鼠尾静脉接种CP建立系统性近平滑念珠菌感染小鼠模型,对照组小鼠接种生理盐水。抑制组在接种CP当日经口给IDO抑制剂1甲基色氨酸(1-MT),连续给药7 d,模型组和对照组小鼠给生理盐水。分别在接菌后3 d、6 d和9 d每组小鼠施行安死术5只,采集肾脏,进行IDO、炎症相关细胞因子检测,载菌量测定和组织病理学观察。结果小鼠感染CP后肾组织IDO相对表达量升高,给予1-MT后IDO相对表达量下降;模型组肾脏IFN-γ、TNF-α、IL-6、IL-1β表达水平上调,抑制组除IFN-γ、TNF-α、IL-6和IL-1β表达水平上调外,接种CP后6 d肾脏IL-17表达水平显著升高;载菌量结果显示,与模型组比较,接菌后6 d抑制组肾脏载菌量显著高于模型组;病理观察结果显示,抑制组肾脏真菌感染病灶数量增多,组织破坏严重。结论小鼠感染CP后肾组织IDO相对表达量升高,给予IDO抑制剂1-MT后肾组织IL-17表达水平升高,促进了慢性炎症,影响了真菌清除。

关键词: 近平滑念珠菌(CP), 吲哚胺2,3双加氧酶(IDO), 小鼠

Abstract: Objective To investigate the role of indoleamine 2,3-dioxygenase (IDO) in mice model of candida parapsilosis(CP) systemic infection. Methods The female ICR mice (SPF) were randomly divided into three groups, Group A and Group B were infected with CP by tail vein injection after immunosuppression, control group were injected physiological saline by tail vein injection. Group B were given methyl tryptophan (1-MT) by oral gavage, which is the inhibitor of IDO, each a day from 0 day to 7 days after infection. While group A and control group were given physiological saline by oral gavage. Five mice were euthanatized at 3, 6, 9 days after infection and the kidney tissues were collected and examined by the expression of IDO and inflammation related cytokines, fungal burden, and histology. Results After infection CP, the level of IDO in Group A was increased, while the IDO level was went down in group B. The level of IFN-γ, NF-α, IL-6 and IL-1β in kidney was increased both in group A and group B comparing with control group. At 6 days after infection, the level of IL-17 in kidney in group B was increased significantly comparing with that of group A and control group. At 6 days after infection, the level of fungal burden in group B were higher than that of group A. More fungal infection focus and tissue destruction were seen in the kidneys under the microscope in group B. Conclusion The expression level of IDO was increased in the mice model of CP systemic infection. When the inhibitor of IDO were given to the CP mice, the level of IL-17 in kidney was increased, which might promote chronic inflammation and inhibit the clearing of fungi.

Key words: Candida parapsilosis(CP), Indoleamine 2,3-dioxygenase (IDO), Mice

中图分类号:

Q95-33

吴玉娥, 张钰, 龚宝勇, 陈梅玲, 吴瑞可, 贾欢欢, 罗挺, 高翔, 黄韧. 吲哚胺2,3双加氧酶在系统性近平滑念珠菌感染小鼠中的作用[J]. 实验动物与比较医学, 2014, 34(4): 288-293.

WU Yu-e, ZHANG Yu, GONG Bao-yong, CHEN Meiling, WU Rui-ke, JIA Huan-huan, LUO Ting, GAO Xiang, HUANG Ren. The Role of Indoleamine 2,3-dioxygenase in Candida Parapsilosis Systemic Infectet Mice[J]. Laboratory Animal and Comparative Medicine, 2014, 34(4): 288-293.

参考文献

[1] Pfaller MA, Moet GJ, Messer SA, et al.Candida bloodstream infections: comparison of species distributions and antifungal resistance patterns in communityonset and nosocomial isolates in the SENTRY Antimicrobial Surveillance Program, 2008-2009[J]. Antimicrob Agents Chemother, 2011, 55(2):561-566.
[2] Lark RL, Chenoweth C, Saint S, et al.Four year prospective evaluation of nosocomial bacteremia: epidemiology, microbiology, and patient outcome[J]. Diagn Microbiol Infect Dis, 2000, 38(3):131-140.
[3] Gudlaugsson O, Gillespie S, Lee K, et al.Attributable mortality of nosocomial candidemia, revisited[J]. Clin Infect Dis, 2003,37(9):1172-1177.
[4] Trofa D, Gacser A, Nosanchuk JD.Candida parapsilosis, an emerging fungal pathogen[J]. Clin Microbiol Rev,2008,21(4):606-625.
[5] van Asbeck EC, Clemons KV, Stevens DA. Candida parapsilosis: a review of its epidemiology, pathogenesis, clinical aspects, typing and antimicrobial susceptibility[J]. Crit Rev Microbiol, 2009, 35(4):283-309.
[6] Munn DH, Mellor AL.Indoleamine 2,3-dioxygenase and tumor induced tolerance[J].J Clin Invest, 2007, 117(5):1147-1154.
[7] Fallarino F, Grohmann C,Vacca R, et al.T cell apoptosis by tryptophan catabolism[J].Cell Death Differ, 2002, 9(10):1069-1077.
[8] Bozza S, Fallarino F, Pitzurra L, ey al. A crucial role for tryptophan catabolism at the host/Candida albieans interface[J]. J Immunol, 2005, 174(5):2910-2918.
[9] Montagnoli C, Fallarino F, Gaziano R, ey al. Immunity an d tolerance to Aspergillus involve functionally distinct regulatory T cells an d tryptophan catabolism[J]. J Immunol, 2006,176(3):1712-1723.
[10] 李哲萍, 刘小丽, 武希润, 等. FOLFOX4联合1-MT对胃癌小鼠皮下移植瘤的抑制作用[J]. 癌变·畸变·突变, 2013, 25(4):267-271.
[11] 欧雪玲, 梁伟英, 杜军, 等. 口服吲哚胺特异抑制剂1-MT治疗Lewis移植性肿瘤的实验研究[J]. 解剖学研究, 2008,30(5):347-350.
[12] Hairz U, Jurgens B, Heitger A.The role of indoleamine 2,3-dioxygenase in transplantation[J].Transpl Int, 2007, 20(2):118-127.
[13] 张防正, 胡丽华. 吲哚胺2, 3双加氧酶的免疫调节功能研究新进展[J].中国免疫学杂志, 2011, 27(1):93-95.
[14] Mackler AM, Barber EM, Takikawa O, et al.Indoleamine 2, 3-dioxygenase is regulated by IFN-gamma in the mouse placenta during Listeria monocytogenes infect ion[J]. J Immunol,2003, 170(2):823-830.
[15] Shirey KA1, Jung JY, Maeder GS, et al. Upregulation of IFN-gamma receptor expression by proinflammatory cytokines influences IDO activation in epithelial cells[J]. J Interferon Cytokine Res, 2006, 26(1):53-62.
[16] Romani L, Fallarino F, De Luca A, et al . Defective tryptophan catabolism underlies inflammation in mouse chronic granulomatous disease[J]. Nature, 2008, 451(7175):211-215.
[17] 荣令, 李家树, 周新. Th17 和Treg 在真菌感染中的研究进展[J]. 中国感染与化疗杂志, 2010, 10(1):63-67.

吲哚胺2,3双加氧酶在系统性近平滑念珠菌感染小鼠中的作用

The Role of Indoleamine 2,3-dioxygenase in Candida Parapsilosis Systemic Infectet Mice

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘欣, 石少波, 张翠, 杨波, 曲川. 小鼠自体动静脉内瘘端侧吻合模型的建立与评价[J]. 实验动物与比较医学, 2023, 43(6): 595-603.
[2]	王丹, 张晓璐, 王妍, 傅博, 王文栋, 刘京, 张甦寅, 武怡荷, 吴德国, 杜小燕, 战大伟, 章秀林, 李长龙. Big-BALB/c小鼠亚系选育前后的抗体制备效率比较研究[J]. 实验动物与比较医学, 2023, 43(6): 612-618.
[3]	聂永强, 王朝霞. 濒危基因编辑小鼠品系拯救技术及其应用探讨[J]. 实验动物与比较医学, 2023, 43(6): 636-640.
[4]	于灵芝, 谢建芸, 冯丽萍, 魏晓锋. 金黄色葡萄球菌荧光定量PCR检测方法的建立及其在大鼠、小鼠粪便检测中的应用[J]. 实验动物与比较医学, 2023, 43(5): 566-573.
[5]	王成稷, 王珏, 王海杰, 陆炜晟, 史岩, 顾正页, 万鸣秋, 沈如凌. 乳胶血管灌注技术制作小鼠头面部静脉血管模型方法初探[J]. 实验动物与比较医学, 2023, 43(5): 574-578.
[6]	翟珊珊, 梁亮, 曹颖颖, 李竹欣, 王青, 陶俊宇, 运晨霞, 冷静, 唐海波. 一例树鼩毛发上皮瘤的诊断及细胞生物学特性观察[J]. 实验动物与比较医学, 2023, 43(4): 440-445.
[7]	黄缨, 韦思羽, 蔡莉, 强苏静, 李冬婷, 丁玉强. 供应商来源的实验大鼠和小鼠微生物监测结果分析：以复旦大学实验动物科学部为例[J]. 实验动物与比较医学, 2023, 43(4): 347-354.
[8]	谈小倩, 杨颢, 唐慧青, 瞿伟, 李亮, 钱珍, 顾坚忠, 徐平, 肖君华. BALB/cA.Cg.SHJH ^hr 小鼠的培育及其相关遗传学特性分析[J]. 实验动物与比较医学, 2023, 43(4): 363-370.
[9]	邓亚胜, 林江, 甘池伶, 曾官凤, 黄嘉茵, 邓慧芳, 麻颖贤, 韩丝银. 皮肤光老化动物模型制备要素和受试物数据的文献分析[J]. 实验动物与比较医学, 2023, 43(4): 406-414.
[10]	王雪, 呼永河. 糖尿病小鼠模型的常见种类及其构建要素分析[J]. 实验动物与比较医学, 2023, 43(4): 415-421.
[11]	黄慧, 邓亚胜, 梁天薇, 郑艺清, 范燕萍, 荣娜, 林江. 卵巢储备功能减退动物模型的造模方法评价与分析[J]. 实验动物与比较医学, 2023, 43(4): 422-428.
[12]	潘志强, 农淄心, 谢海纳, 彭佩克. 顺铂对小鼠下丘脑-垂体-肾上腺/性腺轴功能的损伤作用及脱氢表雄酮的干预效应[J]. 实验动物与比较医学, 2023, 43(3): 229-242.
[13]	郭文文, 赵亚, 王颖花, 刘可, 葛煦, 张延英, 汪永锋, 师长宏. 人参皂苷Rg1在小鼠创伤性脑损伤修复中的作用[J]. 实验动物与比较医学, 2023, 43(3): 243-252.
[14]	张渊, 李晗, 张成芳. 奥氮平诱导体重增加小鼠的全脑转录组学分析[J]. 实验动物与比较医学, 2023, 43(3): 262-270.
[15]	谭志刚, 刘锦信, 郑楚雅, 廖文峰, 冯露平, 彭红丽, 严秀, 卓振建. 神经母细胞瘤动物模型研究进展与应用[J]. 实验动物与比较医学, 2023, 43(3): 288-296.