小鼠股动脉及胃插管复合手术模型制备方法

doi:10.3969/j.issn.1674-5817.2013.02.012

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (2): 139-143.DOI: 10.3969/j.issn.1674-5817.2013.02.012

小鼠股动脉及胃插管复合手术模型制备方法

岳霞, Ruth Magaye, 王尊, 戴娜, 赵进顺, 邹宝波

宁波大学医学院, 宁波 315211

收稿日期:2012-07-17 出版日期:2013-04-25 发布日期:2013-04-25
作者简介:岳霞(1988-), 女, 硕士研究生,E-mail: xiayue324@163.com
基金资助:
宁波大学实验技术项目(SYJS-201101), 宁波市科研项目(SZX11073)

Introduction of a Micro-Surgery Model through Femoral Arterial Catheters and Gastric Cannula in Mice

YUE Xia, Ruth Magaye, WANG Zun, DAI Na, ZHAO Jin-shun, ZOU Bao-bo

Medical School of Ningbo University, Ningbo 315211, China

Received:2012-07-17 Online:2013-04-25 Published:2013-04-25

摘要/Abstract

摘要： 目的介绍一种小鼠经股动脉及胃插管制作的复合手术模型。方法雄性小鼠异氟烷麻醉后,先放置股动脉导管。然后,在左上腹行横向切口,将已经准备好的胃插管植入左上腹处皮下,并从颈部引出。切开腹部肌肉,暴露胃体,在胃的底部造口后植入胃插管。固定双导管,将其通过旋转输液阀与输液泵相连,术后输液泵持续灌注20 u/ml的肝素(12~15 µl/h)。结果手术后1~2 d,动物基本都能恢复到正常生理状况。手术后7 d,动脉导管与胃管均无堵塞且固定良好,无伤口感染、胃瘘管及腹腔脓肿。结论本文介绍的这种在非禁食状态下,经显微外科手术建立的小鼠股动脉及胃插管动物模型,手术后的动物损伤轻,外来异物在小鼠胃内占的比例较小,有利于动物的恢复与进食消化。这是一种动态、安全有效的手术模型,可广泛用于营养代谢、药理学、毒理学等领域的实验研究。

关键词: 小鼠, 股动脉插管, 胃插管, 显微外科手术模型

Abstract: Objective A micro-surgery model through femoral arterial and gastric cannula in mice is introduced. Methods After mice were anesthetized by inhalation of isoflurane, catheters were placed into the femoral artery. Thereafter, gastric cannula was tunneled subcutaneously through two incisions between the back of the neck and the left upper quadrant. After cutting through the abdominal muscle, the stomach was exposed. Gastric cannula was then implanted into the lower end of the stomach through an incision on the gastric wall. Heparin (20 u,12~15 µl/h) were continuously infused in both catheters through a double-channel swivel connected with a pump. Result All mice fully recovered after surgeryŁ¨1ˇ«2 daysŁ©. The artery catheters and gastric cannula work well until 7 day after micro-surgery operation. Wound infection, gastric fistula and peritoneal abscess were not found. Conclusion This is a dynamic, safe, as well as efficient micro-surgery model, with less injury to the mice and smaller foreign matter in the mouse stomach. It will be a useful model for pharmacological, toxicological, and clinical experimental studies.

Key words: Mice, Femoral artery catheter, Gastric cannula, Micro-surgery model

中图分类号:

R332Q95-33

岳霞, Ruth Magaye, 王尊, 戴娜, 赵进顺, 邹宝波. 小鼠股动脉及胃插管复合手术模型制备方法[J]. 实验动物与比较医学, 2013, 33(2): 139-143.

YUE Xia, Ruth Magaye, WANG Zun, DAI Na, ZHAO Jin-shun, ZOU Bao-bo. Introduction of a Micro-Surgery Model through Femoral Arterial Catheters and Gastric Cannula in Mice[J]. Laboratory Animal and Comparative Medicine, 2013, 33(2): 139-143.

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小鼠股动脉及胃插管复合手术模型制备方法

Introduction of a Micro-Surgery Model through Femoral Arterial Catheters and Gastric Cannula in Mice

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