关键词: 小鼠, 吗啡成瘾, 甘珀酸, 条件性位置偏爱(CPP)

Abstract: Objective To investigate the effect of gap juction inhibitor carbenoxolone (CBX) on morphine addiction in mice. Methods Conditioned place preference (CPP) was established in ICR mice through prolonged morphine exposure. The influence of CBX on the acquisition or expression of CPP were observed through behavior test. Changes of the astrocytes were examined by immunofluorescence of glial fibrillary acidic protein (GFAP). Results ①Contrast to the control group, CPP was induced in the morphine-dependent group (P<0.05). ②CPP was inhibited significantly in the CBX-pretreated group(P<0.05), but not significantly in the CBX-treated group (P>0.05). The morphine may lead to increase of GFAP immunostaining in ventral tegmental area (VTA). ③The GFAP immuno-reactive cells in CBX-pretreated group were fewer than those in morphine group. Conclusion CBX can suppress the acquisition of CPP induced by morphine, but the effect of CBX on the express of CPP was weak. CBX maybe play directly or by indirect effect of GFAP.

Key words: Mice, Morphine addiction, Carbenoxolone, Conditioned place preference (CPP)