›› 1993, Vol. 13 ›› Issue (4): 200-202.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

近交系小鼠超数排卵的研究

  

  1. 中国人民解放军军事医科学院实验动物中心
  • 收稿日期:1993-07-25 出版日期:1993-01-25 发布日期:2013-03-21

Study on Superovulation in Inbred Strain Mice

  1. Laboratory Animal Center,Academy of Military Medical Sciences,Beijing 100071
  • Received:1993-07-25 Online:1993-01-25 Published:2013-03-21

摘要: 近交系小鼠超数排卵(简称超排)的研究可为小鼠胚胎库的建立提供大量胚胎。以孕马血清促性腺激素(PMSG)、人绒毛膜促性腺激素(HCG)为主,按不同品系小鼠的特点,加前列腺素(PGF)1μg/只以上。超排BALB/c系小鼠,平均每只雌鼠取可用胚胎22枚,C57BL/6;A系小鼠为23枚,对照组分别取得7枚和8枚,实验组与对照组差异显著(P<0.05)。超排处理后的雌鼠交配受孕率较高。实验结果提示改进的超排方法可提高国产激素制品的超排效果。

关键词: 近交系, 小鼠, 激素, 超数排卵

Abstract: Super ovulation in inbred mice can provide a lot of embryos for the establishment of mouse embryo cryobank.Pregnant mare serum gonadotropin and human chorionic gonadotropin were used for the superovulation in BALB/c and C 57 BL/6 A mice, satisfactory results were obtained by the addition of more than 1 μg of 15-methyl prostaglandin F according to the characteristics of the mouse strains. The results show that the average usable embryos obtained from one superovulated BALB/c and C57BL/6 A mouse are 22 and 23 respectively, but only 7 and 8 in control animals. The pregnancy rate is higher in the treated mice than the controls.

Key words: Inbredsnmice, Hormone, Superovulation