实验动物与比较医学 ›› 1993, Vol. 13 ›› Issue (4): 200-202.
• 论文 •
摘要： 近交系小鼠超数排卵（简称超排）的研究可为小鼠胚胎库的建立提供大量胚胎。以孕马血清促性腺激素（PMSG）、人绒毛膜促性腺激素（HCG）为主，按不同品系小鼠的特点，加前列腺素（PGF2α）1μg/只以上。超排BALB/c系小鼠，平均每只雌鼠取可用胚胎22枚，C57BL/6；A系小鼠为23枚，对照组分别取得7枚和8枚，实验组与对照组差异显著（P＜0.05）。超排处理后的雌鼠交配受孕率较高。实验结果提示改进的超排方法可提高国产激素制品的超排效果。
Abstract: Super ovulation in inbred mice can provide a lot of embryos for the establishment of mouse embryo cryobank.Pregnant mare serum gonadotropin and human chorionic gonadotropin were used for the superovulation in BALB/c and C 57 BL/6 A mice, satisfactory results were obtained by the addition of more than 1 μg of 15-methyl prostaglandin F2α according to the characteristics of the mouse strains. The results show that the average usable embryos obtained from one superovulated BALB/c and C57BL/6 A mouse are 22 and 23 respectively, but only 7 and 8 in control animals. The pregnancy rate is higher in the treated mice than the controls.
. Study on Superovulation in Inbred Strain Mice[J]. Laboratory Animal and Comparative Medicine, 1993, 13(4): 200-202.
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