小鼠脊髓损伤打击模型精细行为的比较研究

doi:10.12300/j.issn.1674-5817.2020.221

实验动物与比较医学 ›› 2021, Vol. 41 ›› Issue (4): 290-298.DOI: 10.12300/j.issn.1674-5817.2020.221

小鼠脊髓损伤打击模型精细行为的比较研究

颜义彤^1,2,3,4, 白帆^1,2,3,4, 荆瀛黎^1,2,3,4, 王立淼^1,2,3,4, 李子晗^1,2,3,4, 于艳^1,2,3,4

1.中国康复科学所, 北京 100068;
2.首都医科大学康复医学院, 北京 100068;
3.北京脑重大疾病研究院神经损伤与修复研究所, 北京 100068;
4.神经损伤与康复北京市重点实验室, 北京 100068

收稿日期:2020-12-24 修回日期:2021-02-02 发布日期:2021-08-30
作者简介:颜义彤(1994—), 女, 硕士, 研究实习员, 研究方向为神经生理学。E-mail: yitongyan94@163.com。于艳,神经生物学博士,现任中国康复科学所康复医学研究所所长。主要研究方向为中枢神经系统损伤修复及康复治疗技术,围绕脑损伤康复及脑可塑性、脊髓损伤的再生及免疫细胞治疗、神经损伤并发症的治疗,结合电化学、基因组学、代谢组学、生物信息学等手段,解决康复医学基础研究中的重大问题。参与组建中国康复医学所神经科学研究与发现中心、北京脑重大疾病研究院神经损伤与修复研究所和神经损伤与康复北京市重点实验室等康复基础及临床研究基地。以第一作者或通信作者在Microbiome 、Oxidative Medicine and Cellular 等期刊发表科研论文十余篇。获得发明专利及实用新型专利等7项。获得北京医学会科技进步奖和中国康复医学会科技进步奖各1项。
基金资助:
国家自然科学基金资助项目(82071400); 中央级公益性科研院所基本科研业务费专项资金项目(2020CZ-2; 2020CZ-13)

A Comparative Study on Spontaneous Homecage Behaviors of Spinal Cord Injury Mice

YAN Yitong^1,2,3,4, BAI Fan^1,2,3,4, JING Yingli^1,2,3,4, WANG Limiao^1,2,3,4, LI Zihan^1,2,3,4, YU Yan^1,2,3,4

1. China Rehabilitation Science Institute, Beijing 100068, China;
2. School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China;
3. Center of Neural Injury and Repair, Beijing Institute of Major Brain Disorders, Beijing 100068, China;
4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, China

Received:2020-12-24 Revised:2021-02-02 Published:2021-08-30

摘要/Abstract

摘要： 目的探讨脊髓损伤小鼠除运动功能障碍外,在自主运动、探索行为和自我照顾行为等家笼（homecage）内精细行为方面的异常表现。方法 8周龄雌性C57BL/6小鼠10只,接受T10脊髓打击造模。另选11只小鼠为假手术组。术后1~5周,每日固定时间将小鼠置于家笼中记录其精细行为,使用HomeCageScan软件分析动物32种日常行为所占时间百分比。结果与假手术组动物相比,早期脊髓损伤小鼠的探索行为、自主运动和自我照顾行为中多数行为活动均存在显著异常（P<0.01）,部分行为在损伤后1~5周出现恢复。脊髓损伤后1~5周小鼠以直立和嗅为代表的探索行为与假手术组相比明显减少（P<0.01）,分别于术后第4周与第3周恢复至与术后第1周有明显差异（P<0.05）;脊髓损伤组小鼠以缓慢行走和跳跃为代表的自主运动与假手术组相比明显减少（P<0.05）,分别于术后第2周和第5周恢复至与术后第1周有明显差异（P<0.05）;以抽搐和理毛行为为代表的自我照顾行为与假手术组相比明显增加（P<0.01）,且在术后1~5周无明显改善。结论脊髓损伤小鼠除双下肢运动功能障碍外,还存在探索行为、自主运动和自我照顾行为等方面的异常。损伤后1~5周,探索行为和自主运动行为出现不同程度的恢复,而自我照顾行为持续异常。自动化高通量家笼内精细行为检测系统可对数十种动物行为进行精确分类和全面记录,是针对脊髓损伤行为障碍的一种更全面和敏感的评价工具。

关键词: 脊髓损伤, 行为学, 家笼内精细行为, 小鼠

Abstract: Objective To investigate the dysfunction of spontaneous homecage behaviors in mice with spinal cord injury, including exploratory behaviors, voluntary movements, and self-care behaviors. Methods Eight-week female C57BL/6 mice (n = 10) were established into thoracic 10 contusion spinal cord injury models. Other mice (n = 11) were included in the sham group. From the first week to the fifth week after surgery, the mice were observed in homecages at 9 a.m. for 2 hours once a week. The HomeCageScan software was used to analyze the time percentages of 32 daily behaviors. Results Compared with the sham group, the spinal cord injury mice showed significant abnormalities in most aspects of daily behaviors, such as exploratory behaviors, voluntary movements, and self-care behaviors (P < 0.01). Some of these behaviors recovered over time after surgery. At 1-5 weeks after surgery, the spinal cord injury mice exhibited less exploratory behaviors than the sham mice, represented by rearing up and sniffing (P < 0.01), and showed recovery in the 4th week and the 3rd week (P < 0.05). The spinal cord injury mice also exhibited less voluntary movements (P < 0.05), represented by walking slowly and jumping, and showed recovery from the 2nd week and the 5th week, respectively (P < 0.05). The spinal cord injury mice exhibited more self-care behaviors than the sham mice (P < 0.01), represented by twitching and grooming, and showed no significant recovery over time. Conclusion Besides lower limb movement dysfunction, the spinal cord injury mice showed abnormalities in exploratory behaviors, voluntary movements, and self-care behaviors. From 1-5 weeks after injury, the exploratory behaviors and voluntary movements could show different degrees of recovery, while the self-care behaviors showed continuous abnormalities. The automated high-throughput behavior detection system accurately classified and comprehensively recognized dozens of behaviors, which could be used as a more comprehensive and sensitive evaluation index of spinal cord injury disorder.

Key words: Spinal cord Injury, Ethology, Homecage behaviors, Mice

中图分类号:

Q95-33
R-332

颜义彤, 白帆, 荆瀛黎, 王立淼, 李子晗, 于艳. 小鼠脊髓损伤打击模型精细行为的比较研究[J]. 实验动物与比较医学, 2021, 41(4): 290-298.

YAN Yitong, BAI Fan, JING Yingli, WANG Limiao, LI Zihan, YU Yan. A Comparative Study on Spontaneous Homecage Behaviors of Spinal Cord Injury Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(4): 290-298.

图/表 7

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小鼠脊髓损伤打击模型精细行为的比较研究

A Comparative Study on Spontaneous Homecage Behaviors of Spinal Cord Injury Mice

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