小动物断层扫描系统在小鼠爆发性肝损伤模型中的应用

doi:10.3969/j.issn.1674-5817.2016.03.004

摘要/Abstract

摘要： 目的利用小动物断层扫描(MicroCT)系统观察内毒素联合D-氨基半乳糖(LPS/D-GalN)所致小鼠爆发性肝损伤模型中的影像学表现和CT值变化,并分析影像学表现与小鼠肝损伤之间的关系及机制。方法取5只雄性C57BL/6小鼠进行爆发性肝损伤造模(LPS 10 μg/kg+D-GalN 700 mg/kg,腹腔注射),分别于造模前,造模后3 h、6 h利用MicroCT行小鼠活体肝组织扫描,根据扫描图像所获得资料测量小鼠肝脏的平均CT值,分析比较各组肝损伤征象的显示率差异。同时,取20只小鼠分为4组,造影剂(ExiTron nano 6000)组,给予造影剂注射,检测造影剂对肝脏有无毒性; PBS组, 给予同造模组相同剂量的PBS注射; 造模3 h组,肝损伤造模后3 h取血和组织标本; 造模6 h组,造模后6 h取标本。然后比较MicroCT显像与小鼠血液及病理结果的相关性。结果以ExiTron nano 6000 做为造影剂, MicroCT可清晰的显影肝脏和脾脏的二维和三维结构。LPS/D-GalN可以造成严重的肝脏损伤。给予LPS/D-GalN处理后3 h,小鼠肝脏的CT值明显升高,体外实验证明LPS可增强巨噬细胞吸收造影剂的能力。结论 MicroCT可通过无创方法在小鼠爆发性肝损伤早期反应肝脏损伤的变化,具有较高的应用前景。

关键词: 爆发性肝损伤, 小动物断层扫描(Micro CT), ExiTron nano 6000, 巨噬细胞, 小鼠

Abstract: Objective To explore the potential use and mechanism of MicroCT using ExiTron nano6000 in the assessment of fulminant liver injury in mouse models. Methods Five male C57BL/6 mice were subjected to administration of LPS/D-GalN to develop model of fulminant liver injury. The mice were scanned with MicroCT before the treatment and at 3 h and 6 h after the establishment of liver injury model. The average CT value of liver were analyzed with software for future study. Twenty male mice were divided into 4 groups, 5 mice in each group: Nano 6000 group, the mice were treated with Nano 6000; PBS group, treated with PBS as control; model 3 h group and 6 h group, injected with LPS/D-GalN and sacrificed at 3 h, 6 h after the injection. Pathological damage of the liver were observed by microscope and serum ALT and AST were detected. Results MicroCT with ExiTron nano6000 as contrast agent could provided specific and clear 2 dimensions and 3 dimensions structure of liver and spleen in mice. The average CT value of liver was significantly increased at 3 h after LPS/D-GalN treatment and drop to baseline level at 6 h. In vitro experiments showed that LPS stimulation could enhance the endocytotic ability of macrophages to uptake ExiTron nano6000. Conclusions The Micro CT provided a rapid noninvasive longitudinal monitoring of fulminant liver injury in mice models and may be had good application in future research.

Key words: Fulminant liver injury, Micro CT, ExiTron nano 6000, Macrophage, Mice

中图分类号:

Q95-33

李大伟, 华相伟, 张江, 姚菊芳, 戴慧莉, 孔宪明. 小动物断层扫描系统在小鼠爆发性肝损伤模型中的应用[J]. 实验动物与比较医学, 2016, 36(3): 180-185.

LI Da-wei, HUA Xiang-wei, ZHANG Jiang, YAO Ju-fang, DAI Hui-li, Kong Xian-ming. Non-invasive Assessment of Fulminant Liver Injury with Contrast-Enhanced Micro CT in Mice Models[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 180-185.

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