关键词: 自发高脂血症, 长爪沙鼠模型, PCR-DGGE, 血清脂蛋白琼脂糖电泳

Abstract: Objective To initially establish electrophoresis detection method of spontaneous hyperlipidemia Mongolian Gerbil for the further evaluation of hyperlipidemia model. Methods The gerbils were divided into 7 groups, and anesthetized by CO₂, then blood was collected for the detection of serum transaminases, CHO (TC), TG, HDL, LDL and the liver tissues was collected for pathological observation. Serum lipoprotein electrophoresis (agarose gel electrophoresis) was conducted by SEBIA system.The ileum and caecum intestinal contents were collected and detected for the diversity of intestinal microorganisms by PCR-DGGE method. Referring to the classification criteria of human hyperlipidemia, the gerbil hyperlipidemia were typed. Results Approximately10%~30% of older gerbils (averaged 12.81 months old) were spontaneous hyperlipidemia, their serum alanine aminotransferase, aspartate aminotransferase, triglycerides, total cholesterol, blood glucose and other indexes were significantly higher than youth groups (averaged 4.72 months old) (P<0.05). Pathological examination revealed that old group had more obvious hepatic steatosis. DGGE results showed that the model group and the young group presented different band spectrum. The band number of young group were significantly more than old group. Hyperlipidemia gerbils could be divided into five types according to the serum lipids indicators and β bands, corresponded to person’s Ⅱ a, Ⅱ b, Ⅲ, Ⅳ, Ⅴ. Conclusions Spontaneous hyperlipidemia of old gerbils could be as a potential model for study, agarose electrophoresis and PCR-DGGE maybe as additional methods for evaluating hyperlipidemia gerbil models in the future.

Key words: Spontaneous hyperlipidemia, Mongolian Gerbil model, PCR-Denaturing Gradient gel Electrophoresis (PCR-DGGE), Serum lipoprotein electrophoresis