关键词: 顺铂, 肾损伤, 小鼠

Abstract: Objective To establish a model of acute renal function impairment induced by cisplatin in mice. Method The mice were injected with 10 mg/kg cisplatin in the 1st day to establish acute renal impairment model. The mice in the control group were dosing commensurable 0.9% NaCl saline solution. The mice were taken eyeballs for blood collection after anesthesia by pentobarbitalin the 8th day, and the serum CRE, BUN levels were measured. Pathological analysis was taken to compare the changes of the renal tissues between model group and control group. Results CRE and BUN levels were raised obviously in the model group, renal index, body weight and immune organic weight were reduced obviously in the model group, at the same time, the liver index was raised in the model group. The result of the pathological analysis was showed that the degeneration and necrosis of renal tubular epithelial cells were observed in the model group, and there are some inflammatory infiltrates foci in renal mesenchymal cells in the model group. Conclusion Acute renal impairment model could be established by injected 10 mg/kg cisplatin in 32-40 g mice.

Key words: Cisplatin, Acute renal impairment, Mice