一种以小鼠精子为主导的综合性保种体系

doi:10.3969/j.issn.1674-5817.2012.02.013

实验动物与比较医学 ›› 2012, Vol. 32 ›› Issue (2): 142-145.DOI: 10.3969/j.issn.1674-5817.2012.02.013

一种以小鼠精子为主导的综合性保种体系

李敦高¹, 蒋满喜², 陈学进², 李和平¹

1.东北林业大学野生动物资源学院, 哈尔滨 150040;
2.上海交通大学医学院实验动物科学部, 上海 200025

收稿日期:2011-09-28 出版日期:2012-04-25 发布日期:2012-04-25
作者简介:李敦高(1986-),男,硕士研究生,从事转基因动物研究。E-mail:ldglidungao@126.com
基金资助:
上海市科学技术委员会科研计划项目(编号: 09140901000和10140901700)

A Comprehensive Method of Strain Conservation Mediated by Spermatozoa in Mice

LI Dun-Gao¹, JIANG Man-Xi², CHEN Xue-Jin², LI He-Ping¹

1. College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China;
2. Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Received:2011-09-28 Online:2012-04-25 Published:2012-04-25

摘要/Abstract

摘要： 目的利用活体保种、精子冷冻技术及辅助生殖技术建立一套安全的综合性保种体系。方法以单侧睾丸摘除方法获得ICR、BDF1、C57BL/6J和GFP四种小鼠的附睾精子进行冷冻保存实验,术后恢复的公鼠继续用于自然繁育。如果无法自然繁育,则将冻融精子用于体外受精(IVF)。如果冻融精子仍无法受精获得后代, 则需要借助卵胞质内单精子注射技术(ICSI)进行胚胎构建, 胚胎移植, 直至获得后代。结果单侧睾摘除的BDF1、C57BL/6J的公鼠可以继续繁育; 不育的ICR和GFP小鼠的冻融精子IVF后, 只有ICR小鼠可以得到正常的胚胎和子鼠; 而以C57BL/6J为遗传背景的GFP小鼠,其冻融精子只有采用ICSI技术,才可以得到子代。结论精子冻融小鼠的遗传背景是影响IVF成功与否的关键性因素,同时还建立了一种以小鼠精子为主导的综合性保种体系,既可以达到品系保存的目的,同时又可以最大限度地利用小鼠品系。

关键词: 小鼠, 单侧睾丸摘除, 体外受精, 胞质内单精子注射, 品系保存

Abstract: Objective To establish a comprehensive system for mouse strain conservation through natural mating, sperm cryopreservation and assisted reproductive technology. Methods Mouse fresh epididymal sperm were collected by vasectomy of unilateral testis and the vasectomized mice were naturally mated for breeding. If no satisfactory results obtained from natural breeding, then the cryopreserved sperm were thawed for In Vitro Fertilization (IVF). If no results obtained, Intracytoplasmic Sperm Injection (ICSI) was used to reconstructed embryos and which were finally transferred into pseudopregnant recipient female mouse. Results The results show that parts of mice vasectomized unilateral testis such as BDF1 and C57BL/6J mice could be used for natural breeding. Among the remaining sterile males, only ICR mice could be preserved through IVF while ICSI technology was the last approach to save GFP mice with C57BL/6J background. Conclusion The genetic background of mouse sperm cryopreservation was a key factor in IVF and setup a comprehensive system, which could achieve the purpose of strain conservation while mouse strains could be utilized at maximum extent.

Key words: Mouse, Vasectomy of Unilateral Testis, In Vitro Fertilization, Intracytoplasmic Sperm Injection, Strain conservation

中图分类号:

Q95-33

李敦高, 蒋满喜, 陈学进, 李和平. 一种以小鼠精子为主导的综合性保种体系[J]. 实验动物与比较医学, 2012, 32(2): 142-145.

LI Dun-Gao, JIANG Man-Xi, CHEN Xue-Jin, LI He-Ping. A Comprehensive Method of Strain Conservation Mediated by Spermatozoa in Mice[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 142-145.

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一种以小鼠精子为主导的综合性保种体系

A Comprehensive Method of Strain Conservation Mediated by Spermatozoa in Mice

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