关键词: 主动脉弓缩窄, 左室肥厚, 小鼠

Abstract: Objective To compare difference of the function of the hypertrophic hearts induced by transverse aortic constriction(TAC) in different strain mouse with microsurgery. Methods KM and C57BL/6 mice randomly divided into three groups: before operation (BO)(n=10)、sham-operation (SH) (n=10) and transverse aortic constriction(TAC)(n=30). Echocardiographic assessments, hemodynamic determineation, organ weight measurement and histological examination were performed before and at 8 weeks after surgery, and observed the cause of death. Results The successful rate of KM and C57BL/6 TAC groups was 80% and 87%. The survival rate of two TAC groups was 67% and 77% after 8 week; Compared respectively with SH group, at 8 week after surgery, anterior wall thickness at systole and diastole (Awsth; Awdth)、posterior wall thickness at systole and diastole (Pwsth; Pwdth)、LV mass index (LVMI)、systolic blood pressure(SBP)，diastolic blood pressure (DBP)、left ventricular systolic pressure (LVSP)、left ventricular end-diastolic pressure (LVEDP)、rate of rise of left ventricular pressure (士 dp/dtmax)、the heart weight-to-body weight ratio (Hw/Bw)、left ventricular Weight(LVw)、the LV weight-to-body weight ratio (LVw/Bw) of KM and C57BL/6 TAC groups increased significantly(P＜0.05). Compared with KM SH group, the left ventricular end-systolic dimension (LVESD)、Ventricular end-diastolic dimension (LVEDd), external left ventricular diastolic diameter (EXLVDd) of KM TAC group increased markedly (P＜0.05). Compared with C57BL/6 TAC group, LVESD、LVEDd、EXLVDd、LVw、the Lung weight-to-body weight ratio(Lungw/Bw) of KM TAC group increased markedly (P＜0.05).Conclusion KM mice can establish a TAC model that induces the myocardium of LV from hypertrophy tc heart failure. The result is as the same as C57BL/6 mice’s. KM mice shows the tendency of eccentric hypertrophy early.

Key words: Transverse aortic constriction, Left ventricular hypertrophy