皮肤光老化动物模型制备要素和受试物数据的文献分析

doi:10.12300/j.issn.1674-5817.2023.026

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (4): 406-414.DOI: 10.12300/j.issn.1674-5817.2023.026

皮肤光老化动物模型制备要素和受试物数据的文献分析

邓亚胜(), 林江, 甘池伶, 曾官凤, 黄嘉茵, 邓慧芳, 麻颖贤, 韩丝银()()

广西中医药大学基础医学院, 南宁 530200

收稿日期:2023-02-24 修回日期:2023-04-11 出版日期:2023-08-25 发布日期:2023-08-25
通讯作者: 韩丝银（1991—），女，博士，副教授，研究方向：海洋药物研究及产品开发工作。E-mail：1715252639@qq.com。ORCID：0000-0001-8562-7723
作者简介:邓亚胜（1993—），男，硕士研究生，研究方向：特色方剂的配伍及成药化研究。E-mail：1440362586@qq.com
基金资助:
广西自然科学基金项目“合浦珍珠提取液对中波紫外线诱导人皮肤角质形成细胞光老化干预机制的基础研究”(2020GXNSFBA159039);广西高校中青年教师科研基本能力提升项目“水解南珠粉对人体皮肤美白及抗衰老作用机制的研究”(2019KY0312);广西中医药大学引进博士科研启动基金项目“南珠粉及水解成分的美白活性物质初探和合成筛查研究”(2018BS013);2021年国家级大学生创新创业训练计划项目“南珠粉对Ⅱ级烫伤小鼠皮肤的修复作用研究”(202110600005);2019年广西中医药大学校级科研青年基金项目“南珠提取物对光老化人皮肤细胞的干预作用研究”(2019QN003)

Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects

Yasheng DENG(), Jiang LIN, Chiling GAN, Guanfeng ZENG, Jiayin HUANG, Huifang DENG, Yingxian MA, Siyin HAN()()

School of Basic Medical Sciences, Guangxi University of Chinese Medicine, Nanning 530200, China

Received:2023-02-24 Revised:2023-04-11 Published:2023-08-25 Online:2023-08-25
Contact: HAN Siyin (ORCID: 0000-0001-8562-7723), E-mail: 1715252639@qq.com

摘要/Abstract

摘要：

目的分析皮肤光老化动物模型的造模要素和受试物情况，为该动物模型的制备和完善提供参考，也为科学评价受试物提供依据。方法通过在中国知网、万方、PubMed数据库中检索收集2010—2022年皮肤光老化动物模型制备的相关文献，对文献中记载的模型动物种类、性别、造模方法、造模周期、辐射光源与造模部位距离、累计辐射量、检测指标、受试物（药物或治疗手段）内容进行整理归纳，建立数据库后进行统计分析。结果筛选出257篇符合纳入标准的文献，其中模型动物使用最多的是SKH-1无毛小鼠，其次为SD大鼠和KM小鼠；动物的性别选择以单一雌性为主，常采用中波紫外线（ultraviolet B，UVB）作为辐射光源，辐射光源与造模部位的距离多为30 cm，造模周期多控制在40～60 d；长波紫外线（ultraviolet A，UVA）累计照射剂量在100～150 J/cm²的所占比例最大，中波紫外线（ultraviolet B，UVB）累计照射剂量在5～10 J/cm²的所占比例最大。模型建立后采用的检测指标为皮肤组织病理检查、皮肤组织匀浆、纤维染色、免疫印迹检查等。受试物包括中药、中药提取物、中成药、中药复方、化学药、生物制剂以及其他治疗手段，同时皮肤光老化动物模型还应用于中医外治、物理疗法、阳性对照药方面的临床疗效研究。结论皮肤光老化动物实验常选用SKH-1雌性无毛小鼠，采用UVB作为辐射光源，造模周期多控制在40～60 d，UVB累计照射剂量在0～10 J/cm²，按照最小红斑量（minimum erythema dose，MED）逐周递增方式进行造模，具有成模率高、重现性好以及与临床疾病高度吻合等优点。

关键词: 皮肤光老化, 动物模型, 造模要素, 受试物, SKH-1无毛小鼠, 中波紫外线

Abstract:

Objective To analyze the modeling elements and subjects of the animal model of skin photoaging, and to provide a reference for the preparation and improvement of the model and a basis for the scientific evaluation of the subject. Methods By searching and collecting relevant literature on the preparation of animal models of skin photoaging from 2010 to 2022 in the China National Knowledge Infrastructure, Wanfang Database, and PubMed database, the model animal species, gender, modeling method, modeling cycle, radiation source and its distance from the modeling site, cumulative radiation volume, detection indicators, and subjects (drugs or treatments) recorded in the literature were collated and summarized, and a database was established for statistical analysis. Results 257 articles that met the inclusion criteria were selected. Among them, the most common animal model was SKH-1 hairless mice, followed by SD rats and KM mice; the gender of animals was mainly female, medium-wave ultraviolet B (UVB) was often used as the radiation source, the distance between the radiation source and the modelling site was mostly 30 cm, and the modelling period was usually 40-60 days. The cumulative dose of long-wave ultraviolet A (UVA) was between 100-150 J/cm², and the cumulative dose of UVB was between 5-10 J/cm². The tests used after model establishment were skin histopathological examination, skin tissue homogenization, fibre staining, immunoblotting, etc. Subjects included Chinese herbal medicines, Chinese herbal extracts, Chinese patent medicines, Chinese herbal compound medicines, chemical drugs, biological agents and other treatments, while the animal model of skin photoaging was also used for clinical efficacy studies of external Chinese medicine, physiotherapy and positive control drugs. Conclusion In skin photoaging animal experiments, female SKH-1 hairless mice are often used, and UVB is used as the radiation source. The modeling period is usually 40-60 days, and the minimum erythema dose (MED) is incremented week by week. The cumulative UVB irradiation dose ranges from 0 to 10 J/cm², which has the advantages of high success rate, good reproducibility and high similarity with clinical disease.

Key words: Skin photoaging, Animal model, Modeling element, Subject, SKH-1 hairless mice, Ultraviolet B (UVB)

中图分类号:

Q95-33

邓亚胜,林江,甘池伶,等. 皮肤光老化动物模型制备要素和受试物数据的文献分析[J]. 实验动物与比较医学, 2023, 43(4): 406-414. DOI: 10.12300/j.issn.1674-5817.2023.026.

Yasheng DENG,Jiang LIN,Chiling GAN,et al. Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 406-414. DOI: 10.12300/j.issn.1674-5817.2023.026.

图/表 3

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动物种类 Animal species	品种品系 Breed or strain	频次（所占百分比） Frequency (percentage/%)
小鼠Mouse	SKH-1无毛小鼠	60 (23.34)
	KM小鼠	52 (20.23)
	BALB/c小鼠	23 (8.95)
	HR-1无毛小鼠	22 (8.56)
	ICR小鼠	20 (7.78)
	C57BL/6小鼠	3 (1.17)
	Swiss小鼠	3 (1.17)
	HRM-2无毛小鼠	2 (0.78)
	未知种类无毛小鼠	2 (0.78)
大鼠Rat	SD大鼠	53 (20.62)
	Wistar大鼠	8 (3.11)
	F344大鼠	2 (0.78)
	HWY/Slc无毛大鼠	1 (0.39)
豚鼠Guinea pig	未知种类	4 (1.56)
猪Pig	广西巴马小型猪	2 (0.78)

动物种类 Animal species	品种品系 Breed or strain	频次（所占百分比） Frequency (percentage/%)
小鼠Mouse	SKH-1无毛小鼠	60 (23.34)
	KM小鼠	52 (20.23)
	BALB/c小鼠	23 (8.95)
	HR-1无毛小鼠	22 (8.56)
	ICR小鼠	20 (7.78)
	C57BL/6小鼠	3 (1.17)
	Swiss小鼠	3 (1.17)
	HRM-2无毛小鼠	2 (0.78)
	未知种类无毛小鼠	2 (0.78)
大鼠Rat	SD大鼠	53 (20.62)
	Wistar大鼠	8 (3.11)
	F344大鼠	2 (0.78)
	HWY/Slc无毛大鼠	1 (0.39)
豚鼠Guinea pig	未知种类	4 (1.56)
猪Pig	广西巴马小型猪	2 (0.78)

辐射光源与造模部位距离/cm Distance between the radiation source and the moulding site/cm	频次（所占百分比/%） Frequency (percentage/%)	累计照射剂量/（J·cm²） Cumulative exposure dose/ （J·cm²）	频次（所占百分比/%） Frequency (percentage/%）
2	1 (0.92)	UVA≤50	6 (9.09)
6.5	1 (0.92)	50＜UVA≤100	13 (19.70)
10	4 (3.67)	100＜UVA≤150	20 (30.30)
15	6 (5.50)	150＜UVA≤200	13 (19.70)
18	2 (1.84)	200＜UVA≤250	0 (0.00)
20	11 (10.09)	250＜UVA≤300	5 (7.58)
22	1 (0.92)	UVA＞300	9 (13.63)
23	2 (1.83)	UVB≤5	12 (15.58)
25	3 (2.75)	5＜UVB≤10	24 (31.17)
30	36 (33.03)	10＜UVB≤15	8 (10.39)
35	10 (9.17)	15＜UVB≤20	2 (2.60)
40	21 (19.27)	20＜UVB≤25	10 (12.99)
42	2 (1.84)	25＜UVB≤30	1 (1.30)
50	6 (5.50)	UVB＞30	20 (25.97)
100	3 (2.75)

辐射光源与造模部位距离/cm Distance between the radiation source and the moulding site/cm	频次（所占百分比/%） Frequency (percentage/%)	累计照射剂量/（J·cm²） Cumulative exposure dose/ （J·cm²）	频次（所占百分比/%） Frequency (percentage/%）
2	1 (0.92)	UVA≤50	6 (9.09)
6.5	1 (0.92)	50＜UVA≤100	13 (19.70)
10	4 (3.67)	100＜UVA≤150	20 (30.30)
15	6 (5.50)	150＜UVA≤200	13 (19.70)
18	2 (1.84)	200＜UVA≤250	0 (0.00)
20	11 (10.09)	250＜UVA≤300	5 (7.58)
22	1 (0.92)	UVA＞300	9 (13.63)
23	2 (1.83)	UVB≤5	12 (15.58)
25	3 (2.75)	5＜UVB≤10	24 (31.17)
30	36 (33.03)	10＜UVB≤15	8 (10.39)
35	10 (9.17)	15＜UVB≤20	2 (2.60)
40	21 (19.27)	20＜UVB≤25	10 (12.99)
42	2 (1.84)	25＜UVB≤30	1 (1.30)
50	6 (5.50)	UVB＞30	20 (25.97)
100	3 (2.75)

分类 Classification	检测指标 Testing indicators	频次（所占百分比/%） Frequency（percentage/%）
皮肤外观特征 Skin appearance features	皮肤外观观察	80 (9.06)
	皮肤（表皮、真皮）厚度	46 (5.21)
	皱纹测定	31 (3.51)
	皮肤弹性	9 (1.02)
皮肤含水量变化 Skin moisture content change	经皮水分损失	41 (4.64)
	皮肤（角质层）水合作用测定	14 (1.58)
	皮肤含水量测定	5 (0.57)
皮肤病理检查 Dermatologic examination	皮肤组织病理检查	173 (19.59)
	纤维染色	99 (11.21)
	免疫组化	64 (7.26)
	免疫荧光	9 (1.02)
皮肤组织匀浆检测 Skin tissue homogenisation	丙二醛、羟脯氨酸、过氧化氢酶活性、透明质酸、超氧化物歧化酶活性、谷胱甘肽过氧化酶活性、皮肤组织总抗氧化能力	108 (12.23)
免疫检测 Immunity testing	免疫印迹	81 (9.17)
免疫检测 Immunity testing	酶联免疫吸附试验	26 (2.94)
PCR分析 PCR analysis	反转录PCR	30 (3.40)
PCR分析 PCR analysis	实时荧光PCR	19 (2.15)
血液检查 Blood examination	血清	19 (2.15)
血液检查 Blood examination	全血或血浆	6 (0.68)
其他 Others	β-半乳糖苷酶染色	8 (0.91)
	体重变化	9 (1.02)
	活性氧定量分析/髓过氧化物酶活性测定	6 (0.68)

皮肤光老化动物模型制备要素和受试物数据的文献分析

Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects

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