实验动物应激后氧化应激状态监测方法的进展

doi:10.3969/j.issn.1674-5817.2012.06.021

摘要/Abstract

摘要： 应激可以引起实验动物出现氧化应激。应激后机体多个环节能量代谢的改变是其引起氧化应激的机制之一。糖皮质激素参与了应激后氧化应激的发生。检测机体活性氧(ROS)水平,抗氧化能力及氧化应激损害标志物含量,可用来评估氧化应激发生及其危害。本文将就近年来实验动物应激如何引起氧化应激、其发生机制及监测方法等领域的研究进展作一综述。

关键词: 应激, 氧化应激, 实验动物

Abstract: Stress could induce oxidative stress in laboratory animals. Changes in energy metabolism in several aspects of the organism are one of the mechanisms through which the stress induces oxidative stress. Glucocorticoids take part in occurrence of oxidative stress after stress. Reactive oxygen species(ROS) levels, antioxidant capacity and contents of biomarkers of oxidative stress injury can be used to evaluate occurrence and damage of the oxidative stress. It was reviewed in this article that progress in the fields of how strees induce oxidative stress in laboratory animals, the mechanisms and the monitoring methods in recent years.

Key words: Stress, Oxidative stress, Laboratory animals

中图分类号:

Q95-33

曾宪成, 马璟, 常艳, 刘全海. 实验动物应激后氧化应激状态监测方法的进展[J]. 实验动物与比较医学, 2012, 32(6): 549-552.

ZENG Xian-cheng, MA Jing, CHANG Yan, LIU Quan-hai. Progress on Monitoring Methods of Oxidative Stress after Stress in Laboratory Animals[J]. Laboratory Animal and Comparative Medicine, 2012, 32(6): 549-552.

参考文献

[1] Chirase NK, Greene LW, Purdy CW, et al.Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle[J]. Am J Vet Res, 2004, 65(6):860-864.
[2] Zafir A, Banu N.Modulation of in vivo oxidative status by exogenous corticosterone and restraint stress in rats[J]. Stress, 2009, 12(2):167-177.
[3] Satyanarayana PS, Chopra K.Oxidative stress-mediated renal dysfunction by cyclosporine A in rats: attenuation by trimetazidine[J]. Ren Fail, 2002, 24:259-274.
[4] KottováM, PourováJ, VoprsalováM. Oxidative stress and its role in respiratory diseases[J]. Ceska Slov Farm, 2007, 56(5):215-219.
[5] Popovic M, Janicijevic-Hudomal S, Kaurinovic B, et al.Effects of various drugs on alcohol-induced oxidative stress in the liver[J]. Molecules, 2008, 13(9):2249-2259.
[6] Ricart-Jane D, Rodriguez-Sureda V, Benavides A, et al.Immobilization stress alters intermediate metabolism and circulating lipoproteins in the rat[J]. Metabolism, 2002, 51(7):925-931.
[7] Kohen R, Nyska A.Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification[J]. Toxicol Pathol, 2002, 30(6):620-650.
[8] 曾宪成, 潘学营, 姚妷舒, 等. 运输应激对食蟹猴血清生化指标、丙二醛及总抗氧化能力的影响[J]. 四川动物, 2010, 29(6):990-994.
[9] Sordillo LM, Aitken SL.Impact of oxidative stress on the health and immune function of dairy cattle[J]. Vet Immunol Immunopathol, 2009, 128(1-3):104-109.
[10] Larbi A, Kempf J, Pawelec G.Oxidative stress modulation and T cell activation[J]. Exp Gerontol, 2007, 42(9):852-858.
[11] Sahin E, Gumuslu S.Immobilization stress in rat tissues: alterations in protein oxidation, lipid peroxidation and antioxidant defense system[J]. Comp Biochem Physiol C Toxicol Pharmacol, 2007, 144(4):342-347.
[12] Ohtsuka A, Kojima H, Ohtani T, et al.Vitamin E reduces glucocorticoid-induced oxidative stress in rat skeletal muscle[J]. J Nutr Sci Vitaminol (Tokyo), 1998, 44(6):779-786.
[13] Batandier C, Fontaine E, Keriel C, et al.Determination of mitochondrial reactive oxygen species: methodological aspects[J]. J Cell Mol Med, 2002, 6(2):175-187.
[14] 章舒祺, 魏永锋. 检测活性氧的荧光探针新进展[J]. 光谱实验室, 2009, 26(4):794-802.
[15] Sun Y, Yin XF, Ling YY, et al.Determination of reactive oxygen species in single human erythrocytes using microfluidic chip electrophoresis[J]. Anal Bioanal Chem, 2005, 382(7):1472-1476.
[16] Guthrie HD, Welch GR.Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry[J]. J Anim Sci, 2006, 84(8):2089-2100.
[17] Zhou G, Li J, Chen Y, et al.Determination of reactive oxygen species generated in laccase catalyzed oxidation of wood fibers from Chinese fir (Cunninghamia lanceolata) by electron spin resonance spectrometry[J]. Bioresour Technol, 2009, 100(1):505-508.
[18] Harwood DT, Kettle AJ, Brennan S, et al.Simultaneous determination of reduced glutathione, glutathione disulphide and glutathione sulphonamide in cells and physiological fluids by isotope dilution liquid chromatography-tandem mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2009, 877(28):3393-3399.
[19] Kalimuthu P, John SA.Simultaneous determination of ascorbic acid, dopamine, uric acid and xanthine using a nanostructured polymer film modified electrode[J]. Talanta, 80(5):1686-1691.
[20] Niki E.Lipid peroxidation products as oxidative stress biomarkers[J]. BioFactors, 2008, 34:171-180.
[21] Klaunig JE, Kamendulis LM, Hocevar BA.Oxidative stress and oxidative damage in carcinogenesis[J]. Toxicol Pathol, 2010, 38(1):96-109.
[22] Cheng GW, Wu HL, Huang YL.Simultaneous determination of malondialdehyde and ofloxacin in plasma using an isocratic high-performance liquid chromatography/fluorescence detection system[J]. Anal Chim Acta, 2008, 616(2):230-234.
[23] Del Rio D, Stewart AJ, Pellegrini N.A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress[J]. Nutr Metab Cardiovasc Dis, 2005, 15(4): 316-328.
[24] Ogawa F, Shimizu K, Muroi E, et al.Serum levels of 8-isoprostane, a marker of oxidative stress, are elevated in patients with systemic sclerosis[J]. Rheumatology (Oxford), 2006, 45(7):815-818.
[25] Carraro S, Cogo PE, Isak I, et al.EIA and GC-MS analysis of 8-isoprostane in EBC of children with problematic asthma[J]. Eur Respir J, 2010,35(6):1364-1369.
[26] Montuschi P.LC/MS/MS analysis of leukotriene B4 and other eicosanoids in exhaled breath condensate for assessing lung inflammation[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2009, 877(13):1272-1280.
[27] 贾秀英, 施蔡雷. 镉致黑斑蛙肝脏中ROS生成及其蛋白质氧化损伤作用[J]. 环境科学学报, 2010, 30(1):186-191.
[28] Jamel MJ, Pereira Lde P, Mello NB, et al.Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats[J]. Acta Cir Bras, 25(1):59-62.
[29] Hofer T, Moller L.Optimization of the workup procedure for the analysis of 8-oxo-7,8-dihydro-2’-deoxyguanosine with electrochemical detection[J]. Chem Res Toxicol, 2002, 15(3):426-432.
[30] ESCODD.Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: an approach to consensus[J]. Carcinogenesis, 2002, 23(12):2129-2133.
[31] Cooke MS, Singh R, Hall GK, et al.Evaluation of enzyme-linked immunosorbent assay and liquid chromatography-tandem mass spectrometry methodology for the analysis of 8-oxo-7,8-dihydro-2’-deoxyguanosine in saliva and urine[J]. Free Radic Biol Med, 2006, 41(12): 1829-1836.
[32] Lee KF, Chung WY, Benzie IF.Urine 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxodG), a specific marker of oxidative stress, using direct, isocratic LC-MS/MS: Method evaluation and application in study of biological variation in healthy adults[J]. Clin Chim Acta, 2010, 411(5-6):416-422.