《动物研究：体内实验报告》即ARRIVE 2.0指南的解释与阐述（三）

doi:10.12300/j.issn.1674-5817.2023.039

实验动物与比较医学 ›› 2023, Vol. 43 ›› Issue (4): 446-456.DOI: 10.12300/j.issn.1674-5817.2023.039

所属专题：动物实验设计及统计学方法

• 比较医学研究与报告规范 • 上一篇下一篇

《动物研究：体内实验报告》即ARRIVE 2.0指南的解释与阐述（三）

刘晓宇¹, 卢选成², 师晓萌², 张雨舟², 吕超², 陈国元³, 卢晓⁴, 白玉⁵, 高静⁶, 李垚⁷, 刘永刚⁸, 陶雨风⁹()(), 庞万勇¹⁰()()

^1.中国疾病预防控制中心职业卫生与中毒控制所, 北京 100050
^2.中国疾病预防控制中心实验动物中心, 北京 102206
^3.中国科学院分子细胞科学卓越创新中心动物实验技术平台, 上海 200031
^4.迪哲医药股份有限公司, 上海 201203
^5.北京诺和诺德医药科技有限公司, 北京 102206
^6.上海交通大学医学院临床研究中心, 上海 200025
^7.上海交通大学医学院实验动物科学部, 上海 200025
^8.中国农业科学院哈尔滨兽医研究所, 哈尔滨 150001
^9.中国合格评定国家认可中心, 北京 100062
^10.赛诺菲公司全球研发中心转化体内模型研究平台, 北京 100022

收稿日期:2023-03-29 修回日期:2023-07-21 出版日期:2023-08-25 发布日期:2023-09-14
通讯作者: 陶雨风（1970—），女，博士，高级工程师，研究方向：分子免疫学。E-mail： taoyf@cnas.org.cn。ORCID： 0000-0003-1925-1048；
庞万勇（1974—），男，博士，研究方向：实验动物医学。E-mail： pang1yong@outlook.com。ORCID：0000-0002-0724-2016；
作者简介:刘晓宇（1979—），女，病原生物学博士，副研究员，研究方向：实验动物医学。E-mail： liuxy@niohp.chinacdc.cn；
卢选成（1977—），男，兽医博士，研究员，研究方向：实验动物与生物安全。E-mail： luxc@chinacdc.cn
基金资助:
国家重点研发计划“实验动物共性关键质量评价技术标准研究”(2022YFF0711000);中国科学技术期刊编辑学会2023年翰笔计划医学中青年编辑科研项目“动物实验医学研究及报告规范化的国际对标（指南汉化推广）”(HBJH-2023-A16)

Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ)

Xiaoyu LIU¹, Xuancheng LU², Xiaomeng SHI², Yuzhou ZHANG², Chao LÜ², Guoyuan CHEN³, Xiao LU⁴, Yu BAI⁵, Jing GAO⁶, Yao LI⁷, Yonggang LIU⁸, Yufeng TAO⁹()(), Wanyong PANG¹⁰()()

^1.National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China
^2.Laboratory Animal Centre, Chinese Center for Disease Control and Prevention, Beijing 102206, China
^3.Animal Core Facility, Center for Excellence in Molecular Cell Science, Chinese Academy of Science, Shanghai 200031, China
^4.Dizal Pharmaceuticals Co. , Ltd. , Shanghai 201203, China
^5.Novo Nordisk Research Centre China, Beijing 102206, China
^6.Clinical Research Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
^7.Department of Laboratory Animal Science, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China
^8.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
^9.China National Accreditation Service for Conformity Assessment, Beijing 100062, China
^10.Translational in Vivo Model Research Platform, Sanofi G&D, Beijing 100022, China

Received:2023-03-29 Revised:2023-07-21 Published:2023-08-25 Online:2023-09-14
Contact: TAO Yufeng (ORCID: 0000-0003-1925-1048), E-mail: taoyf@cnas.org.cn;
PANG Wanyong (ORCID: 0000-0002-0724-2016), E-mail: pang1yong@outlook.com;

摘要/Abstract

摘要：

提高生物医学研究结果的可重复性是一项重大挑战，研究人员透明且准确地报告其研究过程有利于读者对该研究结果的可靠性进行评估，进而重复该实验或在该成果的基础上进一步探索。ARRIVE 2.0指南是英国国家3Rs中心（NC3Rs）于2019年组织发布的一份适用于任何与活体动物研究报告相关的指导性清单，用以提高动物体内实验设计、实验实施和实验报告的规范性，以及动物实验结果的可靠性、可重复性和临床转化率。ARRIVE 2.0指南的使用不仅可以丰富动物实验研究报告的细节，确保动物实验结果信息被充分评估和利用，还可以使读者准确且清晰地了解作者所表述的内容，促进基础研究评审过程的透明化和完整性。目前，ARRIVE 2.0指南已经被国际生物医学期刊广泛采纳。本文是在国际期刊遵循ARRIVE 2.0指南的最佳实践基础上，对2020年发表于PLoS Biology期刊上的ARRIVE 2.0指南完整解读版（原文请见https：//arriveguidelines.org）进行中文编译（第三部分包括“关键10条”里的第8～10条：“实验动物”、“实验步骤”和“结果”部分），以期促进国内研究人员充分理解并使用ARRIVE 2.0指南，提高实验动物研究及报告的规范性，助推我国实验动物科技与比较医学研究的高质量发展。

关键词: 动物实验, ARRIVE 2.0指南, ARRIVE关键10条, 实验动物, 实验步骤, 实验结果

Abstract:

Improving the reproducibility of biomedical research results is a major challenge.Researchers reporting their research process transparently and accurately can help readers evaluate the reliability of the research results and further explore the experiment by repeating it or building upon its findings. The ARRIVE 2.0 guidelines, released in 2019 by the UK National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs), provide a checklist applicable to any in vivo animal research report. These guidelines aim to improve the standardization of experimental design, implementation, and reporting, as well as the reliability, repeatability, and clinical translatability of animal experimental results. The use of ARRIVE 2.0 guidelines not only enriches the details of animal experimental research reports, ensuring that information on animal experimental results is fully evaluated and utilized, but also enables readers to understand the content expressed by the author accurately and clearly, promoting the transparency and integrity of the fundamental research review process. At present, the ARRIVE 2.0 guidelines have been widely adopted by international biomedical journals. This article is a Chinese translation based on the best practices of international journals following the ARRIVE 2.0 guidelines in international journals, specifically for the complete interpretation of the ARRIVE 2.0 guidelines published in the PLoS Biology journal in 2020 (original text can be found at https://arriveguidelines.org). The third part of the article includes the items 8-10 of ARRIVE 2.0 Essential 10, which covers "experimental animals" "experimental procedures" and "results". Its aim is to promote the full understanding and use of the ARRIVE 2.0 guidelines by domestic researchers, enhance the standardization of experimental animal research and reporting, and promote the high-quality development of experimental animal technology and comparative medicine research in China.

Key words: Animal experiment, ARRIVE 2.0 guidelines, ARRIVE essential 10, Experimental animals, Experimental procedures, Experimental results

中图分类号:

R-332

刘晓宇, 卢选成, 师晓萌, 张雨舟, 吕超, 陈国元, 卢晓, 白玉, 高静, 李垚, 刘永刚, 陶雨风, 庞万勇. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释与阐述（三）[J]. 实验动物与比较医学, 2023, 43(4): 446-456.

Xiaoyu LIU, Xuancheng LU, Xiaomeng SHI, Yuzhou ZHANG, Chao LÜ, Guoyuan CHEN, Xiao LU, Yu BAI, Jing GAO, Yao LI, Yonggang LIU, Yufeng TAO, Wanyong PANG. Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ)[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 446-456.

图/表 5

参考文献 154

103	王剑, 卢今, 马政文, 等. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（一）[J]. 实验动物与比较医学, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
	WANG J, LU J, MA Z W, et al. Explanation and elaboration for the ARRIVE Guidelines 2.0—reporting animal research and in vivo experiments (Ⅰ)[J]. Lab Anim Comp Med, 2023, 43(2): 213-224. DOI: 10.12300/j.issn.1674-5817.2023.043 .
104	陈国元, 卢晓, 白玉, 等. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（二）[J]. 实验动物与比较医学, 2023, 43(3): 323-331. DOI: 10.12300/j.issn.1674-5817.2023.042 .
	CHEN G Y, LU X, BAI Y, et al. Explanation and elaboration for the ARRIVE Guidelines 2.0—reporting animal research and in vivo experiments (Ⅱ)[J]. Lab Anim Comp Med, 2023, 43(3): 323-331. DOI: 10.12300/j.issn.1674-5817.2023.042 .
105	CLAYTON J A, COLLINS F S. Policy: NIH to balance sex in cell and animal studies[J]. Nature, 2014, 509(7500):282-283. DOI:
10	1038/509282a.
106	SHAPIRA S, SAPIR M, WENGIER A, et al. Aging has a complex effect on a rat model of ischemic stroke[J]. Brain Res, 2002, 925(2):148-158. DOI: 10.1016/s0006-8993(01)03270-x .
107	VITAL M, HARKEMA J R, RIZZO M, et al. Alterations of the murine gut microbiome with age and allergic airway disease [J]. J Immunol Res, 2015, 2015:892568. DOI: 10.1155/2015/ 892568 .
108	BOUWKNECHT J A, PAYLOR R. Behavioral and physiological mouse assays for anxiety: a survey in nine mouse strains[J]. Behav Brain Res, 2002, 136(2):489-501. DOI: 10.1016/S0166- 4328(02)00200-0 .
109	SIMON M M, GREENAWAY S, WHITE J K, et al. A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/ 6N mouse strains[J]. Genome Biol, 2013, 14(7): R82. DOI: 10.1186/gb-2013-14-7-r82 .
110	JACKSON S J, ANDREWS N, BALL D, et al. Does age matter? The impact of rodent age on study outcomes[J]. Lab Anim, 2017, 51(2):160-169. DOI: 10.1177/0023677216653984 .
111	KHOKHA M K, CHUNG C, BUSTAMANTE E L, et al. Techniques and probes for the study of Xenopus tropicalis development [J]. Dev Dyn, 2002, 225(4):499-510. DOI: 10.1002/dvdy.10184 .
112	ÕKVA K, NEVALAINEN T, POKK P. The effect of cage shelf on the behaviour of male C57BL/6 and BALB/c mice in the elevated plus maze test[J]. Lab Anim, 2013, 47(3):220-222. DOI: 10.1177/0023677213489280 .
113	AKKERS R C, VAN HEERINGEN S J, JACOBI U G, et al. A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos[J]. Dev Cell, 2009, 17(3): 425-434. DOI: 10.1016/j.devcel.2009.08.005 .
114	FELASA WORKING GROUP ON REVISION OF GUIDELINES FOR HEALTH MONITORING OF RODENTS AND RABBITS, MÄHLER CONVENOR M, BERARD M, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, Guinea pig and rabbit colonies in breeding and experimental units[J]. Lab Anim, 2014, 48(3):178-192. DOI: 10.1177/0023677213516312 .
115	BAKER D G. Natural pathogens of laboratory mice, rats, and rabbits and their effects on research[J]. Clin Microbiol Rev, 1998, 11(2):231-266. DOI: 10.1128/CMR.11.2.231 .
116	VELAZQUEZ E M, NGUYEN H, HEASLEY K T, et al. Endogenous Enterobacteriaceae underlie variation in susceptibility to Salmonella infection[J]. Nat Microbiol, 2019, 4(6):1057-1064. DOI: 10.1038/s41564-019-0407-8 .
117	HOLMDAHL R, MALISSEN B. The need for littermate controls[J]. Eur J Immunol, 2012, 42(1):45-47. DOI: 10.1002/eji. 201142048 .
118	MALLAPATY S. In the Name of reproducibility[J]. Lab Animal, 2018, 47(7):178-181. DOI: 10.1038/s41684-018-0095-7 .
119	SUNDBERG J P, SCHOFIELD P N. Commentary: mouse genetic nomenclature[J]. Vet Pathol, 2010, 47(6):1100-1104. DOI: 10.1177/0300985810374837 .
120	MONTOLIU L, WHITELAW C B. Using standard nomenclature to adequately name transgenes, knockout gene alleles and any mutation associated to a genetically modified mouse strain[J]. Transgenic Res, 2011, 20(2):435-440. DOI: 10.1007/s11248-010-9428-z .
121	HUANG W, FENG Y L, LIANG J L, et al. Loss of microRNA-128 promotes cardiomyocyte proliferation and heart regeneration [J]. Nat Commun, 2018, 9(1):700. DOI: 10.1038/s41467-018- 03019-z .
122	RANSON A, CHEETHAM C E J, FOX K, et al. Homeostatic plasticity mechanisms are required for juvenile, but not adult, ocular dominance plasticity[J]. Proc Natl Acad Sci USA, 2012, 109(4):1311-1316. DOI: 10.1073/pnas.1112204109 .
123	CLARKSON J M, DWYER D M, FLECKNELL P A, et al. Handling method alters the hedonic value of reward in laboratory mice [J]. Sci Rep, 2018, 8(1):2448. DOI: 10.1038/s41598-018-20716-3 .
124	HURST J L, WEST R S. Taming anxiety in laboratory mice[J]. Nat Methods, 2010, 7(10):825-826. DOI: 10.1038/nmeth.1500 .
125	HEWITT J A, BROWN L L, MURPHY S J, et al. Accelerating biomedical discoveries through rigor and transparency[J]. ILAR J, 2017, 58(1):115-128. DOI: 10.1093/ilar/ilx011 .
126	ALMEIDA J L, COLE K D, PLANT A L. Standards for cell line authentication and beyond[J]. PLoS Biol, 2016, 14(6): e1002476. DOI: 10.1371/journal.pbio.1002476 .
127	LEARY S, UNDERWOOD W, ANTHONY R, et al. J K. AVMA guidelines for the euthanasia of animals[J]. Anim Welf, 2013, 22(3):412. DOI: 10.1017/ s0962728600005492 .
128	BANDROWSKI A E, MARTONE M E. RRIDs: a simple step toward improving reproducibility through rigor and transparency of experimental methods[J]. Neuron, 2016, 90(3):434-436. DOI: 10.1016/j.neuron.2016.04.030 .
129	BANDROWSKI A, BRUSH M, GRETHE J S, et al. The resource identification initiative: a cultural shift in publishing[J]. Neuroinform, 2016, 14(2):169-182. DOI: 10.1007/s12021-015- 9284-3 .
130	TEYTELMAN L, STOLIARTCHOUK A. Protocols.io: Reducing the knowledge that perishes because we do not publish it[J]. Inf Serv Use, 2015, 35(1-2):109-115. DOI: 10.3233/isu-150769 .
131	REYNOLDS P S, FISHER B J, MCCARTER J, et al. Interventional vitamin C: a strategy for attenuation of coagulopathy and inflammation in a swine multiple injuries model[J]. J Trauma Acute Care Surg, 2018, 85(1S ): S57-S67. DOI: 10.1097/ TA.0000000000001844 .
132	BAUTERS D, BEDOSSA P, LIJNEN H R, et al. Functional role of ADAMTS5 in adiposity and metabolic health[J]. PLoS One, 2018,13(1): e0190595. DOI:10.1371/journal.pone.0190595 .
133	LIAN X Y, WU X Y, LI Z X, et al. The combination of metformin and 2-deoxyglucose significantly inhibits cyst formation in miniature pigs with polycystic kidney disease[J]. Br J Pharmacol, 2019, 176(5):711-724. DOI: 10.1111/bph.14558 .
134	BARTLANG M S, NEUMANN I D, SLATTERY D A, et al. Time matters: pathological effects of repeated psychosocial stress during the active, but not inactive, phase of male mice[J]. J Endocrinol, 2012, 215(3):425-437. DOI: 10.1530/JOE-12-0267 .
135	PAUL A K, GUEVEN N, DIETIS N. Morphine dosing strategy plays a key role in the generation and duration of the produced antinociceptive tolerance[J]. Neuropharmacology, 2017, 121:158-166. DOI: 10.1016/j.neuropharm.2017.04.034 .
136	HAWKINS P, MORTON D B, BURMAN O, et al. A guide to defining and implementing protocols for the welfare assessment of laboratory animals: eleventh report of the BVAAWF/FRAME/RSPCA/UFAW Joint Working Group on Refinement[J]. Lab Anim, 2011, 45(1):1-13. DOI: 10.1258/ la.2010.010031 .
137	HAGEMO J S, JØRGENSEN J J, OSTROWSKI S R, et al. Changes in fibrinogen availability and utilization in an animal model of traumatic coagulopathy[J]. Scand J Trauma Resusc Emerg Med, 2013, 21:56. DOI: 10.1186/1757-7241-21-56 .
138	EMERY M, NANCHEN N, PREITNER F, et al. Biological characterization of gene response to insulin-induced hypoglycemia in mouse retina[J]. PLoS One, 2016, 11(2): e0150266. DOI: 10.1371/journal.pone.0150266 .
139	HOLMES A M, EMMANS C J, COLEMAN R, et al. Effects of transportation, transport medium and re-housing on Xenopus laevis (Daudin)[J]. Gen Comp Endocrinol, 2018, 266: 21-28. DOI: 10.1016/j.ygcen.2018.03.015 .
140	CONOUR L A, MURRAY K A, BROWN M J. Preparation of animals for research: issues to consider for rodents and rabbits[J]. ILAR J, 2006, 47(4):283-293. DOI: 10.1093/ilar.47.4.283 .
141	OBERNIER J A, BALDWIN R L. Establishing an appropriate period of acclimatization following transportation of laboratory animals[J]. ILAR J, 2006, 47(4):364-369. DOI: 10.1093/ilar.47.4.364 .
142	KRAHN D D, GOSNELL B A, MAJCHRZAK M J. The anorectic effects of CRH and restraint stress decrease with repeated exposures[J]. Biol Psychiatry, 1990, 27(10):1094-1102. DOI: 10.1016/0006-3223(90)90046-5 .
143	PITMAN D L, OTTENWELLER J E, NATELSON B H. Plasma corticosterone levels during repeated presentation of two intensities of restraint stress: chronic stress and habituation [J]. Physiol Behav, 1988, 43(1):47-55. DOI: 10.1016/0031-9384 (88)90097-2 .
144	BROCK A J, GOODY S G, MEAD A N, et al. Assessing the value of the zebrafish conditioned place preference model for predicting human abuse potential[J]. J Pharmacol Exp Ther, 2017, 363(1):66-79. DOI: 10.1124/jpet.117.242628 .
145	TURNER P V, BRABB T, PEKOW C, et al. Administration of substances to laboratory animals: routes of administration and factors to consider[J]. J Am Assoc Lab Anim Sci, 2011, 50 (5):600-613.
146	FUEGER B J, CZERNIN J, HILDEBRANDT I, et al. Impact of animal handling on the results of 18F-FDG PET studies in mice[J]. J Nucl Med, 2006, 47(6):999-1006.
147	ASLAN Y, TADJUIDJE E, ZORN A M, et al. High efficiency non- mosaic CRISPR mediated knock-in and mutations in F0 Xenopus[J]. Development, 2017: 144(15): 2852-2858. DOI: 10.1242/dev.152967 .
148	MICHEL M C, MURPHY T J, MOTULSKY H J. New author guidelines for displaying data and reporting data analysis and statistical methods in experimental biology[J]. J Pharmacol Exp Ther, 2020, 372(1):136-147. DOI: 10.1124/jpet.119.264143 .
149	SCHERZ M D, HUTTER C R, RAKOTOARISON A, et al. Morphological and ecological convergence at the lower size limit for vertebrates highlighted by five new miniaturised microhylid frog species from three different Madagascan Genera[J]. PLoS One, 2019, 14(3): e0213314. DOI: 10.1371/ journal.pone.0213314 .
150	NOVOTOVÁ M, ZAHRADNÍKOVÁ A Jr, NICHTOVÁ Z, et al. Structural variability of dyads relates to calcium release in rat ventricular myocytes[J]. Sci Rep, 2020, 10(1):8076. DOI: 10.1038/s41598-020-64840-5 .
151	WASSERSTEIN R L, SCHIRM A L, LAZAR N A. Moving to a world beyond “p<0.05”[J]. Am Stat, 2019, 73(sup1):1-19. DOI: 10.1080/00031305.2019.1583913 .
152	ALTMAN DSC D G. Why we need confidence intervals[J]. World J Surg, 2005, 29(5):554-556. DOI: 10.1007/s00268-005- 7911-0 .
153	MOHER D, HOPEWELL S, SCHULZ K F, et al. CONSORT 2010 explanation and elaboration: updated guidelines for reporting parallel group randomised trials[J]. Int J Surg, 2012, 10(1):28-55. DOI: 10.1016/j.ijsu.2011.10.001 .
154	NAKAGAWA S, CUTHILL I C. Effect size, confidence interval and statistical significance: a practical guide for biologists[J]. Biol Rev Camb Philos Soc, 2007, 82(4):591-605. DOI: 10.1111/ j.1469-185X.2007.00027.x .

编号 No.	实验流程 Procedures	实验资源 Resources
1	药理研究（包括干预和对照）	细胞系
	● 药物配方	● 鉴别
	● 剂量	● 来源
	● 体积	● 验证和认证
	● 浓度	● RRID^[128-129]
	● 给药部位和途径
	● 给药频率
	● 溶媒或载体溶液的配方和体积
	● 任何可以表明所使用的药剂到达靶组织的证据
2	外科手术（包括假手术）	试剂（如抗体、化学品）
	● 手术过程描述	● 制造商
	● 使用的麻醉剂（包括上文药理研究部分所列剂量及信息）	● 供应商
	● 术前和术后镇痛方案	● 目录号
	● 术前程序（如禁食）	● 批号（如适用）
	● 无菌术	● 药物纯度（如适用）
	● 监测（如评估手术麻醉状况）	● RRID
	● 流程是否是终末性质的
	● 术后程序
	● 手术持续时间和麻醉持续时间
	● 测量的身体指标
3	病原体感染（包括干预和对照）	设备和软件
	● 传染性病原体	● 制造商
	● 感染最高剂量	● 供应商
	● 溶媒或载体溶液的配方和体积	● 型号/版本号
	● 感染部位和途径	● 校准程序（如适用）
	● 感染时间或频率	● RRID
4	安乐死
	● 安乐死方法，包括该方法遵循的人道标准，如AVMA^[127]
	● 药剂（如使用）（包括上文药理研究部分所列剂量及信息）
	● 安乐死前或安乐死期间为减轻疼痛和痛苦而采取的任何措施
	● 安乐死的时间
	● 安乐死后收集的组织及其时间

编号 No.	实验流程 Procedures	实验资源 Resources
1	药理研究（包括干预和对照）	细胞系
	● 药物配方	● 鉴别
	● 剂量	● 来源
	● 体积	● 验证和认证
	● 浓度	● RRID^[128-129]
	● 给药部位和途径
	● 给药频率
	● 溶媒或载体溶液的配方和体积
	● 任何可以表明所使用的药剂到达靶组织的证据
2	外科手术（包括假手术）	试剂（如抗体、化学品）
	● 手术过程描述	● 制造商
	● 使用的麻醉剂（包括上文药理研究部分所列剂量及信息）	● 供应商
	● 术前和术后镇痛方案	● 目录号
	● 术前程序（如禁食）	● 批号（如适用）
	● 无菌术	● 药物纯度（如适用）
	● 监测（如评估手术麻醉状况）	● RRID
	● 流程是否是终末性质的
	● 术后程序
	● 手术持续时间和麻醉持续时间
	● 测量的身体指标
3	病原体感染（包括干预和对照）	设备和软件
	● 传染性病原体	● 制造商
	● 感染最高剂量	● 供应商
	● 溶媒或载体溶液的配方和体积	● 型号/版本号
	● 感染部位和途径	● 校准程序（如适用）
	● 感染时间或频率	● RRID
4	安乐死
	● 安乐死方法，包括该方法遵循的人道标准，如AVMA^[127]
	● 药剂（如使用）（包括上文药理研究部分所列剂量及信息）
	● 安乐死前或安乐死期间为减轻疼痛和痛苦而采取的任何措施
	● 安乐死的时间
	● 安乐死后收集的组织及其时间

[1]	马政文, 李夏莹, 刘晓宇, 李垚, 王剑, 卢今, 陈国元, 卢晓, 白玉, 卢选成, 刘永刚, 庞万勇, 陶雨风. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（五）[J]. 实验动物与比较医学, 2024, 44(1): 105-114.
[2]	黄跃, 董烨, 舒加乐. 实验动物设施运行维护和保养模式探讨[J]. 实验动物与比较医学, 2024, 44(1): 92-96.
[3]	唐倩倩, 张秀莉, 常在. 清华大学实验动物中心小鼠自动饮水系统漏水情况统计分析[J]. 实验动物与比较医学, 2024, 44(1): 85-91.
[4]	李夏莹, 田永路, 刘晓宇, 卢选成, 陈国元, 卢晓, 白玉, 高静, 李垚, 韦玉生, 庞万勇, 陶雨风. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（四）[J]. 实验动物与比较医学, 2023, 43(6): 659-668.
[5]	王硕, 吕昀徽, 王小康, 张朕豪, 崔永春. 体外膜肺氧合动物实验平台质量评价指标体系的构建与验证[J]. 实验动物与比较医学, 2023, 43(6): 604-611.
[6]	苗金环, 徐霞, 周璐, 成海燕, 何燕. 基于VOSviewer的中医护理技术动物实验可视化分析[J]. 实验动物与比较医学, 2023, 43(6): 626-635.
[7]	赵亚, 张彩勤, 孟寒, 秦靖, 白冰, 赵勇, 葛煦, 师长宏. 课程思政视域下实验动物学教学实践探索[J]. 实验动物与比较医学, 2023, 43(6): 641-646.
[8]	谢建芸. 东方田鼠作为一种实验动物新资源的研究进展报告[J]. 实验动物与比较医学, 2023, 43(5): 482-491.
[9]	赵勇. 实验动物管理工作的沿革与展望——以上海市近10年发展为例[J]. 实验动物与比较医学, 2023, 43(5): 492-503.
[10]	谢淑武, 沈如凌, 林金杏, 范春. 雄性不育药物研发相关实验动物模型建立和应用进展[J]. 实验动物与比较医学, 2023, 43(5): 504-511.
[11]	郭连香. 实验动物微生物、寄生虫学等级及监测标准的变化及与国外标准的比较[J]. 实验动物与比较医学, 2023, 43(4): 339-346.
[12]	黄缨, 韦思羽, 蔡莉, 强苏静, 李冬婷, 丁玉强. 供应商来源的实验大鼠和小鼠微生物监测结果分析：以复旦大学实验动物科学部为例[J]. 实验动物与比较医学, 2023, 43(4): 347-354.
[13]	陈国元, 卢晓, 白玉, 于灵芝, 乔颖, 王剑, 卢今, 刘晓宇, 卢选成, 高静, 李垚, 庞万勇. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（二）[J]. 实验动物与比较医学, 2023, 43(3): 323-331.
[14]	苟欢, 安新颖, 童俞嘉, 王琰, 杨爽. 日本实验动物发展现状分析[J]. 实验动物与比较医学, 2023, 43(2): 194-204.
[15]	王剑, 卢今, 马政文, 陈国元, 卢晓, 白玉, 刘晓宇, 卢选成, 高静, 李垚, 庞万勇. 《动物研究：体内实验报告》即ARRIVE 2.0指南的解释和阐述（一）[J]. 实验动物与比较医学, 2023, 43(2): 213-224.

《动物研究：体内实验报告》即ARRIVE 2.0指南的解释与阐述（三）

Explanation and Elaboration for the ARRIVE Guidelines 2.0—Reporting Animal Research and In Vivo Experiments (Ⅲ)

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 154

相关文章 15

编辑推荐

Metrics

本文评价