应用于行为学研究中八臂迷宫实验方法的改良

doi:10.3969/j.issn.1674-5817.2019.03.009

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (3): 226-230.DOI: 10.3969/j.issn.1674-5817.2019.03.009

应用于行为学研究中八臂迷宫实验方法的改良

刘波^1,2, 王杨³, 李志杰^1,2

1.中国医科大学附属盛京医院实验研究中心, 沈阳 110004;
2.辽宁省环境与代谢疾病动物模型研究与应用重点实验室, 沈阳 110004;
3.辽宁省肿瘤医院结直肠外科, 沈阳 110042

收稿日期:2019-02-27 出版日期:2019-06-25 发布日期:2021-01-29
作者简介:刘波(1986-),男,助理实验师。E-mail:18940257719@189.cn

An Modified Eight-arm Maze Experiment Method in Behavioral Research

LIU Bo^1,2, WANG Yang³, LI Zhi-jie^1,2

1. Medical Research Center, Shengjing Hospital of China Medical University, Shenyang 110004, China;
2. Liaoning Key Laboratory of Research and Application of Animal Modelsfor Environmental and Metabolic Diseases, Shenyang 110004, China;
3. Department of Colorectal Surgery, Liaoning Cancer Hospital and Institute, Shenyang 110042, China

Received:2019-02-27 Online:2019-06-25 Published:2021-01-29

摘要/Abstract

摘要： 目的探讨一种简易有效的新型八臂迷宫实验方法。方法选用20只雄性成年SD大鼠,分为传统方法组即对照组和改良电加热方法组即实验组,每组10只。于对照组食饵臂末端放置食饵,非食饵臂不放置食饵。实验组在非食饵臂末端放置加热装置作为错误惩罚外,其他条件与对照组相同。记录并比较实验大鼠于两种迷宫探索移动距离、时间、速度、进臂次数指标。结果与对照组相比,实验组大鼠移动距离和探索时间明显缩短且进入错误臂次数明显减少。结论使用改良电加热八臂迷宫实验方法能在训练7 d后更准确有效评估大鼠空间记忆学习能力,可作为一种有效的实验新方案。

关键词: 八臂迷宫, 学习记忆检测, 行为学, 动物实验

Abstract: Objective To identify a simple and effective experimental method for eight-arm maze study. Methods Twenty adult male SD rats were divided into a traditional method group (control group) and a modified electric heating pad method group (test group) (n=10). The bait was only placed at the end of feeding arm in control group. The test group had the same conditions as control group except that the heating pad was placed at the end of the non-predator arm as a punishment tool. Recorded and compared the moving distance, time, speed and the entrance times of rat in the arms via software. Results Compared with the control group, the test group rats shortened the movement distance and explored time significantly, and reduced the errors times into the wrong arm significantly as well. Conclusion Compared with the traditional method, the modified electric heating pad method can evaluate the spatial memory learning ability of rats after 7-days-training more accurately and effectively, which can be used as an effective experimental method.

Key words: Eight-arm maze, Spatial learning and memory, Behavior, Animal experiment

中图分类号:

Q95-33

刘波, 王杨, 李志杰. 应用于行为学研究中八臂迷宫实验方法的改良[J]. 实验动物与比较医学, 2019, 39(3): 226-230.

LIU Bo, WANG Yang, LI Zhi-jie. An Modified Eight-arm Maze Experiment Method in Behavioral Research[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 226-230.

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应用于行为学研究中八臂迷宫实验方法的改良

An Modified Eight-arm Maze Experiment Method in Behavioral Research

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