基于动物福利的雌性生殖发育毒性体外培养技术研究进展

doi:10.3969/j.issn.1674-5817.2019.02.016

摘要/Abstract

摘要： 生殖发育毒性研究的目的是通过动物试验检测外源物质对哺乳动物生殖功能和发育过程的影响,预测其可能产生的对生殖细胞、受孕、妊娠、分娩、哺乳等亲代生殖机能以及对子代胚胎/胎儿发育、出生后发育的不良影响。整体动物雌性生殖发育毒性研究方法一直广泛应用于药品、食品、化学品和化妆品的生殖发育毒性评价中,经过多年的验证并取得稳定的实验结果和结论。但它存在的主要问题是不敏感、周期长、所需受试样品量多,特别是由于体内实验生殖功能主要是以与生殖发育后果有关的参数作为基础,因此难以揭示毒性作用位点和毒性作用机制。雌性生殖发育毒理学替代方法指在生殖发育毒性评估中能尽可能替代动物实验、减少所需动物数量或使动物实验程序得以优化而减少动物痛苦的任何方法或程序,特别是在外源毒性物质的毒性初步评价上,可以作为整体动物实验的补充。本文将从雌性生殖系统细胞和组织培养、胚胎细胞和组织培养、乳腺细胞培养几大类总结雌性生殖发育毒性体外培养技术及研究进展,为雌性生殖发育毒性体外培养技术研究提供参考。

关键词: 雌性, 生殖发育毒性, 动物福利, 体外培养技术

Abstract: The aim of reproductive and developmental toxicity study is to identify the effects of exogenous substances on reproductive function and development of mammals by animal experiments, predicting the potential adverse effects on reproductive cells, pregnancy, pregnancy, delivery, lactation and other parental reproductive functions, as well as on the development of offspring embryos/fetuses and postnatal development. The methods of studying the reproductive and developmental toxicity of whole animal in females have been widely used in the evaluation of reproductive and developmental toxicity of drugs, food, chemicals and cosmetics. However, its main problems are insensitivity, long cycle, and large sample size. Especially, as the reproductive function of in vivo experiments is mainly based on parameters related to reproductive and developmental consequences, it is difficult to reveal the toxic action site and mechanism. Alternative method of female reproductive and developmental toxicology refers to any method or procedure which can replace animal experiments whenever possible, reduce the required number of animals or optimize and reduce animal suffering in animal experimental program, especially in preliminary evaluation of exogenous toxic substances as a powerful complement for animal experiments. In this paper, the technique and research progress of in vitro culture of female reproductive developmental toxicity were summarized in this paper, the advances in vitro culture of female reproductive toxicity were summarized from cells and tissue culture in female reproductive system, embryonic cells and tissue culture, breast cell culture so as to provide references for the study of in vitro culture of reproductive and developmental toxicity in females.

Key words: Female, Reproductive and development toxicity, Animal welfare, In vitro culture

中图分类号:

Q95-33

陆琴, 周跃华, 唐海飞, 王智文, 张婷. 基于动物福利的雌性生殖发育毒性体外培养技术研究进展[J]. 实验动物与比较医学, 2019, 39(2): 155-162.

LU Qin, ZHOU Yue-hua, TANG Hai-fei, WANG Zhi-wen, ZHANG Ting. Advances on in Vitro Culture of Female Reproductive and Development Toxicity Based on Animal Welfare[J]. Laboratory Animal and Comparative Medicine, 2019, 39(2): 155-162.

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