环境丰富对英国短毛猫应激相关指标的改善作用

doi:10.12300/j.issn.1674-5817.2021.130

摘要/Abstract

摘要：

目的　研究环境丰富对实验用英国短毛猫应激反应的缓解作用。方法　将14只实验用英国短毛猫按有无环境丰富干预措施随机分为福利组和对照组，每组7只，实验共持续11周。测定并分析比较各组实验猫的血液生理生化指标、神经内分泌指标、免疫学指标，评价不同组别的应激水平。结果　福利组的平均红细胞体积明显小于对照组（P＜0.05），淋巴细胞百分比极明显大于对照组（P＜0.01），中性粒细胞百分比极明显小于对照组（P＜0.01）。另外，福利组的尿素、肌酐、球蛋白和总蛋白水平均明显低于对照组（P＜0.05），肾上腺素、多巴胺两项指标也明显低于对照组（P＜0.05），而Ⅱ型干扰素（interferon-γ，IFN-γ）、白细胞介素（interleukin，IL）-1β、IL-2、IL-4、IL-5、IFN-γ/IL-4比值、IL-2/IL-4比值、IFN-γ/IL-5比值和IL-2/IL-5比值均高于对照组；其中IFN-γ、IL-2、IFN-γ/IL-5比值和IL-2/IL-5比值差异明显（P＜0.05），IL-5差异极明显（P＜0.01）。结论　采取环境丰富的干预措施能够有效提高实验猫的福利水平，缓解应激反应。

关键词: 实验动物福利, 环境丰富, 应激, 英国短毛猫

Abstract:

Objective To investigate the effect of environmental enrichment on alleviating stress in laboratory British-shorthair cats.Methods A total of fourteen laboratory British-shorthair cats were randomly divided into welfare group (n = 7) and control group (n = 7) according to the presence of environmental enrichment interventions which lasted for 11 weeks. The hematological and blood biochemical, neuroendocrine and immunological parameters of each group were measured and compared to evaluate the stress level of different groups.Results The mean corpuscular volume in the welfare group was significantly lower than that in the control group (P < 0.05), the percentage of lymphocyte was extremely higher than that in the control group (P < 0.01), and the neutrophilic percentage was extremely lower than that in the control group (P < 0.01). In addition, the levels of carbamide, creatinine, globulin, total protein in the welfare group were significantly lower than those in the control group (P < 0.05); the adrenaline and dopamine indicators in the welfare group were significantly lower than those in the control group (P < 0.05), while interferon-γ (IFN-γ), interleukin (IL)-1β, IL-2, IL-4, IL-5, IFN-γ/IL-4 ratio, IL-2/IL-4 ratio, IFN-γ/IL-5 ratio and IL-2/IL-5 ratio of the welfare group were higher than those of control group; among them, IFN-γ, IL-2, IFN-γ/IL-5 ratio and IL-2/IL-5 ratio were significantly different (P < 0.05), and IL-5 difference was extremely significant (P < 0.01).Conclusion The environmental enrichment interventions can effectively improve the welfare level of laboratory cats and reduce the biological costs of stress.

Key words: Laboratory animal welfare, Environmental enrichment, Stress, British-shorthair cats

中图分类号:

Q95-33

陈鸿婷, 杨斐, 胡樱. 环境丰富对英国短毛猫应激相关指标的改善作用[J]. 实验动物与比较医学, 2022, 42(2): 152-158.

Hongting CHEN, Fei YANG, Ying HU. Improving Effect of Environmental Enrichment on Stress-related Indicators of British-shorthair Cats[J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 152-158.

图/表 3

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检测指标	对照组（n=7）	福利组（n=7）
WBC ɷ/(10⁹·L^-1)	8.41±2.67	7.96±3.27
LYMPH ɷ/(10⁹·L^-1)	1.58±0.33	2.50±0.91
MONO ɷ/(10⁹·L^-1)	0.40±0.20	0.25±0.06
NEUT ɷ/(10⁹·L^-1)	5.82±2.21	4.34±2.04
EO ɷ/(10⁹·L^-1)	0.54±0.27	0.81±0.56
BASO ɷ /(10⁹·L^-1)	0.07±0.03	0.06±0.04
LYMPH %	20.09±5.37	32.41±6.36^**
MONO %	4.59±1.30	3.37±0.63
NEUT %	67.87±4.99	54.33±6.22^**
EO %	6.53±2.95	9.11±3.56
BASO %	0.93±0.58	0.77±0.36
HCT %	37.26±3.99	36.46±3.09
RBC ɷ/(10¹²·L^-1)	9.33±1.10	9.81±1.23
HGB ɷ/(g·L^-1)	12.70±1.31	12.54±0.94
RETIC ɷ/(10⁹·L^-1)	9.27±4.80	8.87±4.61
MCV ɷ/(fL)	40.01±2.10	37.34±1.88^*
MCHC ɷ/(g·L^-1)	34.11±0.83	34.44±0.65
MCH m/(pg)	13.64±0.88	12.89±0.81
PLT ɷ /(10⁹·L^-1)	312.86±92.76	211.57±74.30
MPV ɷ /(fL)	13.01±1.15	14.19±1.76
PCT %	0.40±0.10	0.30±0.11

检测指标	对照组（n=7）	福利组（n=7）
WBC ɷ/(10⁹·L^-1)	8.41±2.67	7.96±3.27
LYMPH ɷ/(10⁹·L^-1)	1.58±0.33	2.50±0.91
MONO ɷ/(10⁹·L^-1)	0.40±0.20	0.25±0.06
NEUT ɷ/(10⁹·L^-1)	5.82±2.21	4.34±2.04
EO ɷ/(10⁹·L^-1)	0.54±0.27	0.81±0.56
BASO ɷ /(10⁹·L^-1)	0.07±0.03	0.06±0.04
LYMPH %	20.09±5.37	32.41±6.36^**
MONO %	4.59±1.30	3.37±0.63
NEUT %	67.87±4.99	54.33±6.22^**
EO %	6.53±2.95	9.11±3.56
BASO %	0.93±0.58	0.77±0.36
HCT %	37.26±3.99	36.46±3.09
RBC ɷ/(10¹²·L^-1)	9.33±1.10	9.81±1.23
HGB ɷ/(g·L^-1)	12.70±1.31	12.54±0.94
RETIC ɷ/(10⁹·L^-1)	9.27±4.80	8.87±4.61
MCV ɷ/(fL)	40.01±2.10	37.34±1.88^*
MCHC ɷ/(g·L^-1)	34.11±0.83	34.44±0.65
MCH m/(pg)	13.64±0.88	12.89±0.81
PLT ɷ /(10⁹·L^-1)	312.86±92.76	211.57±74.30
MPV ɷ /(fL)	13.01±1.15	14.19±1.76
PCT %	0.40±0.10	0.30±0.11

检测指标	对照组（n=7）	福利组（n=7）
ALB ρ/ (g·L^-1)	33.14±1.46	33.29±1.03
ALKP z/ (U·L^-1)	43.00±10.53	42.86±7.41
ALT z/ (U·L^-1)	70.00±19.38	60.29±9.28
UREA c/ (mmol ·L^-1)	6.50±1.26	5.06±0.95^*
CA c/ (mmol·L^-1)	2.40±0.07	2.34±0.07
CHOL c/ (mmol·L^-1)	2.48±0.53	2.65±0.57
CREA c/ (μmol·L^-1)	120.00±16.24	102.29±7.28^*
GLOB ρ/ (g·L^-1)	50.43±6.21	43.71±2.71^*
GLU c/ (mmol·L^-1)	6.17±1.08	5.43±0.55
PHOS c/ (mmol·L^-1)	1.73±0.12	1.68±0.17
TBIL c/ (μmol·L^-1)	3.14±1.25	2.14±0.35
TP ρ/ (g·L^-1)	83.45±5.53	77.14±2.80^*

检测指标	对照组（n=7）	福利组（n=7）
ALB ρ/ (g·L^-1)	33.14±1.46	33.29±1.03
ALKP z/ (U·L^-1)	43.00±10.53	42.86±7.41
ALT z/ (U·L^-1)	70.00±19.38	60.29±9.28
UREA c/ (mmol ·L^-1)	6.50±1.26	5.06±0.95^*
CA c/ (mmol·L^-1)	2.40±0.07	2.34±0.07
CHOL c/ (mmol·L^-1)	2.48±0.53	2.65±0.57
CREA c/ (μmol·L^-1)	120.00±16.24	102.29±7.28^*
GLOB ρ/ (g·L^-1)	50.43±6.21	43.71±2.71^*
GLU c/ (mmol·L^-1)	6.17±1.08	5.43±0.55
PHOS c/ (mmol·L^-1)	1.73±0.12	1.68±0.17
TBIL c/ (μmol·L^-1)	3.14±1.25	2.14±0.35
TP ρ/ (g·L^-1)	83.45±5.53	77.14±2.80^*

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