阴道炎动物模型制备方法研究进展

doi:10.3969/j.issn.1674-5817.2016.04.015

摘要/Abstract

摘要： 阴道炎是由病原体侵入而引起的女性常见感染性疾病,临床上常见于阴道黏膜或宫颈损伤(如分娩、流产或手术等)后,其发病机制至今尚未完全明确。借助人类疾病动物模型开展人类疾病相关研究,是阐明疾病的发病机制或药物疗效的重要手段。建立稳定可靠的阴道炎动物模型不仅为探索人类阴道炎的发病机制和研究病理生理改变提供重要材料,也对新型药物和疫苗开发具有促进作用。按照感染类型,阴道炎动物模型可分为细菌性阴道炎(BV)动物模型、霉菌性阴道炎(VVC)动物模型和滴虫性阴道炎(TV)动物模型三大类。其中细菌性感染模型主要是由阴道加德纳菌、奈瑟双球菌感染、肠埃希菌感染模型或金黄色葡萄球菌感染所致; VVC动物模型主要由白念珠菌或光滑念珠菌感染所致; TV动物模型主要由阴道毛滴虫以及牛胎毛滴虫感染所致。该模型所选择的动物主要有小鼠、大鼠、家兔、非人灵长类。此外,猪阴道黏膜、人类上皮细胞也常用于阴道炎的模型制备。

关键词: 阴道炎, 动物模型, 制备方法, 进展

Abstract: Vaginitis is the common infectious diseases of the women caused by invasive pathogens, which is often happened after vaginal mucosa or cervical injury (such as childbirth, abortion, surgery, etc.). However, the etiology and pathogenesis of vaginitis is still not entirely clear yet. Using animal models of human diseases to research human diseases is an important mean to clarify the pathogenesis of the disease or drug efficacy. Establishing a stable and reliable animal model of vaginitis is an important method for reseaching the pathogenesis and pathophysiology changes of human vaginitis and have an important role in promoting the development of new drugs and vaccines. There are three types of animal models of vaginitis: bacterial vaginosis, vulvovaginal candidiasis and trichomonas vaginitis according to the type of infection. The animal models of bacterial vaginosis are mainly infected by Gardnerella vaginalis, Neisseria gonorrhoeae, Escherichia coli or Staphylococcus aureus. The animal models of vulvovaginal candidiasis are mainly infected by Candida albicans or Candida glabrata. The animal models of trichomonas vaginitis are mainly infected by Trichomonas vaginalis or Tritrichomonas foetus. Mice, rats, rabbits and non-human primates are mainly selected to prepare animal models of vaginosis. In addition, pig vaginal mucosa and human epithelial cells are also commonly used in the preparation of animal models of vaginosis.

Key words: Vaginitis, Animal model, Preparation method, Progress

中图分类号:

R711Q95-33

李冬冬, 庞琴霞, 朱敏, 康乐. 阴道炎动物模型制备方法研究进展[J]. 实验动物与比较医学, 2016, 36(4): 317-322.

LI Dong-dong, PANG Qin-xia, ZHU Min, KANG Le. Progress on Methods for Establishing Animal Model of Vaginitis[J]. Laboratory Animal and Comparative Medicine, 2016, 36(4): 317-322.

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