ZDF与SD大鼠动脉粥样硬化形成及其磷脂酶A2表达的比较研究

doi:10.3969/j.issn.1674-5817.2015.01.003

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (1): 10-16.DOI: 10.3969/j.issn.1674-5817.2015.01.003

• 华东地区第十三届实验动物科学学术交流会优秀论文选刊 • 上一篇下一篇

ZDF与SD大鼠动脉粥样硬化形成及其磷脂酶A2表达的比较研究

斯徐伟¹, 奚赛飞², 徐孝平², 诸葛福媛¹, 陈小真², 蔡月琴², 陈民利²

1.绍兴市人民医院, 绍兴 312000;
2.浙江中医药大学动物实验研究中心比较医学研究中心,杭州 310053

收稿日期:2014-06-30 出版日期:2015-02-25 发布日期:2015-02-25
作者简介:斯徐伟(1963-), 男, 主任医师, 研究方向:内分泌代谢。 E-mail:sixuwei@medmail.com.cn
基金资助:
浙江省公益性技术应用研究项目(2013C37021); “浙江省卫生高层次创新人才培养工程项目”

Comparative Study on Formation of Atherosclerosis and Phospholipase A2 Expression in ZDF and SD Rats

SI Xu-wei¹, XI Sai-fei², XU Xiao-ping², ZHUGE Fu-yuan¹, CHEN Xiao-zhen², CAI Yue-qin², CHEN Min-li²

1. Shaoxing People’s Hospital, Shaoxing 312000, China;
2. Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China

Received:2014-06-30 Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的观察ZDF和SD大鼠动脉粥样硬化(atherosclerosis, AS)形成和AS组织中磷脂酶A2(cPLA2、sPLA2、LP-PLA2)的表达情况。方法取ZDF(fa/fa)大鼠和SD大鼠各6只,用高脂饲喂加小剂量多次腹腔注射7 ×10⁵ U/kg维生素D3注射液,建立ZDF大鼠AS模型组和SD大鼠AS模型;另取ZDF(fa/+)大鼠和SD大鼠各6只,分别作为ZDF大鼠对照组和SD大鼠对照组,饲喂普通饲料。检测造模前后GLU、TC、TG、HDL-C、LDL-C的含量和造模后血清Ca²⁺含量,并取AS大鼠腹主动脉进行HE染色和cPLA2、sPLA2、LP-PLA2的mRNA表达检测。结果 ZDF大鼠AS模型组和SD大鼠AS模型组的空腹血糖、血脂水平和血清Ca²⁺含量均显著升高(P<0.01),腹主动脉出现典型的AS并伴明显的钙化,ZDF大鼠模型组血糖、血脂明显高于SD大鼠模型组,其AS病变也相对严重;ZDF大鼠AS模型组cPLA2和sPLA2的表达显著升高(P<0.01),LP-PLA2的表达显著降低(P<0.01); 而SD大鼠AS模型组cPLA2、sPLA2、LP-PLA2的表达均显著升高(P<0.01)。结论 ZDF大鼠和SD大鼠进行高脂饲喂加小剂量多次注射维生素D3均能建立AS模型,ZDF(fa/fa)大鼠的AS病变相对严重,与其高血糖和高血脂状态有关;ZDF大鼠和SD大鼠中的PLA2的表达存在差异,这可能与SD大鼠AS形成的分子病理机制有关。

关键词: ZDF大鼠, SD大鼠, 高脂饲喂, 维生素D3, 动脉粥样硬化(AS), 磷脂酶A2(PLA2)

Abstract: Objective To observe the formation of Atherosclerosis(AS) and expression of cPLA2, sPLA2, LP-PLA2 in AS tissue of ZDF rats and SD rats. Methods 6 ZDF (fa/fa) rats and 6 SD rats, with high-fat feeding and small dose, multiple times, intraperitoneal injection of 7×10⁵ U/kg vitamin D3,to build ZDF rats AS model group and SD rats AS model group, 6 ZDF (fa/+) rats and 6 SD rats, respectively,as ZDF rats control group and SD rats control group, fed with normal diet. GLU, TC, TG, HDL-C, LDL-C were measured before and after the establishment of the model, blood Ca²⁺ was measured after the establishment of the model, HE staining and Real-time PCR to detect the expression of cPLA2, sPLA2, LP-PLA2 mRNA in the abdominal aorta. Results After the establishment of the model, fasting blood glucose, blood lipid and blood Ca²⁺ were significantly higher (P<0.01), typical AS and obvious calcification were appeared in abdominal aorta of ZDF rats AS model group and SD rats AS model group, fasting blood glucose, blood lipid of ZDF rats AS model group were significantly higher than SD rats AS model group, and AS lesion was relatively serious, in ZDF rats AS model group, the expression of cPLA2, sPLA2 were significantly increased (P<0.01), while the expression of LP-PLA2 was significantly decreased (P<0.01); in SD rats AS model group, the expression of cPLA2, sPLA2, LP-PLA2 were significantly increased (P<0.01). Conclusions With high-fat feeding and small dose, multiple times, injection of vitamin D3 can establish the AS model in ZDF rats and SD rats, and AS lesion is relatively serious in ZDF rats, the expression of PLA2s are different in ZDF rats and SD rats, and this may be related to molecular pathological mechanism of the formation of AS in ZDF rats and SD rats.

Key words: ZDF RAT, SD rat, High fat feeding, Vitamin D3, Atherosclerosis (AS), PLA2

中图分类号:

Q95-33

斯徐伟, 奚赛飞, 徐孝平, 诸葛福媛, 陈小真, 蔡月琴, 陈民利. ZDF与SD大鼠动脉粥样硬化形成及其磷脂酶A2表达的比较研究[J]. 实验动物与比较医学, 2015, 35(1): 10-16.

SI Xu-wei, XI Sai-fei, XU Xiao-ping, ZHUGE Fu-yuan, CHEN Xiao-zhen, CAI Yue-qin, CHEN Min-li. Comparative Study on Formation of Atherosclerosis and Phospholipase A2 Expression in ZDF and SD Rats[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 10-16.

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ZDF与SD大鼠动脉粥样硬化形成及其磷脂酶A2表达的比较研究

Comparative Study on Formation of Atherosclerosis and Phospholipase A2 Expression in ZDF and SD Rats

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