阿胶对环磷酰胺所致血虚证模型小鼠毛发生长及发质影响的研究

doi:10.3969/j.issn.1674-5817.2015.01.004

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (1): 17-22.DOI: 10.3969/j.issn.1674-5817.2015.01.004

• 华东地区第十三届实验动物科学学术交流会优秀论文选刊 • 上一篇下一篇

阿胶对环磷酰胺所致血虚证模型小鼠毛发生长及发质影响的研究

李浩¹, 籍国霞^2,3, 王可洲¹, 嵇传良^2,3, 李兰波¹, 张燕¹, 冯墨竹¹, 李建梅¹, 刘涛¹

1.山东省医学科学院山东省实验动物中心,济南 250002;
2.山东东阿阿胶集团股份有限公司,东阿 252200;
3.国家胶类中药工程技术研究中心,东阿 252200

收稿日期:2014-06-30 出版日期:2015-02-25 发布日期:2015-02-25
作者简介:李浩(1982-), 男, 技师, 研究方向:实验动物毒理病理学及动物实验;E-mail:lewesd@163.com
基金资助:
国家重大新药创制(No.2011ZX09201-201-10)

The Influence of Colla Corii Asini on Growth and Quality of Hair in Mice Treated by Cyclophosphamide

LI Hao¹, JI Guo-xia^2,3, WANG Ke-zhou¹, JI Chuan-liang^2,3, LI Lan-bo¹, ZHANG Yan¹, FENG Mo-zhu¹, LI Jian-mei¹, LIU Tao¹

1. Shandong Laboratory Animal Center of Shandong Academy of Medical Sciences, Shandong Jinan 250002, China;
2. Shandong Dong-e E-jiao Co. Ltd., Shandong Donge 252200, China;
3. National Engineering Technology Research Center of Rubber Medicine, Shandong Donge 252200, China

Received:2014-06-30 Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的探究阿胶对环磷酰胺(CTX)所致血虚证C57BL/6小鼠毛发生长及质量改善的作用。方法将CTX所致血虚证模型C57BL/6小鼠分为阿胶给药组(高、中、低剂量组)和模型对照组,另设空白对照组,分别灌胃相应剂量的阿胶溶液和生理盐水,连续20 d; 首次给药后0 d,12 d和20 d,测定小鼠新生毛发的长度、氨基酸含量、毛囊数量及血液学参数,并用电子显微镜观察毛发的微观形态。结果阿胶给药组及空白对照组白细胞总数、淋巴细胞含量和单核细胞含量均高于模型对照组; 中、高剂量组小鼠毛发中氨基酸含量高于模型对照组; 首次给药后20 d,小鼠新生毛发长度及浓密程度: 空白对照组>阿胶给药组(高、中、低剂量组)>模型对照组; 电镜观察,各组小鼠新生毛发直径存在明显差异：空白对照组>高剂量组>中、低剂量组>模型对照组; 小鼠新生毛囊数量: 空白对照组>阿胶给药组>模型对照组。结论阿胶可以升高CTX所致血虚证模型C57BL/6小鼠体内白细胞数量,促进C57BL/6贫血小鼠皮肤新生毛囊的形成,促进毛发生长,增加毛发中氨基酸含量,起到改善新生毛发质量的作用。

关键词: 阿胶, 环磷酰胺, 血虚证模型, 毛发生长, 改善发质

Abstract: Objective To explore the effects of colla corii asini on the growth and quality improvement of hair in mice of anemia caused by cyclophosphamide. Methods The mice model of anemia was established by ip cyclophosphamide. C57BL/6 mice were divided into five groups (the high dose group, the medium group and the low dose group, the model control group and the blank control group), and the different dose of colla corii asini or physiological saline were respectively administrated for 20 days by gavage. The number of hair follicles, the various content of blood cell, the length and the amino acid content of the hair in mice were determined, and the microstructure of hair were observed by the electron microscope at the 0 day, 12 days and 20 days after the first treatment. Results The contents of white blood cells, lymphocytes and monocytes in all colla corii asini groups and the blank control group, were higher than that of the model control group. The amino acids in the new hair of medium and high dose group mice were higher than that of the model control group. At the 20 days after the first treatment, the length and thickness of new hair in mice of all colla corii asini groups (the high, medium and low dose group) was more than that of the model control group, and was less than that of the blank control group, but there was no significant difference among the hair length (P>0.05). Scanning electron microscope (SEM) images showed there were significant differences in new hair diameter among different groups, the diameter of all colla corii asini groups was more than that of the model control group, and was less than that of the blank control group. And the diameter of the high dose group was more than that of the medium and the low dose group. The number of hair follicle of all colla corii asini groups was more than that of the model control group, and was less than that of the blank control group. Conclusion The colla corii asini could increase the number of white blood cells in C57BL/6 mice of anemia caused by cyclophosphamide, promote the formation of new hair follicles in C57BL/6 mice, promote hair growth, increase the content of amino acid and improve the quality of new grown hair.

Key words: Colla corii asini, Cyclophosphamide, Model of blood deficiency, Hair growth, Quality improvement of hair

中图分类号:

Q95-33

李浩, 籍国霞, 王可洲, 嵇传良, 李兰波, 张燕, 冯墨竹, 李建梅, 刘涛. 阿胶对环磷酰胺所致血虚证模型小鼠毛发生长及发质影响的研究[J]. 实验动物与比较医学, 2015, 35(1): 17-22.

LI Hao, JI Guo-xia, WANG Ke-zhou, JI Chuan-liang, LI Lan-bo, ZHANG Yan, FENG Mo-zhu, LI Jian-mei, LIU Tao. The Influence of Colla Corii Asini on Growth and Quality of Hair in Mice Treated by Cyclophosphamide[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 17-22.

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阿胶对环磷酰胺所致血虚证模型小鼠毛发生长及发质影响的研究

The Influence of Colla Corii Asini on Growth and Quality of Hair in Mice Treated by Cyclophosphamide

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