实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (4): 301-305.DOI: 10.12300/j.issn.1674-5817.2022.023

• 动物实验技术与方法专题 • 上一篇    下一篇

放置时间对大鼠血清样本中电解质含量及pH值的影响

冯丁山()(), 黄业宇, 张晓昕, 吴爱琴, 王湛, 苏林梁   

  1. 海南省疾病预防控制中心, 海口 570203
  • 收稿日期:2022-03-03 修回日期:2022-05-25 出版日期:2022-08-25 发布日期:2022-09-01
  • 作者简介:冯丁山(1975—),男,本科,副主任医师,研究方向:食品毒理、实验动物管理。E-mail: fds1369@163.com。 ORCID: 0000-0003-0488-7684
  • 基金资助:
    海南省卫生健康行业科研项目“食品安全评价中大鼠电解质指标检测系统的建立”(20A200123)

Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples

Dingshan FENG()(), Yeyu HUANG, Xiaoxin ZHANG, Aiqin WU, Zhan WANG, Linliang SU   

  1. Hainan Provincial Center for Disease Control and Prevention, Haikou 570203, China
  • Received:2022-03-03 Revised:2022-05-25 Online:2022-08-25 Published:2022-09-01
  • Contact: FENG Dingshan (ORCID: 0000-0003-0488-7684), E-mail: fds1369@163.com

摘要:

目的 观察SD大鼠血清样本放置时间对血清中K+、Na+、Ca2+、Cl-含量和pH值检测结果的影响。 方法 取雌雄SD大鼠各20只,腹腔静脉采血后分离血清,样本加盖存放于2~8 ℃,在0 h、2 h、4 h、6 h、24 h、48 h和120 h共7个时间点分别测定K+、Na+、Ca2+、Cl-含量和pH值。 结果 K+、Na+ 含量各时间点比较差异无统计学意义(P>0.05);与0 h检测结果比较,放置48 h和120 h的Cl- 含量明显升高(P<0.05),放置48 h和120 h的Ca 2+含量明显降低(P<0.05),放置24 h、48 h、120 h的pH值明显升高(P<0.05);而在6 h内这3个指标均无明显差异(P>0.05)。 结论 SD大鼠血清样本放置时间对血清中Ca2+、Cl-、pH检测结果有明显影响,样本应加盖贮存于2~8 ℃,并在6 h内完成检测。

关键词: 血清, 放置时间, K+, Na+, Ca2+, pH值, SD大鼠

Abstract:

Objective To observe the effects of serum sample placement time on the detection results of K +, Na+, Ca2+, Cl - and pH in sera of SD rats. Methods The sera of 20 male and 20 female SD rats were separated after blood collection from abdominal vein. The samples were covered and stored in a refrigerator at 2-8 ℃. The electrolyte indexes including K+, Na+, Ca2+, Cl- and pH in serum were measured at 7 time points of 0 h, 2 h, 4 h, 6 h, 24 h, 48 h and 120 h after storage. Results There were no significant differences in the detection results of K+ and Na+ at each time point (P > 0.05). Compared with the test results at 0 h, the content of Cl- increased significantly at 48 h and 120 h (P < 0.05); the content of Ca2+ decreased significantly at 48 h and 120 hours, and the pH increased significantly at 24 h, 48 h and 120 h (P < 0.05). There was no significant difference in these three indexes within 6 h (P > 0.05). Conclusion The storage time of serum samples of SD rats has a significant impact on the detection results of Ca2+, Cl- and pH in serum. The samples should be covered and stored in a refrigerator at 2-8 ℃ and completed within 6 h after sample preparation.

Key words: Serum, Placement time, K+, Na+, Ca2+, pH, Sprague-Dawley rats

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