脾胃虚寒型胃溃疡动物模型研究进展

doi:10.12300/j.issn.1674-5817.2025.015

实验动物与比较医学

• •

脾胃虚寒型胃溃疡动物模型研究进展

刘子琪¹(), 李云英¹()(), 李钦¹^,², 李元涵¹, 何芳雁¹^,²()(), 温伟波¹^,²()

^1.云南中医药大学中药学院, 昆明 650500
^2.云南省中西医结合慢病防治重点实验室, 昆明 650500

收稿日期:2025-01-24 修回日期:2025-06-10 出版日期:2025-08-15
通讯作者: 何芳雁（1989—），女，硕士，高级实验师，研究方向：中药药理与应用。E-mall： hefangyan@foxmail.com。ORCID： 0009-0009-8945-8002；
温伟波（1968—），男，硕士，教授，研究方向：中药民族药治疗代谢性疾病及新突发传染病研究。E-mall： wenweibo2020@163.com。ORCID： 0009-0009-9759-3765；
作者简介:刘子琪（2000—），女，硕士研究生，研究方向：中药药理与应用。E-mall： 1947316311@qq.com。ORCID：0009-0003-1432-1657；
李云英（1997—），女，硕士在读，研究方向：中药药理与应用。E-mall： 2594903717@qq.com。ORCID：0009-0009-3030-237X；
基金资助:
国家重点研发计划“中医证候动物模型的建立及其在中药/民族药评价中的应用研究”(2023YFF0724803)

Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type

LIU Ziqi¹(), LI Yunying¹()(), LI Qin¹^,², LI Yuanhan¹, HE Fangyan¹^,²()(), WEN Weibo¹^,²()

^1.College of Traditional Chinese Medicine, Yunnan University of Chinese Medicine, Kunming 650500, China
^2.Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease Prevention and Control, Kunming 650500, China

Received:2025-01-24 Revised:2025-06-10 Published:2025-08-15
Contact: HE Fangyan (ORCID: 0009-0009-8945-8002), E-mall: hefangyan@foxmail.com;
WEN Weibo (ORCID: 0009-0009-9759-3765), E-mall:wenweibo2020@163.com;

摘要/Abstract

摘要：

胃溃疡（gastric ulcer，GU）是消化系统的常见病、多发病和顽固性疾病之一。脾胃虚寒是GU最常见且难以治愈的一种证型，是医学研究领域的重点和难点。因此，构建科学合理且符合临床实际的脾胃虚寒型GU动物模型并制定客观有效的评价标准，对于深入研究GU的发病机制和治疗方法具有重要意义。本文通过系统梳理相关文献，对脾胃虚寒型GU动物模型的制备方法进行了全面介绍：首先介绍西医GU病理模型的构建方法，如幽门结扎法、水浸-束缚应激法、乙醇诱导法、乙酸诱导法等；阐述中医脾胃虚寒证候模型的建立方法，包括饮食失节法、苦寒泻下法、过度劳倦法、耗气破气法和过食酸味法等；重点讲述脾胃虚寒型GU病证结合模型的构建方法，包括双因素造模法和三因素造模法。同时，从多个角度总结了脾胃虚寒型GU动物模型的评价指标，包括动物体征表征（外观症状、动物行为和代谢指标）、组织形态与分子生物学指标（胃功能、氧化应激、炎症因子、其他细胞因子、凝血4项、肠道菌群检测），力求构建一个综合性的评价体系。本文进一步从方药反证的角度，通过分析方剂的药物组成和药理作用效果，推断其治疗的疾病动物模型所属的证型，从而验证动物模型是否构建成功。通过本文综述，以期为构建与临床实际高度契合、体现中医药特色的GU中医证候动物模型提供有力参考，从而推动GU中医证候研究和中医药治疗GU方法的深入探索，促进中医药在GU治疗中的应用和发展。

关键词: 胃溃疡, 脾胃虚寒, 病证结合, 动物模型

Abstract:

Gastric ulcer (GU) is one of the common, frequently-occurring and intractable diseases of the digestive system. Spleen-stomach deficiency cold type is the most common and hard-to-cure syndrome pattern of GU, and is both a focus and a challenge in medical research. Therefore, constructing a scientific, reasonable, and clinically practical animal model of GU with spleen-stomach deficiency cold type and formulating objective and effective evaluation criteria are of great significance for in-depth research on the pathogenesis and treatment of GU. In this paper, the methods for constructing GU animal models of spleen-stomach deficiency cold type are comprehensively introduced by systematically reviewing the relevant literature. Firstly, the construction methods of pathological models of GU in Western medicine are introduced, including pyloric ligation method, water immersion-restraint stress method, ethanol-induced method, acetic acid-induced method, etc. This paper expounds the establishment methods for spleen-stomach deficiency cold syndrome type model in traditional Chinese medicine (TCM), including diet disorder method, bitter cold diarrhea method, excessive fatigue method, Qi consumption and Qi impairment method, and overeating sour-flavor method. This paper focuses on the construction methods for disease-syndrome combination GU models of spleen-stomach deficiency cold type, including two-factor modeling method and three-factor modeling method. Meanwhile, the evaluation indices of GU animal models of spleen-stomach deficiency cold type were summarized from various respects, including animal physical signs ( appearance symptoms, animal behavior, and metabolic indices), and tissue morphology and molecular biological indicators ( gastric function, oxidative stress, inflammatory factors, other cytokines, four coagulation parameters, intestinal flora detection ), for constructing a comprehensive evaluation system. From the perspective of prescription-based verification, this paper further analyzes the drug composition and pharmacological effects to infer the syndrome type of the treated animal model, so as to verify whether the target animal model is successfully constructed. This review aims to provide a valuable reference for establishing a syndrome-specific GU animal model that closely aligns with clinical reality and embodies the principles of Chinese medicine. This will further advance research on TCM-pattern GU syndromes and deepen the exploration of herbal medicine-based treatments for GU, ultimately promoting the clinical integration and advancement of Chinese medicine in GU therapy.

Key words: Gastric ulcer, Spleen-stomach deficiency cold, Disease-syndrome combination, Animal model

中图分类号:

R-332

刘子琪,李云英,李钦,等.脾胃虚寒型胃溃疡动物模型研究进展[J]. 实验动物与比较医学.. DOI: 10.12300/j.issn.1674-5817.2025.015.

LIU Ziqi,LI Yunying,LI Qin,et al. Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2025.015.

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脾胃虚寒型胃溃疡动物模型研究进展

Research Progress on Animal Models of Gastric Ulcer of Spleen-Stomach Deficiency Cold Type

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