成年兔桡骨骨缺损模型的骨缺损长度探讨

doi:10.3969/j.issn.1674-5817.2018.03.004

摘要/Abstract

摘要： 目的建立成年兔桡骨不同尺寸骨缺损模型,确定成年兔桡骨临界性骨缺损长度。方法选取8月龄雄性新西兰兔18只,随机分为3组,根据骨缺损长度记为A、B和C组,缺损长度依次为 10 mm、12 mm和15 mm, 每组6只,行双侧手术,共计12侧。分别于术后当日,术后4周、8周和12 周行 CT检查,应用CT-Hedberg评分评价骨缺损愈合情况。于术后 12 周处死实验兔,取出尺桡骨标本,通过大体观察及HE染色组织学观察分析缺损区骨愈合情况。结果术后所有兔均存活,术后12周大体观察见A组中12例(100%)桡骨断端均有连续性骨痂生成,新生骨表面光滑,塑形良好。B组中11例(91.7%)桡骨断端有连续性骨痂生成。C组缺损断端髓腔封闭,缺损区由纤维组织填充。CT图像显示术后4周C组出现5例尺骨骨折现象,骨折侧未计入统计,12周CT图像可见A组和B组完全桥接的桡骨已形成再通的髓腔,C组断端及缺损尺侧仅有少量新骨生成,髓腔封闭。术后各时间点CT-Hedberg评分: C组(1.14±0.38, 1.29±0.49, 1.57±0.53)明显低于A组(2.42±0.51, 3.17±0.58, 3.75±0.45)和B组(2.25±0.45, 2.67±0.78, 3.50±0.67),(P<0.05), 但A组与B组间差异无统计学意义(P>0.05)。组织学结果示A组和B组缺损已修复完全,髓腔再通,C组缺损区由纤维组织填充。结论在进行成年兔骨缺损研究时,可选择8月龄雄性新西兰兔桡骨构建15 mm长骨缺损,但同时应注意由于体质量较大带来的易骨折等并发症。

关键词: 成年兔, 桡骨, 临界性骨缺损, 缺损长度, 动物模型

Abstract: Objective To establish experimental models with different defect lengths of adult rabbit radius and to determine the optimal length of bone critical-size defects in adult rabbits.Methods Eighteen male New Zealand White rabbits aged 8 months were included in this study.They were randomly assigned into 3 groups,according to the defect lengths of radius:group A (10 mm),group B (12 mm) and group C (15 mm).There were 6 rabbits with a total of 12 sides in each group.These rabbits underwent bilateral radial surgery.Computed tomography (CT) scans were taken immediately after the surgery and at 4-week intervals,respectively.The healing of the bone defect was evaluated with the CT-Hedberg scores.The rabbits were sacrificed at 12 weeks postoperatively and the forearms were harvested for gross and histological observations of new bone formation.Results All rabbits survived after the operation.Twelve (100%) of the radial bones in group A and eleven (91.7%) in group B revealed continuous bridged callus with a smooth surface.In contrast,the defect area was filled with fibrous tissue in group C with the closure of both ends.CT images showed that five out of twelve sides in group C appeared ulnar fracture at fourth weeks postoperatively.We excluded the fracture sides out of the statistical analysis.At twelve weeks postoperatively,recanalized marrow cavity of the bridged radius in group A and group B were observed on CT images.However,a small amount of callus along with closed marrow cavity was found at both ends in group C.Based on the CT-Hedberg scoring system,we found that the scores of group C (1.14±0.38,1.29±0.49,1.57±0.53) were lower than group A (2.42±0.51,3.17±0.58,3.75±0.45) and group B (2.25±0.45,2.67±0.78,3.50±0.67),respectively.There were significant statistical differences among the three groups at each time point postoperatively (P<0.05).However,there were lacks of significant differences at various postoperative time points between group A and group B (P>0.05).Histological analysis showed that the bone defects in group A and group B were perfectly repaired with the recanalization of marrow cavity.It was observed that in group C the ends of the defects were closed and the defect area was filled with fibrous tissue.Conclusions Eight-month-old male New Zealand White rabbits could be selected when radius defect in adult rabbit was established and 15 mm was considered the most appropriate critical-size defect length.However,it should be taken into account that fractures would be one of the complications at such a age of rabbits with heavier weight.

Key words: Adult rabbit, Radius, Critical-size bone defect, Defect length, Animal model

中图分类号:

Q95-33

宋子鉴, 郑欣, 王进, 张星晨, 郭开今. 成年兔桡骨骨缺损模型的骨缺损长度探讨[J]. 实验动物与比较医学, 2018, 38(3): 182-187.

SONG Zi-Jian, ZHENG Xin, WANG Jin, ZHANG Xing-Chen, GUO Kai-Jin. Investigation on Defect Length in a Model of Radius Critical-size Defect in Adult Rabbits[J]. Laboratory Animal and Comparative Medicine, 2018, 38(3): 182-187.

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