脑心通胶囊对氯胺酮成瘾大鼠脑细胞损伤的保护作用

doi:10.3969/j.issn.1674-5817.2019.06.003

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (6): 437-442.DOI: 10.3969/j.issn.1674-5817.2019.06.003

脑心通胶囊对氯胺酮成瘾大鼠脑细胞损伤的保护作用

柳小波^1,4, 杨梅², 宋佳³

1.武汉科技大学医学院公共卫生系, 武汉 430065;
2.武汉科技大学医学院儿少卫生与妇幼保健学教研室, 武汉 430065;
3.华中科技大学同济医学院附属武汉精神卫生中心中医病区, 武汉 430022;
4.华中科技大学同济医学院附属武汉精神卫生中心精神科, 武汉 430022

收稿日期:2019-04-29 出版日期:2019-12-25 发布日期:2021-01-05
作者简介:柳小波(1984-), 男, 主治医师, 研究方向: 精神医学及心理学。E-mail: 78612717@qq.com
基金资助:
武汉市卫生和计划生育委员会科研项目(WX18D31)

Protective Effect of Naoxintong Capsule on Brain Cell Injury in Rats with Ketamine Addiction

LIU Xiao-bo^1,4, YANG Mei², Song Jia³

1. Wuhan University of Science and Technology Medical College, Department of Public Health, Wuhan 430065, China;
2. Wuhan University of Science and Technology Medical College, Department of Maternal and Child Health Education and Research, Wuhan 430065, China;
3. Wuhan Mental Health Center Affiliated to Tongji Medicine, Huazhong University of Science and Technology, Traditional Chinese Medicine Disease Area, Wuhan 430022, China;
4. Wuhan Mental Health Center Affiliated to Tongji Medicine, Huazhong University of Science and Technology, Psychiatry, Wuhan 430022, China

Received:2019-04-29 Online:2019-12-25 Published:2021-01-05

摘要/Abstract

摘要： 目的探讨脑心通胶囊对氯胺酮成瘾大鼠脑细胞损伤的保护作用。方法将60只SD大鼠随机分为对照组、模型组、脑心通胶囊高、中、低剂量组和阳性对照组各10只。模型组、脑心通胶囊组和阳性对照组经腹腔注射浓度为50 mg/mL的盐酸氯胺酮注射液(10 mg/kg),脑心通胶囊高、中、低剂量组灌胃脑心通胶囊(500 mg/kg、250 mg/kg和100 mg/kg),阳性对照组腹腔注射l-四氢巴马汀(20 mg/kg),1次/d,连续7 d。实验前后测试每只大鼠的条件位置偏爱(CPP),实验后,采用免疫组织化学检测脑组织即刻早期基因(c-fos)的表达,采用Western blotting测定脑组织中B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白水解酶(Caspase-3)蛋白的表达,HE染色观察脑组织病灶的病理变化。结果与对照组相比,模型组、脑心通胶囊高、中、低剂量组和阳性对照组大鼠CPP值明显升高(P<0.05),脑组织c-fos基因和Caspase-3、Bax蛋白表达量明显升高(P<0.05),Bcl-2表达量明显降低(P<0.05); 与模型组相比,脑心通胶囊高、中、剂量组和阳性对照组大鼠CPP值明显降低(P<0.05),脑组织c-fos基因和Caspase-3、Bax蛋白表达量明显下降(P<0.05),Bcl-2表达量明显升高(P<0.05),HE染色显示脑细胞病变程度减轻。结论脑心通胶囊可能通过调节脑组织的细胞凋亡,保护氯胺酮成瘾大鼠的脑细胞损伤。

关键词: 脑心通胶囊, 氯胺酮, 大鼠, 脑细胞损伤

Abstract: Objective To investigate the protective effect of naoxintong capsule on brain cell injury in rats with ketamine addiction. Methods Sixty SD rats were randomly divided into control, model, Naoxintong capsule with high, medium and low dose, and positive control group, with 10 rats in each group. The model group, Naoxintong capsule high, medium and low dose group and positive control group was intraperitoneally injected with 50 mg/mL ketamine hydrochloride injection (10 mg/kg). Naoxintong Capsule high, medium and low dose group was gavaged with Naoxintong Capsule (500 mg/kg, 250 mg/kg and 100 mg/kg). Positive control group was intraperitoneally injected with L-tetrahydropalmatine (20 mg/kg). Control group was intraperitoneally injected with same amount of normal saline, once a day for 7 days. The conditional position preference (CPP) of each rat was detected before and after the experiment. After experiment, expression of brain tissue c-fos gene was detected by immunohistochemistry. The expression of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteinyl aspartate specific proteinase-3 (Caspase-3) protein in brain tissue was determined by Western blotting. HE staining was performed to observe pathological changes of brain tissue lesions. Results Compared with the control group, the CPP of the model group, Naoxintong capsule high, medium and low dose group and positive control group were significantly increased (P<0.05), c-fos gene, expressions of Caspase-3 and Bax protein in brain tissue were significantly increased (P<0.05), Bcl-2 expression was significantly decreased (P<0.05). Compared with the model group, CPP of Naoxintong capsule high and medium dose group and positive control group was significantly decreased (P<0.05), c-fos gene, expression of Caspase-3 and Bax protein were significantly decreased (P<0.05), and Bcl-2 expression was significantly increased (P<0.05). HE staining showed that degree of brain cell lesions was reduced. Conclusion Naoxintong Capsule may protect brain cells injury of rats with ketamine addiction by regulating apoptosis of brain tissues.

Key words: Naoxintong capsule, Ketamine, Rat, Brain cell injury

中图分类号:

DF795.1Q95-33

柳小波, 杨梅, 宋佳. 脑心通胶囊对氯胺酮成瘾大鼠脑细胞损伤的保护作用[J]. 实验动物与比较医学, 2019, 39(6): 437-442.

LIU Xiao-bo, YANG Mei, Song Jia. Protective Effect of Naoxintong Capsule on Brain Cell Injury in Rats with Ketamine Addiction[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 437-442.

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脑心通胶囊对氯胺酮成瘾大鼠脑细胞损伤的保护作用

Protective Effect of Naoxintong Capsule on Brain Cell Injury in Rats with Ketamine Addiction

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