鼠诺如病毒特性、免疫学及检测方法研究进展

doi:10.3969/j.issn.1674-5817.2015.05.016

摘要/Abstract

摘要： 鼠诺如病毒(murine norovirus, MNV)是唯一可在体外有效扩增的诺如病毒, 其病毒株的分离及在鼠巨噬细胞RAW264.7细胞的增殖成功为诺如病毒分子机制的研究奠定了基础, 也为人类诺如病毒(HuNoVs)致病机制的研究和疫苗的制备提供了帮助。MNV是小鼠中传播最广泛的感染因子之一, 具嗜免疫细胞性, 对免疫缺陷小鼠可产生致死性, 加强该病毒感染的控制具有重要意义。

关键词: 鼠诺如病毒(MNV), 特性, 机制, 检测

Abstract: Murine norovirus (MNV) is the only norovirus that can be cultivated in vitro. The isolation of the strains and its successful proliferation in the mouse macrophage RAW264.7 cell lay the foundation for molecular mechanism research of norovirus, which also provide help for the pathogenic mechanism research and preparation of vaccine of human norovirus (HuNoVs). MNV is one of the most widely spread infection factors in mice. The replication of murine norovirus in cell culture reveals a tropism for dendritic cells and macrophages. The virus can make lethal effect on immune-deficient mice. It's of great significance to strengthen the control of the virus infection.

Key words: Murine norovirus, Characteristics, Mechanism, Detection

中图分类号:

Q95-33

高洁, 贺争鸣. 鼠诺如病毒特性、免疫学及检测方法研究进展[J]. 实验动物与比较医学, 2015, 35(5): 414-420.

GAO Jie, HE Zheng-ming. Research Progress of Murine Norovirus in Characteristics, Immunology and Detection Methods[J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 414-420.

参考文献

[1] Taube S, Kolawole AO, Hhne M, et al.A mouse model for human norovirus[J]. MBio, 2013, 4(4):1-8.
[2] Baldridge MT, Nice TJ, McCune BT, et al. Commensal microbes and interferon-λ determine persistence of enteric murine norovirus infection[J]. Science, 2015, 347(6219):266-269.
[3] Wobus CE, Karst SM, Thackray LB, et al.Replication of norovirus in cell culture reveals a tropism for dendritic cells and macrophages[J]. PLoS Biol, 2004, 2(12):e432.
[4] Papafragkou E, Hewitt J, Park GW, et al.Challenges of culturing human norovirus in three -dimensional organoid intestinal cell culture models[J]. PLoS One, 2013, 8(6): e63485.
[5] Herod MR, Salim O, Skilton RJ, et al.Expression of the murine norovirus (MNV) ORF1 polyprotein is sufficient to induce apoptosis in a virus-free cell model[J]. PLoS One, 2014,9(3):e90679.
[6] M hler M, K hl W. Aserological survey to evaluate contemporary prevalence of viral agents and mycoplasma pulmonis in laboratorymice and rats in western Europe[J]. Lab Anim(NY), 2009, 38(5):161-165.
[7] Ohsugi T, Matsuura K, Kawabe S, et al.Natural infection of murine norovirus in conventional and specific pathogen-free laboratory mice[J]. Front Microbiol, 2013, 4:12.doi: 10.3389 /fmicb.2013.00012. eCollection 2013.
[8] Karst SM, Wobus CE, Lay M, et al.STAT1-dependent innate immunity to a Norwalk-like virus[J]. Science, 2003, 299(5612), 1575-1578.
[9] Taube S, Rubin JR, Katpally U, et al.High resolution X-ray structure and functional analysis of the murine norovirus 1 capsid protein protruding domain[J]. J Virol , 2010, 84(11): 5695-5705.
[10] Lochridge VP, Jutila KL, Graff JW, et al.Hardy. Epitopes in the P2 domain of norovirus VP1 recognized by monoclonal antibodies that block cell interactions[J]. J Gen Virol, 2005,86(10):2799-2806.
[11] McFadden N, Bailey D, Carrara G, et al. Norovirus regulation of the innate immune response and apoptosis occurs via the product of the alternative open reading frame 4[J]. PLoS Pathog, 2011, 7:e1002413. doi:10.1371/journal.ppat.1002413.
[12] Zhu S, Regev D, Watanabe M, et al. Identification of immune and viral correlates of norovirus protective immunity through comparative study of intra-cluster norovirus strains[J]. PloS Pathog, 2013, 9(9):Le1003592.
[13] Wobus CE, Thackray LB, Virgin HW 4th .Murine norovirus:a model system to study norovirus biology and pathogenesis[J]. J Virol, 2006, 80(11):5104-5112.
[14] Fischer AE, Wu SK, Proescher JB, et al.A high-throughput drop microfluidic system for virus culture and analysis[J]. J Virol Methods, 2015, 213:111-117.
[15] Bertolotti-Ciarlet A, White LJ, Chen R, et al.Structural requirements for the assembly of Norwalk virus-like particles[J]. J Virol, 2002, 76(8):4044-4055.
[16] 袁文, 张钰, 刘忠华, 等. 广东省实验小鼠自然感染鼠诺如病毒的调查[J]. 中国比较医学杂志, 2010, 20(2):78-82.
[17] 李晓波, 付瑞, 岳秉飞, 等. 鼠诺如病毒的体外分离与鉴定[J]. 中国病毒学杂志, 2011, 1(4):293-296.
[18] Thackray LB, Wobus CE, Chachu KA, et al.Murine noroviruses comprising a single genogroup exhibit biological diversity despite limited sequence divergence[J]. J Virol, 2007, 81(19):10460-10473.
[19] Gonzalez-Hernandez MB, Liu T, Blanco LP, et al.Murine norovirus transcytosis across an in vitro polarized murine intestinal epithelial monolayer is mediated by M-Like cells[J]. J Virol, 2012, 87(23):12685-12693.
[20] Henderson KS.Murine norovirus, a recently discovered and highly prevalent viral agent of mice[J]. Lab Anim (NY), 2008, 37(7):314-320.
[21] Xiang Z, Tian S, Tong W, et al.MNV primarily surveillance by a recombin ation VP1-derived ELISA in Beijing area in China[J]. J Immunol Methods, 2014, doi.org/10.1016/j.jim.2014.05.007.
[22] Smith DB, McFadden N, Blundell RJ, et al. Diversity of murine norovirus in wild-rodent populations: species-specific associations suggest an ancient divergence[J]. J Gen Virol, 2012, 93(Pt2):259-66.
[23] Hsu CC, Riley LK, Wills HM, et al.Persistent infection with and serologic cross-reactivity of three novel murine novoviruses[J]. Comp Med, 2006, 56(4):247-251.
[24] Goto K, Hayashimoto N, Yasuda M, et al.Molecular detection of murine norovirus from experimentally and spontaneously infected mice[J]. Exp Anim, 2009, 58(2):135-140.
[25] Compton SR, Paturzo FX, Macy JD.Effect of murine norovirus infection on mouse parvovirus infection[J]. J Am Assoc Lab Anim Sci, 2010, 49(1):11-21.
[26] Chen X, Leach D, Hunter DA, et al.Characterization of intestinal dendritic cells in murine norovirus infection[J].Open Immunol J, 2011, 4:22-30.
[27] Msrionneau S, Ruvoen N, Le Moullac-Vaidye,et al.Norwalk virus binds to histo-blood group antigens present on gastroduodenal epithelial cells of secretor individuals[J]. Gastroenterolo-gy, 2002, 122(7):1967-1977.
[28] Caddy S, Breiman A, Ie Pendu J, et al.Genogroup IV and VI canine noroviruses interact with histo-blood group antigens[J]. J Virol, 2014, 88(18):10377-10391.
[29] Taube S, Perry JW, McGreevy E, et al. Murine norovirus bind glycolipid and glycoprotein attachment receptors in a strain-dependent manner[J]. J Virol, 2012, 86(10):5584-5593.
[30] Taube S, Perry JW, Yetming K, et al.Ganglioside-linked terminal sialic acid moieties on murine macrophages function as attachment receptors for murine noroviruses[J]. J Virol, 2009, 83(9):4092-4101.
[31] Strong DW, Thackray LB, Smith TK, et al.Protruding domain of capsid protein is necessary and sufficient to determine murine norovirus replication and pathogenesis in vivo[J]. J Virol, 2012, 86(6):2950-2958.
[32] Yunus MA, Lin X, Bailey D, et al.The murine norovirus (MNV)core subgenomic RNA promoter consists of a stable stem-loop that can direct accurate initiation of RNA synthesis[J]. J Virol, 2014, doi:10.1128/JVI.02432-14.
[33] Nice TJ, Baldridge MT, McCune BT, et al. Interferon-λ cures persistent murine norovirus infection in the absence of adaptive immunity[J]. Science, 2015, 347(6219):269-273.
[34] Fang H, Tan M, Xia M, et al.Norovirus P particle efficiently elicits innate, humoral and cellular immunity[J]. PLoS One,2013, 8(4):e63269.
[35] Bailey D, Thackray LB, Goodfellow IG.A single amino acid substitution in the murine norovirus capsid protein is sufficient for attenuation in vivo[J]. J Virol, 2008, 82(15):7725-7728.
[36] Tomov VT, Osborne LC, Dolfi DV, et al.Persistent enteric murine norovirus infection is associated with functionally suboptimal virus-specific CD8 T cell response[J]. J Virol,2013, 87(12):7015-7031.
[37] Furman LM, Maaty WS, Petersen LK, et al . Cysteine protease activation and apoptosis in murine norovirus infection[J]. Virol J, 2009, 6:139-149.
[38] Kolawole AO, Li M, Xia C, et al.Flexibility in surface-exposed loops in a virus capsid mediates escape from antibody neutralization[J]. J Virol, 2014, 88(8):4543-4557.
[39] 吴冰珊, 严延生. 诺如病毒衣壳蛋白表达的研究进展[J]. 中国人兽共患病学报, 2009, 25(7):673-675.
[40] Hus CC, Wobus CE, Steffen EK, et al.Development of a microsphere-based serologic mutiplexd fluorescent immunoassay and a reverse transcriptase PCR assay to detect murine norovirus 1 infection in mice[J]. Clin Diagn Lab Immunol , 2005, 12(10):1145-1151.
[41] Kitajima M, Oka T, Tohya Y, et al.Development of a broadly reactive nested transcription-PCR assay to detect murine noroviruses, and investigation of the prevalen ce of murine noroviruses in laboratory mice in Japan[J]. Microbiol Immunol, 2009, 53(9):531-534.
[42] Tajima M, Kotani Y, Kurosawa T, et al.Pitfalls in mouse norovirus (MNV)detection in fecal samples using RT-PCR,and construction of new MNV-specific primers[J]. Exp Anim, 2013, 62(2):127-135.
[43] 田胜男, 刘云波. 鼠诺如病毒概述[J]. 中国比较医学杂志, 2013, 23(7):68-71.
[44] Kitajima M, Oka T, Takagi H, et al.Development and application of a broadly reactive real-time reverse transcription-PCR assay for detection of murine norovirus[J]. J Virol Methods, 2010, 169(2):269-273.
[45] Hanaki K, Ike F, Kajita A, et al.A Broadly reactive one-step SYBR green I real-time RT-PCR assay for rapid detection of murine norovirus[J]. PLoS One, 2014, 9(5):e98108.
[46] Oliver SL, Dastjerdi AM, Wong S, et al.Molecular characterization of bovine enteric calicibiruses :a distinct third genogroup of noroviruses (Norwalk-like viruses) unlikely to be of risk to humans[J]. J Virol, 2003, 77(9):2789-2798.
[47] Widdowson MA, Rockx B, Schepp R, et al.Detection of serum antibodies to bovine norovirus in veterinarians and the general population in the Netherlands[J]. J Med Virol,2005. 76(1):119-128.
[48] Wang QH, Han MG, Cheetham S, et al.Porcine noroviruses related to human noroviruses[J]. Emerg Infect Dis, 2005, 11(12):1874-1881.
[49] Kageyama T, Shinohara M, Uchida K, et al.Coexistence of multiple genotypes, including newly identified genotypes, in outbreaks of gastroenteritis due to norovirus in Japan[J]. J Clin Microbiol, 2004, 42(7):2988-2995.
[50] 向丹丹. RT-PCR检测实验小鼠自然和实验感染鼠诺瓦克病毒及病毒株分离[D]. 上海: 东华大学, 2013.
[51] Kernbauer E, Dinq Y, Cadwell K.An enteric virus can replace the beneficial function of commensal bacteria[J]. Nature,2014, 516(7529):94-98.
[52] 北京市疾控中心. 北京市疾控中心2015年第12周疫情周报[DB/OL]. 2015, 03-26. http://www.bjcdc.org/article/39899/2015/3/1427340532805.html