实验动物与比较医学 ›› 2022, Vol. 42 ›› Issue (2): 102-110.DOI: 10.12300/j.issn.1674-5817.2021.102

所属专题: 实验动物设施设备

• 实验动物与生物安全专题 • 上一篇    下一篇

自主研发生物安全型换笼工作台的性能检测与评价

苏世博(), 马文杰, 王伟, 郭慧娟, 张贺, 王梓, 康雷, 刘守琦, 刘怀然, 司昌德, 苟春天, 张伟, 陈洪岩()   

  1. 中国农业科学院哈尔滨兽医研究所, 兽医生物技术国家重点实验室, 国家禽类实验动物资源库, 黑龙江省实验动物与比较医学重点实验室, 哈尔滨 150069
  • 收稿日期:2021-06-02 修回日期:2022-02-28 出版日期:2022-04-25 发布日期:2022-05-07
  • 通讯作者: 陈洪岩(1963—),男,研究员,研究方向:实验动物与比较医学。E-mail:chenhongyan@caas.cn
  • 作者简介:苏世博(1996—),男,硕士研究生,研究方向:实验动物与比较医学。E-mail:270141601@qq.com
  • 基金资助:
    国家重点研发计划项目: BSL-4实验室IVC笼盒更换工作台的研制及应用(2018YFC1200301);手套箱式生物隔离器和感染动物隔离设备的研发(2016YFC1201401)

Performance Test and Evaluation of Self-developed Biosafety Cage Changing Workbench

Shibo SU(), Wenjie MA, Wei WANG, Huijuan GUO, He ZHANG, Zi WANG, Lei KANG, Shouqi LIU, Huairan LIU, Changde SI, Chuntian GOU, Wei ZHANG, Hongyan CHEN()   

  1. State Key Laboratory of Veterinary Biotechnology, National Poultry Laboratory Animal Resource Center, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Harbin 150069, China
  • Received:2021-06-02 Revised:2022-02-28 Online:2022-04-25 Published:2022-05-07
  • Contact: CHEN Hongyan, E-mail: chenhongyan@caas.cn

摘要:

目的 对自主研发的生物安全型换笼工作台进行功能检测与安全性评价。方法 依据YY 0569—2011《Ⅱ级生物安全柜》、RB/T 199—2015《实验室设备生物安全性能评价技术规范》等标准对自主研发的由深圳泓腾生物科技有限公司生产的生物安全型换笼工作台(HT-1303)的各项性能指标进行检测,并判定其有效性及安全性。结果 换笼工作台的主体外观完整性和稳定性、工作区洁净度、流入气流流速、下降气流流速、照度、噪声、气流流向、高效过滤器等检测效果良好,达到Ⅱ级生物安全柜标准。污染物品回收舱及器物表面灭菌舱的气密性效果良好,其尺寸、灭菌笼盒数量等还需进一步改进。结论 本团队研制的生物安全型换笼工作台可实现整机正常运行,其各项指标合格,能够完成负压环境下独立通风笼具的更换,并保证操作者、实验对象及环境的安全。

关键词: 换笼工作台, 性能检测, 生物安全

Abstract:

Objective To test the function and evaluate the safety of the self-developed biosafety cage changing workbench.Methods According to the criteria of YY 0569-2011 "Class II Biological Safety Cabinet" and RB/T 199-2015 "Laboratory Equipment Biosafety Performance Evaluation Technical Specifications", we detected the various performance indicators of the cage changing workbench (HT-1303), which was independently developed and produced by Shenzhen Hongteng Biological Technology Co., LTD, and evaluated the safety and validity.Results The appearance integrity and stability of the main body of the biosafety cage changing workbench, the cleanliness of the working area, the velocity of the inflow airflow, the velocity of the descending airflow, the illuminance, the noise level, the airflow direction, the high-efficiency particulate air filter were at good condition, reaching the standards of level II biological safety cabinet. The airtightness of the contaminated goods recovery cabin and the sterilization chamber of utensil surface was good, but the size and quantity of the sterilized cage box need to be further improved.Conclusion The biosafety cage changing workbench developed by our team can be operated properly in whole. It can complete the replacement of individually ventilated cages in this workbench under negative pressure environment. Our workbench also can ensure the safety of operators, environment and experimental subjects.

Key words: Biosafety cage changing workbench, Performance test, Biosafety

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