水溶性氮酮对斑马鱼胚胎药物渗透及致畸性作用的影响

doi:10.3969/j.issn.1674-5817.2011.02.008

实验动物与比较医学 ›› 2011, Vol. 31 ›› Issue (2): 108-110.DOI: 10.3969/j.issn.1674-5817.2011.02.008

水溶性氮酮对斑马鱼胚胎药物渗透及致畸性作用的影响

陈锡强¹, 韩利文^1,2, 刘可春¹, 侯海荣¹, 王希敏¹, 何秋霞¹

1.山东省科学院生物研究所, 济南 250014;
2.天津中医药大学, 天津 300193

收稿日期:2010-07-13 出版日期:2011-04-25 发布日期:2011-04-25
作者简介:陈锡强(1972-), 男, 博士, 研究方向: 天然产物抗肿瘤筛选。E-mail:cxqq_1972@163.com
基金资助:
山东省科学院博士基金资助项目(No SY2007BS10)

Effects of Azone on Osmotic Absorption of Resveratrol in Zebrafish

CHEN Xi-qiang¹, HAN Li-wen^1,2, LIU Ke-chun¹, HOU Hai-rong¹, WANG Xi-min¹, HE Qiu-xia¹

1. Biology Institute of Shandong Academy of Sciences, Jinan;
2.Tianjin Chinese medicine University, Tianjin 250014, China;300193

Received:2010-07-13 Online:2011-04-25 Published:2011-04-25

摘要/Abstract

摘要： 目的研究水溶性氮酮对白藜芦醇透过斑马鱼胚胎性能与斑马鱼致畸性的影响。方法利用对斑马鱼胚胎发育致畸作用,考察氮酮在斑马鱼筛选模型上的适用性,确定氮酮的使用剂量; 利用氮酮的透皮促进作用, 观察对白藜芦醇抑制斑马鱼节间血流的影响。结果在0.01~0.5 μg/ml范围内氮酮对斑马鱼较安全,0.5 μg/ml溶性氮酮可显著促进斑马鱼对白藜芦醇的透过,斑马鱼节间血管的抑制率达到了22.9%。结论浓度高于0.5 μg/ml的水溶性氮酮对斑马鱼胚胎的发育有明显致畸作用; 0.5 μg/ml的氮酮对白藜芦醇的斑马鱼胚胎透过效果最佳。

关键词: 氮酮, 白藜芦醇, 透皮吸收, 斑马鱼

Abstract: Objective To study the effects of water-solubility azone on the transdermal absorption of resveratrol and teratogenicity. Methods The formation of neovascularization was inhibited by using resveratrol, the number of zebrafish dorsal aorta intersegmental blood flow were calculated added with water- solubility azone under fluorescent microscope, and teratogenic-action effects were recorded by water-solubility azone added for 24 h. Results The water -solubility azone at the coneentration of 0.01~0.5 µg/ml were safe to zebrafish embry, and the 0.5 µg/ml water-solubility azone showed significant transdermal absorption of resveratrol in zebrafish, intersegmental vessel number of dorsal aorta inhibition ratio could be 22.9%, the incidence of deformity can be occured. Conclusions The water-soluble azone with the Concentrations over 0.5 µg/ml have significant teratogenic effects on zebrafish embryos; and the 0.5 µg/ml water-solubility azone shows the optimal transdermal absorption of the resveratrol.

Key words: Azone, Resveratrol, Percutaneous absorption, Zebrafish

中图分类号:

Q95-33

陈锡强, 韩利文, 刘可春, 侯海荣, 王希敏, 何秋霞. 水溶性氮酮对斑马鱼胚胎药物渗透及致畸性作用的影响[J]. 实验动物与比较医学, 2011, 31(2): 108-110.

CHEN Xi-qiang, HAN Li-wen, LIU Ke-chun, HOU Hai-rong, WANG Xi-min, HE Qiu-xia. Effects of Azone on Osmotic Absorption of Resveratrol in Zebrafish[J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 108-110.

参考文献

[1] Thaloor D, Singh AK, Sidhu GS, et al.Inhibition of angiogenic differentiation of human umbilical vein endothelial cells by curcumin[J]. Cell Growth Differ, 1998, 9(4):305-312.
[2] Westerfield M, Wegner J, Jegalian BG.Specific activation of mammalian Hox promoters in mosaic transgenic zebrafish[J]. Genes Dev, 1992, 6(4):591-598.
[3] Rubinstein AL.Zebrafish:from disease modeling to drug discovery[J].Current Opinion in Drug Discovery & Development, 2003, 6(2):218-223.
[4] Child S, chen JN, Garrity DM,et al.Patterning of angiogenesis in the zebrafish embryo[J]. Development, 2002, 12(9):973-982.
[5] Cross LM, Cook MA, Lin S, et al.Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay[J].Arterioscler Thromb Vasc Biol, 2003, 23:911-912.
[6] Mirella B, Domenico R, Stefania N, et al.Antiangiogenic and Vascular-Targeting Activity of the Microtubule-Destabilizing trans-Resveratrol Derivative 3,5,4-Trimethoxystilbene[J]. Mol Pharmacol, 2005, 67:1451-1459.
[7] 王思锋, 王希敏, 侯海荣, 等. 有机助溶剂对血管生成斑马鱼模型的适用性研究[J]. 实验动物与比较医学, 2008, 28(4):238-241.
[8] Igura K, Ohta T, Kuroda Y, et al. Resveratrol and quercetin inhibit angiogenesis in vitro. Cancer Lett, 2001, 28; 171(1):11-16.
[9] LIN ming-tsan,men-luh YEN, ia-yi LIN, et al. Inhibition of vascular endothelial growth factor-induced angiogenesis by resveratrol through interruption of Src-dependent vascular endothelial cadherin tyrosine phosphorylation[J]. Mol Pharmacol, 2003, 5(64) :1029-1036.
[10] 庆林. 透皮吸收促进剂的研究应用[J].中华实用中西医杂志, 2001, 1(14):11.
[11] ItoY, OgisoT, IwakiM. Thermodynamic study on enhancementof percutaneous penetration of drugs by azone[J]. Pharmacobiodyn,1988, 11(11): 749-757.
[12] Afouna M.I,Fincher T K, Zaghlou A.et al. Effect of Azone upon the in vivo antiviralefficacy ofcidofoviroracyclo vir topical formulations in treatment/prevention of cutaneous HSV-1 infections and its correlation with skin target site free drug concentration in hairlessmice[J]. Pharm,2003, 253(I-2):159-168.

[1]	林金杏, 王新栋, 白雪兵, 冯丽萍, 谢淑武, 陈秋生. 成年斑马鱼体肾的微细结构及其外泌体的分布鉴定[J]. 实验动物与比较医学, 2023, 43(5): 531-540.
[2]	杨晋娴, 王淑娟, 翟金云, 朱顺星. Micall2a基因表达下调抑制斑马鱼血管发育[J]. 实验动物与比较医学, 2023, 43(3): 282-287.
[3]	谭志刚, 刘锦信, 郑楚雅, 廖文峰, 冯露平, 彭红丽, 严秀, 卓振建. 神经母细胞瘤动物模型研究进展与应用[J]. 实验动物与比较医学, 2023, 43(3): 288-296.
[4]	金仕容, 华叶, 訾化星, 杜旭飞, 卜纪雯. 一种显著提高实验用斑马鱼繁殖效率和使用寿命的优化养殖方案[J]. 实验动物与比较医学, 2023, 43(3): 297-306.
[5]	张晓节, 蒋磊. 基于网络药理学探究白藜芦醇抗大鼠心肌缺血再灌注损伤的分子机制[J]. 实验动物与比较医学, 2020, 40(6): 496-505.
[6]	江霞, 钱豪杰, 魏迅, 郑羽旋, 周正宇. 斑马鱼糖尿病模型的构建及应用进展[J]. 实验动物与比较医学, 2020, 40(6): 547-552.
[7]	邸亚男, 朱丽英, 钱雯, 潘卫. 斑马鱼作为模式动物在人类眼睛疾病研究中的应用[J]. 实验动物与比较医学, 2020, 40(5): 440-.
[8]	王雪, 刘可春, 杨学亮, 马玉奎, 张云. 冈田酸对斑马鱼幼鱼神经行为功能的影响[J]. 实验动物与比较医学, 2020, 40(3): 190-.
[9]	李英娘, 戴玮, 盛健. 斑马鱼甲状腺肿大病例分析[J]. 实验动物与比较医学, 2019, 39(6): 462-466.
[10]	王俐梅, 张建, 刘忠华, 杨威. 视网膜母细胞瘤斑马鱼模型及rb1基因研究进展[J]. 实验动物与比较医学, 2019, 39(3): 244-248.
[11]	周斌, 盛哲津, 冯琛卓, 李利妹. 一种体内实时观察黑色素瘤的斑马鱼模型[J]. 实验动物与比较医学, 2018, 38(1): 22-28.
[12]	田芳, 王玉柱, 夏敏杰, 孙冰, 丁训城, 李卫华, 许慧慧, 胡晶莹. 实验用斑马鱼成鱼内脏组织病理学检查方法的优化[J]. 实验动物与比较医学, 2017, 37(6): 465-469.
[13]	金璐, 李燕, 李志操, 吴西军, 周艳华, 何志旭, 舒莉萍. Irx5a基因过表达对斑马鱼胚胎早期造血的影响[J]. 实验动物与比较医学, 2017, 37(4): 309-314.
[14]	林金杏, 冯丽萍, 胡建华, 高诚. 斑马鱼鳍和鳞片色素细胞的显微观察[J]. 实验动物与比较医学, 2017, 37(2): 94-101.
[15]	张城达, 张丽丽, 张立将, 陈云祥. 阿霉素致斑马鱼心血管毒性模型的建立[J]. 实验动物与比较医学, 2015, 35(5): 362-366.

水溶性氮酮对斑马鱼胚胎药物渗透及致畸性作用的影响

Effects of Azone on Osmotic Absorption of Resveratrol in Zebrafish

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价