视网膜母细胞瘤斑马鱼模型及rb1基因研究进展

doi:10.3969/j.issn.1674-5817.2019.03.013

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (3): 244-248.DOI: 10.3969/j.issn.1674-5817.2019.03.013

视网膜母细胞瘤斑马鱼模型及rb1基因研究进展

王俐梅^1,2,4, 张建³, 刘忠华², 杨威^1,4

1.广东省生物资源应用研究所, 广东省动物保护与资源利用重点实验室,广东省野生动物保护与利用公共实验室, 广州510260;
2.华南农业大学兽医学院, 广州510642;
3.广州医科大学基础医学院, 广州 511436;
4.广东莱恩医药研究院有限公司, 广东省药物非临床评价研究企业重点实验室, 广州 510990

收稿日期:2018-11-16 出版日期:2019-06-25 发布日期:2021-01-29
作者简介:王俐梅(1986-),女,硕士研究生,执业兽医师,主要从事建立动物模型研究。E-mail:door20@126.com
基金资助:
广东省科学院创新药物安全性评价研究团队(2016GDASRC-0104),国家自然科学基金青年项目(11704082),广东省药物非临床评价研究企业重点实验室(2018B030323024),广东省生物资源应用研究所研究生和博士后研究人员培养基金(GIABR-pyjj201605)

Research Progress on Zebrafish Model of Retinoblastoma and rb1 Gene

WANG Li-mei^1,2,4, ZHANG Jian³, LIU Zhong-hua², YANG Wei^1,4

1. Guangdong Institute of Applied Biological Resources, Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangzhou 510260, China;
2. College of Veterinary Medicine of South China Agriculture University, Guangzhou 510642, China;
3. School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, China;
4. Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-clinical Evaluation and Research, Guangzhou 510990, China

Received:2018-11-16 Online:2019-06-25 Published:2021-01-29

摘要/Abstract

摘要： 视网膜母细胞瘤(Retinoblastoma,RB)是一种常见的发生于婴幼儿眼内的原发恶性肿瘤,大量的研究表明,rb1基因的改变是导致RB发生的主要原因。新型模式生物斑马鱼最近几年被开发成为研究RB的动物模型,本文在介绍rb1基因与RB疾病相关研究新进展的同时,综述了已有报道的RB斑马鱼模型建立方法,包括异种移植法、基因突变法和TALEN基因编辑法,并进一步将RB斑马鱼模型与已有报道的小鼠、大鼠、兔、非洲爪蟾RB动物模型进行了对比。

关键词: 视网膜母细胞瘤(RB), 斑马鱼, rb1基因

Abstract: Retinoblastoma (RB) is a common primary malignant tumor that occurs in the eyes of infants and young children. A large number of studies have shown that the change of rb1 gene is the main cause of RB occurrence. The zebrafish RB model is a novel animal model for studying RB. In this paper, the latest research results of rb1 gene, as well as the zebrafish RB model produced by xenograft method, gene mutation method, and TALEN gene editing method are reviewed. Furthermore, the RB zebrafish model was compared with the four kinds of reported RB animal models that were raised on mice, rat, rabbit or xenopus.

Key words: Retinoblastoma (RB), Zebrafish, rb1 gene

中图分类号:

Q95-33

王俐梅, 张建, 刘忠华, 杨威. 视网膜母细胞瘤斑马鱼模型及rb1基因研究进展[J]. 实验动物与比较医学, 2019, 39(3): 244-248.

WANG Li-mei, ZHANG Jian, LIU Zhong-hua, YANG Wei. Research Progress on Zebrafish Model of Retinoblastoma and rb1 Gene[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 244-248.

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视网膜母细胞瘤斑马鱼模型及rb1基因研究进展

Research Progress on Zebrafish Model of Retinoblastoma and rb1 Gene

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