›› 1999, Vol. 19 ›› Issue (2): 117-118.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

无毛KM小鼠近交培育的阶段性成果

  

  1. 北京医科大学实验动物科学部,北京 100083
  • 收稿日期:1998-10-19 出版日期:1999-01-25 发布日期:2013-03-19

Progress of Inbreeding of Hairless KM Mice

  • Received:1998-10-19 Online:1999-01-25 Published:2013-03-19

摘要: 将本部发现的一只无毛KM小鼠进行回交和自交后,自1995年2月起采取全同胞兄妹交配的方法,培育至今已达F18。代间隔平均为71.5d(55~116d)。以近交F18与DBA/2交配进行毛色基因测试,其杂交1代毛色一致,提示F18无毛小鼠已达到较高的基因纯合度。该无毛小鼠自4周龄起全身开始脱毛,至5周龄全身基本无毛,6周龄后又长出稀短的毛。该鼠已经剖宫取胎净化为清洁级。

关键词: 无毛小鼠, 近交, 培育, 清洁级

Abstract: The hairless mutant mouse was found in KM closed colony. The “hairless” gene was retained by backcrossing and brother X sister mating system. The inbreeding had progressed to the 18th generation since February 1995. The average generation interval was 71.5 days (55-116 days). The result of coat color test crossing of the F18 with DBA/2 showed that the color genes in F18 were homogenous. The hairless mice start to shed at the age of 4 weeks and lose almost all hairs at the age of 5 weeks. The sparse and short hairs gradually appear after the age of 6 weeks. Clean hairle'ss mice have been derived through caesarean section.

Key words: "Hairless mouse, Inbreeding, KM mouse "